Experiment: KB with Erythromycin 25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2167 and Psyr_2168 are separated by 3 nucleotides Psyr_2168 and Psyr_2169 overlap by 20 nucleotides Psyr_2169 and Psyr_RNA40 are separated by 88 nucleotides Psyr_RNA40 and Psyr_2170 are separated by 158 nucleotides
Psyr_2167: Psyr_2167 - phenylalanyl-tRNA synthetase beta subunit, at 2,519,998 to 2,522,376
_2167
Psyr_2168: Psyr_2168 - Integration host factor, alpha subunit, at 2,522,380 to 2,522,682
_2168
Psyr_2169: Psyr_2169 - transcriptional regulator, MerR family, at 2,522,663 to 2,523,019
_2169
Psyr_RNA40: Psyr_RNA40 - tRNA-Pro, at 2,523,108 to 2,523,184
_RNA40
Psyr_2170: Psyr_2170 - L-glutamate-binding protein / L-aspartate-binding protein, at 2,523,343 to 2,524,257
_2170
Position (kb)
2522
2523
2524 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2522.374 kb on + strand at 2522.419 kb on + strand, within Psyr_2168 at 2522.439 kb on + strand, within Psyr_2168 at 2522.503 kb on - strand, within Psyr_2168 at 2522.662 kb on + strand at 2522.662 kb on + strand at 2522.662 kb on + strand at 2522.662 kb on + strand at 2522.663 kb on - strand at 2522.663 kb on - strand at 2522.680 kb on + strand at 2522.680 kb on + strand at 2522.714 kb on + strand, within Psyr_2169 at 2522.714 kb on + strand, within Psyr_2169 at 2522.715 kb on - strand, within Psyr_2169 at 2522.715 kb on - strand, within Psyr_2169 at 2522.771 kb on + strand, within Psyr_2169 at 2522.877 kb on + strand, within Psyr_2169 at 2522.877 kb on + strand, within Psyr_2169 at 2522.878 kb on - strand, within Psyr_2169 at 2522.879 kb on + strand at 2522.880 kb on - strand, within Psyr_2169 at 2522.880 kb on - strand, within Psyr_2169 at 2522.954 kb on + strand, within Psyr_2169 at 2522.954 kb on + strand, within Psyr_2169 at 2522.955 kb on - strand, within Psyr_2169 at 2522.955 kb on - strand, within Psyr_2169 at 2522.955 kb on - strand, within Psyr_2169 at 2522.997 kb on + strand at 2522.997 kb on + strand at 2522.998 kb on - strand at 2522.998 kb on - strand at 2522.998 kb on - strand at 2523.063 kb on - strand at 2523.096 kb on + strand at 2523.096 kb on + strand at 2523.097 kb on - strand at 2523.186 kb on - strand at 2523.187 kb on + strand at 2523.188 kb on - strand at 2523.188 kb on - strand at 2523.288 kb on - strand at 2523.454 kb on - strand, within Psyr_2170 at 2523.463 kb on - strand, within Psyr_2170 at 2523.467 kb on + strand, within Psyr_2170 at 2523.468 kb on - strand, within Psyr_2170 at 2523.504 kb on + strand, within Psyr_2170 at 2523.505 kb on - strand, within Psyr_2170 at 2523.505 kb on - strand, within Psyr_2170 at 2523.505 kb on - strand, within Psyr_2170 at 2523.563 kb on + strand, within Psyr_2170 at 2523.563 kb on + strand, within Psyr_2170 at 2523.564 kb on - strand, within Psyr_2170 at 2523.657 kb on + strand, within Psyr_2170 at 2523.658 kb on - strand, within Psyr_2170 at 