Experiment: R2A with nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01445 and CA265_RS01450 are separated by 177 nucleotides CA265_RS01450 and CA265_RS01455 are separated by 4 nucleotides
CA265_RS01445: CA265_RS01445 - hypothetical protein, at 367,303 to 368,619
_RS01445
CA265_RS01450: CA265_RS01450 - hypothetical protein, at 368,797 to 371,172
_RS01450
CA265_RS01455: CA265_RS01455 - MFS transporter, at 371,177 to 372,418
_RS01455
Position (kb) 368
369
370
371
372 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 367.854 kb on + strand, within CA265_RS01445 at 367.865 kb on - strand, within CA265_RS01445 at 367.925 kb on - strand, within CA265_RS01445 at 367.925 kb on - strand, within CA265_RS01445 at 367.925 kb on - strand, within CA265_RS01445 at 367.971 kb on - strand, within CA265_RS01445 at 368.038 kb on - strand, within CA265_RS01445 at 368.052 kb on + strand, within CA265_RS01445 at 368.073 kb on + strand, within CA265_RS01445 at 368.073 kb on + strand, within CA265_RS01445 at 368.074 kb on - strand, within CA265_RS01445 at 368.121 kb on + strand, within CA265_RS01445 at 368.206 kb on + strand, within CA265_RS01445 at 368.223 kb on - strand, within CA265_RS01445 at 368.357 kb on - strand, within CA265_RS01445 at 368.358 kb on + strand, within CA265_RS01445 at 368.443 kb on - strand, within CA265_RS01445 at 368.476 kb on + strand, within CA265_RS01445 at 368.556 kb on + strand at 368.566 kb on - strand at 368.566 kb on - strand at 368.602 kb on + strand at 368.624 kb on + strand at 368.649 kb on + strand at 368.657 kb on - strand at 368.661 kb on - strand at 368.681 kb on + strand at 368.681 kb on + strand at 368.715 kb on + strand at 368.725 kb on + strand at 368.758 kb on + strand at 368.767 kb on - strand at 368.795 kb on + strand at 368.843 kb on + strand at 368.853 kb on + strand at 368.896 kb on + strand at 369.019 kb on + strand at 369.019 kb on + strand at 369.020 kb on - strand at 369.058 kb on + strand, within CA265_RS01450 at 369.059 kb on - strand, within CA265_RS01450 at 369.064 kb on - strand, within CA265_RS01450 at 369.084 kb on - strand, within CA265_RS01450 at 369.144 kb on + strand, within CA265_RS01450 at 369.162 kb on + strand, within CA265_RS01450 at 369.163 kb on - strand, within CA265_RS01450 at 369.278 kb on - strand, within CA265_RS01450 at 369.316 kb on - strand, within CA265_RS01450 at 369.316 kb on - strand, within CA265_RS01450 at 369.408 kb on + strand, within CA265_RS01450 at 369.429 kb on + strand, within CA265_RS01450 at 369.551 kb on + strand, within CA265_RS01450 at 369.589 kb on - strand, within CA265_RS01450 at 369.662 kb on - strand, within CA265_RS01450 at 369.694 kb on + strand, within CA265_RS01450 at 369.695 kb on - strand, within CA265_RS01450 at 369.715 kb on + strand at 369.735 kb on - strand, within CA265_RS01450 at 369.777 kb on + strand, within CA265_RS01450 at 369.816 kb on + strand, within CA265_RS01450 at 369.836 kb on + strand at 369.854 kb on - strand, within CA265_RS01450 at 369.857 kb on + strand, within CA265_RS01450 at 369.909 kb on + strand, within CA265_RS01450 at 370.033 kb on - strand, within CA265_RS01450 at 370.204 kb on + strand, within CA265_RS01450 at 370.204 kb on + strand, within CA265_RS01450 at 370.205 kb on - strand, within CA265_RS01450 at 370.205 kb on - strand, within CA265_RS01450 at 370.249 kb on - strand, within CA265_RS01450 at 370.257 kb on + strand, within CA265_RS01450 at 370.333 kb on - strand, within CA265_RS01450 at 370.333 kb on - strand, within CA265_RS01450 at 370.396 kb on - strand, within CA265_RS01450 at 370.483 kb on - strand, within CA265_RS01450 at 