Experiment: KB with Erythromycin 25 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1858 and Psyr_1859 are separated by 110 nucleotides Psyr_1859 and Psyr_1860 are separated by 346 nucleotides Psyr_1860 and Psyr_1861 are separated by 100 nucleotides
Psyr_1858: Psyr_1858 - transcriptional regulator, LuxR family, at 2,135,456 to 2,136,178
_1858
Psyr_1859: Psyr_1859 - conserved hypothetical protein, at 2,136,289 to 2,136,567
_1859
Psyr_1860: Psyr_1860 - Ankyrin, at 2,136,914 to 2,137,459
_1860
Psyr_1861: Psyr_1861 - conserved hypothetical protein, at 2,137,560 to 2,138,078
_1861
Position (kb)
2136
2137 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2135.373 kb on + strand at 2135.373 kb on + strand at 2135.382 kb on - strand at 2135.382 kb on - strand at 2135.382 kb on - strand at 2135.493 kb on + strand at 2135.493 kb on + strand at 2135.493 kb on + strand at 2135.493 kb on + strand at 2135.494 kb on - strand at 2135.494 kb on - strand at 2135.494 kb on - strand at 2135.673 kb on - strand, within Psyr_1858 at 2135.783 kb on + strand, within Psyr_1858 at 2135.783 kb on + strand, within Psyr_1858 at 2135.783 kb on + strand, within Psyr_1858 at 2135.783 kb on + strand, within Psyr_1858 at 2135.784 kb on - strand, within Psyr_1858 at 2135.828 kb on + strand, within Psyr_1858 at 2135.828 kb on + strand, within Psyr_1858 at 2135.829 kb on - strand, within Psyr_1858 at 2135.834 kb on + strand, within Psyr_1858 at 2135.834 kb on + strand, within Psyr_1858 at 2135.836 kb on + strand, within Psyr_1858 at 2135.878 kb on + strand, within Psyr_1858 at 2135.878 kb on + strand, within Psyr_1858 at 2135.921 kb on + strand, within Psyr_1858 at 2135.921 kb on + strand, within Psyr_1858 at 2136.089 kb on + strand, within Psyr_1858 at 2136.089 kb on + strand, within Psyr_1858 at 2136.089 kb on + strand, within Psyr_1858 at 2136.090 kb on - strand, within Psyr_1858 at 2136.090 kb on - strand, within Psyr_1858 at 2136.107 kb on + strand at 2136.108 kb on - strand at 2136.255 kb on - strand at 2136.302 kb on + strand at 2136.302 kb on + strand at 2136.434 kb on + strand, within Psyr_1859 at 2136.434 kb on + strand, within Psyr_1859 at 2136.435 kb on - strand, within Psyr_1859 at 2136.460 kb on - strand, within Psyr_1859 at 2136.524 kb on + strand, within Psyr_1859 at 2136.559 kb on - strand at 2136.609 kb on + strand at 2136.609 kb on + strand at 2136.610 kb on - strand at 2136.612 kb on + strand at 2136.613 kb on - strand at 2136.613 kb on - strand at 2136.908 kb on + strand at 2136.908 kb on + strand at 2136.908 kb on + strand at 2136.909 kb on - strand at 2136.909 kb on - strand at 2136.912 kb on + strand at 2136.913 kb on - strand at 2136.923 kb on - strand at 2137.097 kb