2523.658 kb on - strand, within Psyr_2170 at 2523.692 kb on + strand, within Psyr_2170 at 2523.693 kb on - strand, within Psyr_2170 at 2523.721 kb on + strand, within Psyr_2170 at 2523.721 kb on + strand, within Psyr_2170 at 2523.721 kb on + strand, within Psyr_2170 at 2523.790 kb on - strand, within Psyr_2170 at 2523.797 kb on - strand, within Psyr_2170 at 2523.831 kb on - strand, within Psyr_2170 at 2523.831 kb on - strand, within Psyr_2170 at 2523.995 kb on + strand, within Psyr_2170 at 2523.995 kb on + strand, within Psyr_2170 at 2523.996 kb on - strand, within Psyr_2170 at 2523.996 kb on - strand, within Psyr_2170 at 2524.000 kb on + strand, within Psyr_2170 at 2524.000 kb on + strand, within Psyr_2170 at 2524.000 kb on + strand, within Psyr_2170 at 2524.001 kb on - strand, within Psyr_2170
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Erythromycin 25 ug/ml remove 2,522,374 + +2.6 2,522,419 + Psyr_2168 0.13 -0.8 2,522,439 + Psyr_2168 0.19 +1.5 2,522,503 - Psyr_2168 0.41 +0.0 2,522,662 + -0.2 2,522,662 + -2.0 2,522,662 + -0.1 2,522,662 + +0.0 2,522,663 - +0.0 2,522,663 - +1.2 2,522,680 + -0.1 2,522,680 + +1.2 2,522,714 + Psyr_2169 0.14 -1.4 2,522,714 + Psyr_2169 0.14 -1.6 2,522,715 - Psyr_2169 0.15 -1.5 2,522,715 - Psyr_2169 0.15 -0.5 2,522,771 + Psyr_2169 0.30 +1.0 2,522,877 + Psyr_2169 0.60 -1.2 2,522,877 + Psyr_2169 0.60 -1.1 2,522,878 - Psyr_2169 0.60 -1.6 2,522,879 + +1.4 2,522,880 - Psyr_2169 0.61 -1.5 2,522,880 - Psyr_2169 0.61 +0.1 2,522,954 + Psyr_2169 0.82 -0.2 2,522,954 + Psyr_2169 0.82 +1.6 2,522,955 - Psyr_2169 0.82 -1.6 2,522,955 - Psyr_2169 0.82 -0.6 2,522,955 - Psyr_2169 0.82 -0.1 2,522,997 + +0.8 2,522,997 + +0.8 2,522,998 - +0.1 2,522,998 - -2.0 2,522,998 - -0.5 2,523,063 - -0.7 2,523,096 + +0.2 2,523,096 + +0.2 2,523,097 - -1.4 2,523,186 - +1.4 2,523,187 + -0.2 2,523,188 - -1.2 2,523,188 - -0.2 2,523,288 - -0.6 2,523,454 - Psyr_2170 0.12 -3.0 2,523,463 - Psyr_2170 0.13 -0.4 2,523,467 + Psyr_2170 0.14 -2.2 2,523,468 - Psyr_2170 0.14 +1.5 2,523,504 + Psyr_2170 0.18 -2.0 2,523,505 - Psyr_2170 0.18 +1.8 2,523,505 - Psyr_2170 0.18 -1.9 2,523,505 - Psyr_2170 0.18 -1.2 2,523,563 + Psyr_2170 0.24 -0.9 2,523,563 + Psyr_2170 0.24 +0.3 2,523,564 - Psyr_2170 0.24 -1.0 2,523,657 + Psyr_2170 0.34 +0.3 2,523,658 - Psyr_2170 0.34 -2.4 2,523,658 - Psyr_2170 0.34 +0.8 2,523,692 + Psyr_2170 0.38 +0.2 2,523,693 - Psyr_2170 0.38 -1.7 2,523,721 + Psyr_2170 0.41 +0.3 2,523,721 + Psyr_2170 0.41 -1.2 2,523,721 + Psyr_2170 0.41 -1.2 2,523,790 - Psyr_2170 0.49 +0.4 2,523,797 - Psyr_2170 0.50 -0.2 2,523,831 - Psyr_2170 0.53 -0.8 2,523,831 - Psyr_2170 0.53 +0.5 2,523,995 + Psyr_2170 0.71 +0.8 2,523,995 + Psyr_2170 0.71 -1.2 2,523,996 - Psyr_2170 0.71 -0.4 2,523,996 - Psyr_2170 0.71 -0.8 2,524,000 + Psyr_2170 0.72 +0.8 2,524,000 + Psyr_2170 0.72 +0.8 2,524,000 + Psyr_2170 0.72 +0.8 2,524,001 - Psyr_2170 0.72 -0.8
Or see this region's nucleotide sequence