370.483 kb on - strand, within CA265_RS01450 at 370.486 kb on - strand, within CA265_RS01450 at 370.651 kb on - strand, within CA265_RS01450 at 370.767 kb on - strand, within CA265_RS01450 at 370.809 kb on + strand, within CA265_RS01450 at 370.810 kb on - strand, within CA265_RS01450 at 370.839 kb on - strand, within CA265_RS01450 at 370.843 kb on + strand, within CA265_RS01450 at 370.843 kb on + strand, within CA265_RS01450 at 370.917 kb on - strand, within CA265_RS01450 at 370.917 kb on - strand, within CA265_RS01450 at 371.027 kb on + strand at 371.045 kb on - strand at 371.058 kb on + strand at 371.058 kb on + strand at 371.126 kb on + strand at 371.126 kb on + strand at 371.153 kb on + strand at 371.165 kb on - strand at 371.165 kb on - strand at 371.165 kb on - strand at 371.170 kb on + strand at 371.210 kb on + strand at 371.211 kb on - strand at 371.211 kb on - strand at 371.219 kb on - strand at 371.219 kb on - strand at 371.260 kb on + strand at 371.260 kb on + strand at 371.260 kb on + strand at 371.261 kb on - strand at 371.265 kb on - strand at 371.265 kb on - strand at 371.265 kb on - strand at 371.341 kb on - strand, within CA265_RS01455 at 371.354 kb on - strand, within CA265_RS01455 at 371.409 kb on + strand, within CA265_RS01455 at 371.439 kb on + strand, within CA265_RS01455 at 371.440 kb on - strand, within CA265_RS01455 at 371.449 kb on + strand, within CA265_RS01455 at 371.453 kb on - strand, within CA265_RS01455 at 371.507 kb on + strand, within CA265_RS01455 at 371.508 kb on - strand, within CA265_RS01455 at 371.508 kb on - strand, within CA265_RS01455 at 371.566 kb on + strand, within CA265_RS01455 at 371.566 kb on + strand, within CA265_RS01455 at 371.606 kb on - strand, within CA265_RS01455 at 371.606 kb on - strand, within CA265_RS01455 at 371.719 kb on - strand, within CA265_RS01455 at 371.770 kb on + strand, within CA265_RS01455 at 371.824 kb on + strand, within CA265_RS01455 at 371.825 kb on - strand, within CA265_RS01455 at 371.825 kb on - strand, within CA265_RS01455 at 371.835 kb on + strand, within CA265_RS01455 at 371.848 kb on + strand, within CA265_RS01455 at 371.854 kb on + strand, within CA265_RS01455 at 371.928 kb on + strand, within CA265_RS01455 at 371.933 kb on - strand, within CA265_RS01455 at 371.985 kb on + strand, within CA265_RS01455 at 371.985 kb on + strand, within CA265_RS01455 at 372.013 kb on - strand, within CA265_RS01455 at 372.021 kb on - strand, within CA265_RS01455 at 372.023 kb on + strand, within CA265_RS01455 at 372.049 kb on - strand, within CA265_RS01455 at 372.052 kb on + strand, within CA265_RS01455 at 372.053 kb on - strand, within CA265_RS01455 at 372.053 kb on - strand, within CA265_RS01455 at 372.085 kb on - strand, within CA265_RS01455 at 372.085 kb on - strand, within CA265_RS01455 at 372.085 kb on - strand, within CA265_RS01455 at 372.125 kb on + strand, within CA265_RS01455 at 372.125 kb on + strand, within CA265_RS01455 at 372.144 kb on + strand, within CA265_RS01455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 6 mM remove 367,854 + CA265_RS01445 0.42 +1.0 367,865 - CA265_RS01445 0.43 -1.3 367,925 - CA265_RS01445 0.47 -2.2 367,925 - CA265_RS01445 0.47 +0.2 367,925 - CA265_RS01445 0.47 -0.3 367,971 - CA265_RS01445 0.51 +0.1 368,038 - CA265_RS01445 0.56 +0.1 368,052 + CA265_RS01445 0.57 -1.2 368,073 + CA265_RS01445 0.58 -0.6 368,073 + CA265_RS01445 0.58 -0.9 368,074 - CA265_RS01445 0.59 +0.3 368,121 + CA265_RS01445 0.62 -1.4 368,206 + CA265_RS01445 0.69 -3.0 368,223 - CA265_RS01445 0.70 -1.0 368,357 - CA265_RS01445 0.80 -0.8 368,358 + CA265_RS01445 0.80 +0.5 368,443 - CA265_RS01445 0.87 +0.3 368,476 + CA265_RS01445 0.89 +0.9 368,556 + -0.7 368,566 - -3.2 368,566 - +0.5 368,602 + +1.3 368,624 + -0.9 368,649 + -1.1 368,657 - +1.5 368,661 - -2.9 368,681 + +0.5 368,681 + +0.3 368,715 + -0.2 368,725 + -1.5 368,758 + -3.2 368,767 - -1.6 368,795 + -2.6 368,843 + +0.4 368,853 + -1.9 368,896 + -1.2 369,019 + -2.0 369,019 + +0.8 369,020 - +0.1 369,058 + CA265_RS01450 0.11 +0.5 369,059 - CA265_RS01450 0.11 -0.1 369,064 - CA265_RS01450 0.11 -0.3 369,084 - CA265_RS01450 0.12 -3.1 369,144 + CA265_RS01450 0.15 -1.8 369,162 + CA265_RS01450 0.15 -1.5 369,163 - CA265_RS01450 0.15 -1.0 369,278 - CA265_RS01450 0.20 -0.1 369,316 - CA265_RS01450 0.22 +0.2 369,316 - CA265_RS01450 0.22 +2.4 369,408 + CA265_RS01450 0.26 -1.9 369,429 + CA265_RS01450 0.27 +0.1 369,551 + CA265_RS01450 0.32 +0.9 369,589 - CA265_RS01450 0.33 +0.6 369,662 - CA265_RS01450 0.36 -0.1 369,694 + CA265_RS01450 0.38 +0.7 369,695 - CA265_RS01450 0.38 +1.5 369,715 + +0.2 369,735 - CA265_RS01450 0.39 +1.1 369,777 + CA265_RS01450 0.41 -0.0 369,816 + CA265_RS01450 0.43 -1.3 369,836 + +1.2 369,854 - CA265_RS01450 0.44 +2.0 369,857 + CA265_RS01450 0.45 +0.3 369,909 + CA265_RS01450 0.47 -0.5 370,033 - CA265_RS01450 0.52 +1.4 370,204 + CA265_RS01450 0.59 -0.3 370,204 + CA265_RS01450 0.59 +1.1 370,205 - CA265_RS01450 0.59 -1.7 370,205 - CA265_RS01450 0.59 -3.4 370,249 - CA265_RS01450 0.61 -1.6 370,257 + CA265_RS01450 0.61 -0.1 370,333 - CA265_RS01450 0.65 -0.3 370,333 - CA265_RS01450 0.65 +0.9 370,396 - CA265_RS01450 0.67 -0.7 370,483 - CA265_RS01450 0.71 +0.9 370,483 - CA265_RS01450 0.71 +3.0 370,486 - CA265_RS01450 0.71 +1.8 370,651 - CA265_RS01450 0.78 +0.1 370,767 - CA265_RS01450 0.83 +1.0 370,809 + CA265_RS01450 0.85 +0.1 370,810 - CA265_RS01450 0.85 -2.3 370,839 - CA265_RS01450 0.86 +1.1 370,843 + CA265_RS01450 0.86 -0.6 370,843 + CA265_RS01450 0.86 -1.6 370,917 - CA265_RS01450 0.89 +0.4 370,917 - CA265_RS01450 0.89 +1.3 371,027 + +0.3 371,045 - -2.4 371,058 + +0.5 371,058 + +0.1 371,126 + +1.0 371,126 + +0.8 371,153 + -3.9 371,165 - +0.6 371,165 - -0.2 371,165 - +0.6 371,170 + -0.2 371,210 + -1.5 371,211 - +0.1 371,211 - -0.2 371,219 - -2.7 371,219 - +0.8 371,260 + -2.0 371,260 + +1.9 371,260 + -0.9 371,261 - +0.9 371,265 - +1.0 371,265 - -3.3 371,265 - -0.9 371,341 - CA265_RS01455 0.13 -0.4 371,354 - CA265_RS01455 0.14 +3.5 371,409 + CA265_RS01455 0.19 +1.4 371,439 + CA265_RS01455 0.21 -0.2 371,440 - CA265_RS01455 0.21 -1.3 371,449 + CA265_RS01455 0.22 -1.8 371,453 - CA265_RS01455 0.22 -0.5 371,507 + CA265_RS01455 0.27 +0.5 371,508 - CA265_RS01455 0.27 -0.2 371,508 - CA265_RS01455 0.27 -0.5 371,566 + CA265_RS01455 0.31 -1.8 371,566 + CA265_RS01455 0.31 +0.1 371,606 - CA265_RS01455 0.35 -1.9 371,606 - CA265_RS01455 0.35 +0.6 371,719 - CA265_RS01455 0.44 -1.3 371,770 + CA265_RS01455 0.48 -1.0 371,824 + CA265_RS01455 0.52 -2.0 371,825 - CA265_RS01455 0.52 +1.0 371,825 - CA265_RS01455 0.52 -0.2 371,835 + CA265_RS01455 0.53 -2.0 371,848 + CA265_RS01455 0.54 +1.4 371,854 + CA265_RS01455 0.55 -1.3 371,928 + CA265_RS01455 0.60 -1.1 371,933 - CA265_RS01455 0.61 +0.7 371,985 + CA265_RS01455 0.65 -0.4 371,985 + CA265_RS01455 0.65 +0.9 372,013 - CA265_RS01455 0.67 +0.3 372,021 - CA265_RS01455 0.68 +1.2 372,023 + CA265_RS01455 0.68 +0.1 372,049 - CA265_RS01455 0.70 +0.1 372,052 + CA265_RS01455 0.70 +1.3 372,053 - CA265_RS01455 0.71 +0.9 372,053 - CA265_RS01455 0.71 -0.6 372,085 - CA265_RS01455 0.73 +1.0 372,085 - CA265_RS01455 0.73 -0.1 372,085 - CA265_RS01455 0.73 -1.0 372,125 + CA265_RS01455 0.76 -2.8 372,125 + CA265_RS01455 0.76 +0.1 372,144 + CA265_RS01455 0.78 -2.3
Or see this region's nucleotide sequence