on + strand, within Psyr_1860 at 2137.097 kb on + strand, within Psyr_1860 at 2137.097 kb on + strand, within Psyr_1860 at 2137.097 kb on + strand, within Psyr_1860 at 2137.098 kb on - strand, within Psyr_1860 at 2137.098 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.249 kb on - strand, within Psyr_1860 at 2137.314 kb on + strand, within Psyr_1860 at 2137.315 kb on - strand, within Psyr_1860 at 2137.493 kb on + strand at 2137.494 kb on - strand at 2137.494 kb on - strand at 2137.494 kb on - strand at 2137.494 kb on - strand at 2137.511 kb on - strand at 2137.511 kb on - strand at 2137.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Erythromycin 25 ug/ml remove 2,135,373 + -1.0 2,135,373 + -0.2 2,135,382 - -0.5 2,135,382 - -0.2 2,135,382 - -1.3 2,135,493 + +0.8 2,135,493 + -0.6 2,135,493 + +1.5 2,135,493 + +0.7 2,135,494 - -2.9 2,135,494 - -1.2 2,135,494 - +0.7 2,135,673 - Psyr_1858 0.30 +0.4 2,135,783 + Psyr_1858 0.45 +1.1 2,135,783 + Psyr_1858 0.45 +3.4 2,135,783 + Psyr_1858 0.45 +0.1 2,135,783 + Psyr_1858 0.45 -0.4 2,135,784 - Psyr_1858 0.45 -0.3 2,135,828 + Psyr_1858 0.51 -1.2 2,135,828 + Psyr_1858 0.51 -0.4 2,135,829 - Psyr_1858 0.52 -0.2 2,135,834 + Psyr_1858 0.52 -1.0 2,135,834 + Psyr_1858 0.52 +0.8 2,135,836 + Psyr_1858 0.53 -2.6 2,135,878 + Psyr_1858 0.58 +1.1 2,135,878 + Psyr_1858 0.58 +0.5 2,135,921 + Psyr_1858 0.64 +2.4 2,135,921 + Psyr_1858 0.64 -0.3 2,136,089 + Psyr_1858 0.88 -0.3 2,136,089 + Psyr_1858 0.88 +0.8 2,136,089 + Psyr_1858 0.88 +1.9 2,136,090 - Psyr_1858 0.88 +2.2 2,136,090 - Psyr_1858 0.88 -0.5 2,136,107 + -1.8 2,136,108 - -0.8 2,136,255 - -0.2 2,136,302 + -0.5 2,136,302 + +0.3 2,136,434 + Psyr_1859 0.52 -0.0 2,136,434 + Psyr_1859 0.52 +0.3 2,136,435 - Psyr_1859 0.52 +1.6 2,136,460 - Psyr_1859 0.61 +1.0 2,136,524 + Psyr_1859 0.84 +1.1 2,136,559 - -1.0 2,136,609 + -0.9 2,136,609 + +0.6 2,136,610 - +1.8 2,136,612 + -0.8 2,136,613 - +0.6 2,136,613 - +1.2 2,136,908 + -0.1 2,136,908 + -0.2 2,136,908 + -1.2 2,136,909 - -0.5 2,136,909 - +0.3 2,136,912 + -1.5 2,136,913 - -1.1 2,136,923 - -1.2 2,137,097 + Psyr_1860 0.34 -1.5 2,137,097 + Psyr_1860 0.34 +0.3 2,137,097 + Psyr_1860 0.34 +0.8 2,137,097 + Psyr_1860 0.34 -0.9 2,137,098 - Psyr_1860 0.34 -1.8 2,137,098 - Psyr_1860 0.34 -2.8 2,137,249 - Psyr_1860 0.61 +0.2 2,137,249 - Psyr_1860 0.61 -0.4 2,137,249 - Psyr_1860 0.61 +1.0 2,137,249 - Psyr_1860 0.61 -1.5 2,137,249 - Psyr_1860 0.61 +1.7 2,137,249 - Psyr_1860 0.61 -0.7 2,137,249 - Psyr_1860 0.61 +0.1 2,137,314 + Psyr_1860 0.73 -0.2 2,137,315 - Psyr_1860 0.73 -1.6 2,137,493 + +0.7 2,137,494 - -0.8 2,137,494 - +0.5 2,137,494 - +0.3 2,137,494 - +0.1 2,137,511 - -0.6 2,137,511 - +0.7 2,137,511 - +0.8
Or see this region's nucleotide sequence