Experiment: D-Glucose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3984 and Pf1N1B4_3985 overlap by 1 nucleotides Pf1N1B4_3985 and Pf1N1B4_3986 are separated by 115 nucleotides Pf1N1B4_3986 and Pf1N1B4_3987 are separated by 12 nucleotides
Pf1N1B4_3984 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 4,424,874 to 4,426,829
_3984
Pf1N1B4_3985 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18), at 4,426,829 to 4,427,644
_3985
Pf1N1B4_3986 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 4,427,760 to 4,429,367
_3986
Pf1N1B4_3987 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 4,429,380 to 4,430,543
_3987
Position (kb)
4427
4428
4429
4430 Strain fitness (log2 ratio)
-2
-1
0
1 at 4427.321 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985 at 4427.891 kb on - strand at 4427.891 kb on - strand at 4427.891 kb on - strand at 4427.891 kb on - strand at 4427.894 kb on - strand at 4428.104 kb on - strand, within Pf1N1B4_3986 at 4428.215 kb on - strand, within Pf1N1B4_3986 at 4428.392 kb on + strand, within Pf1N1B4_3986 at 4428.783 kb on + strand, within Pf1N1B4_3986 at 4428.787 kb on + strand, within Pf1N1B4_3986 at 4428.788 kb on - strand, within Pf1N1B4_3986 at 4428.788 kb on - strand, within Pf1N1B4_3986 at 4428.946 kb on + strand, within Pf1N1B4_3986 at 4428.947 kb on - strand, within Pf1N1B4_3986 at 4428.947 kb on - strand, within Pf1N1B4_3986 at 4428.947 kb on - strand, within Pf1N1B4_3986 at 4429.018 kb on + strand, within Pf1N1B4_3986 at 4429.018 kb on + strand, within Pf1N1B4_3986 at 4429.018 kb on + strand, within Pf1N1B4_3986 at 4429.018 kb on + strand, within Pf1N1B4_3986 at 4429.019 kb on - strand, within Pf1N1B4_3986 at 4429.223 kb on + strand at 4429.224 kb on - strand at 4429.224 kb on - strand at 4429.224 kb on - strand at 4429.224 kb on - strand at 4429.224 kb on - strand at 4429.224 kb on - strand at 4429.291 kb on - strand at 4429.454 kb on - strand at 4429.502 kb on - strand, within Pf1N1B4_3987 at 4429.502 kb on - strand, within Pf1N1B4_3987 at 4429.502 kb on - strand, within Pf1N1B4_3987 at 4429.564 kb on + strand, within Pf1N1B4_3987 at 4429.630 kb on + strand, within Pf1N1B4_3987 at 4429.630 kb on + strand, within Pf1N1B4_3987 at 4429.637 kb on - strand, within Pf1N1B4_3987 at 4429.663 kb on + strand, within Pf1N1B4_3987 at 4429.663 kb on + strand, within Pf1N1B4_3987 at 4429.664 kb on - strand, within Pf1N1B4_3987 at 4429.664 kb on - strand, within Pf1N1B4_3987 at 4429.664 kb on - strand at 4429.738 kb on + strand, within Pf1N1B4_3987 at 4429.827 kb on + strand, within Pf1N1B4_3987 at 4429.828 kb on - strand, within Pf1N1B4_3987 at 4430.023 kb on + strand, within Pf1N1B4_3987 at 4430.023 kb on + strand, within Pf1N1B4_3987 at 4430.024 kb on - strand at 4430.032 kb on + strand, within Pf1N1B4_3987 at 4430.032 kb on + strand, within Pf1N1B4_3987 at 4430.033 kb on - strand, within Pf1N1B4_3987 at 4430.082 kb on + strand, within Pf1N1B4_3987 at 4430.083 kb on - strand, within Pf1N1B4_3987 at 4430.086 kb on + strand, within Pf1N1B4_3987 at 4430.102 kb on + strand, within Pf1N1B4_3987 at 4430.200 kb on + strand, within Pf1N1B4_3987 at 4430.201 kb on - strand, within Pf1N1B4_3987 at 4430.201 kb on - strand, within Pf1N1B4_3987 at 4430.353 kb on + strand, within Pf1N1B4_3987
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) remove 4,427,321 + Pf1N1B4_3985 0.60 +0.8 4,427,361 + Pf1N1B4_3985 0.65 -0.5 4,427,361 + Pf1N1B4_3985 0.65 +0.9 4,427,361 + Pf1N1B4_3985 0.65 -1.3 4,427,891 - +0.4 4,427,891 - +1.0 4,427,891 - +0.3 4,427,891 - +0.8 4,427,894 - +0.4 4,428,104 - Pf1N1B4_3986 0.21 -1.6 4,428,215 - Pf1N1B4_3986 0.28 -1.0 4,428,392 + Pf1N1B4_3986 0.39 -0.6 4,428,783 + Pf1N1B4_3986 0.64 -1.0 4,428,787 + Pf1N1B4_3986 0.64 -1.2 4,428,788 - Pf1N1B4_3986 0.64 -1.4 4,428,788 - Pf1N1B4_3986 0.64 -1.4 4,428,946 + Pf1N1B4_3986 0.74 +0.2 4,428,947 - Pf1N1B4_3986 0.74 -2.5 4,428,947 - Pf1N1B4_3986 0.74 -2.6 4,428,947 - Pf1N1B4_3986 0.74 -0.0 4,429,018 + Pf1N1B4_3986 0.78 +0.3 4,429,018 + Pf1N1B4_3986 0.78 +0.8 4,429,018 + Pf1N1B4_3986 0.78 -0.0 4,429,018 + Pf1N1B4_3986 0.78 -0.4 4,429,019 - Pf1N1B4_3986 0.78 +1.0 4,429,223 + -0.6 4,429,224 - -0.0 4,429,224 - -0.2 4,429,224 - +0.5 4,429,224 - -0.8 4,429,224 - -0.0 4,429,224 - -0.5 4,429,291 - +0.1 4,429,454 - -0.2 4,429,502 - Pf1N1B4_3987 0.10 +0.4 4,429,502 - Pf1N1B4_3987 0.10 -0.3 4,429,502 - Pf1N1B4_3987 0.10 -0.6 4,429,564 + Pf1N1B4_3987 0.16 -0.0 4,429,630 + Pf1N1B4_3987 0.21 -0.2 4,429,630 + Pf1N1B4_3987 0.21 -0.3 4,429,637 - Pf1N1B4_3987 0.22 +0.4 4,429,663 + Pf1N1B4_3987 0.24 +0.2 4,429,663 + Pf1N1B4_3987 0.24 -0.3 4,429,664 - Pf1N1B4_3987 0.24 -1.8 4,429,664 - Pf1N1B4_3987 0.24 -0.3 4,429,664 - +0.7 4,429,738 + Pf1N1B4_3987 0.31 +0.4 4,429,827 + Pf1N1B4_3987 0.38 -0.9 4,429,828 - Pf1N1B4_3987 0.38 -0.6 4,430,023 + Pf1N1B4_3987 0.55 -0.4 4,430,023 + Pf1N1B4_3987 0.55 -1.3 4,430,024 - +1.0 4,430,032 + Pf1N1B4_3987 0.56 +0.3 4,430,032 + Pf1N1B4_3987 0.56 +0.4 4,430,033 - Pf1N1B4_3987 0.56 +1.0 4,430,082 + Pf1N1B4_3987 0.60 -0.2 4,430,083 - Pf1N1B4_3987 0.60 +0.1 4,430,086 + Pf1N1B4_3987 0.61 +0.4 4,430,102 + Pf1N1B4_3987 0.62 -0.2 4,430,200 + Pf1N1B4_3987 0.70 +1.0 4,430,201 - Pf1N1B4_3987 0.71 -0.0 4,430,201 - Pf1N1B4_3987 0.71 -1.0 4,430,353 + Pf1N1B4_3987 0.84 -0.3
Or see this region's nucleotide sequence