Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3986

Experiment: D-Glucose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3984 and Pf1N1B4_3985 overlap by 1 nucleotidesPf1N1B4_3985 and Pf1N1B4_3986 are separated by 115 nucleotidesPf1N1B4_3986 and Pf1N1B4_3987 are separated by 12 nucleotides Pf1N1B4_3984 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data), at 4,424,874 to 4,426,829 _3984 Pf1N1B4_3985 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18), at 4,426,829 to 4,427,644 _3985 Pf1N1B4_3986 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 4,427,760 to 4,429,367 _3986 Pf1N1B4_3987 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 4,429,380 to 4,430,543 _3987 Position (kb) 4427 4428 4429 4430Strain fitness (log2 ratio) -2 -1 0 1at 4427.321 kb on + strand, within Pf1N1B4_3985at 4427.361 kb on + strand, within Pf1N1B4_3985at 4427.361 kb on + strand, within Pf1N1B4_3985at 4427.361 kb on + strand, within Pf1N1B4_3985at 4427.891 kb on - strandat 4427.891 kb on - strandat 4427.891 kb on - strandat 4427.891 kb on - strandat 4427.894 kb on - strandat 4428.104 kb on - strand, within Pf1N1B4_3986at 4428.215 kb on - strand, within Pf1N1B4_3986at 4428.392 kb on + strand, within Pf1N1B4_3986at 4428.783 kb on + strand, within Pf1N1B4_3986at 4428.787 kb on + strand, within Pf1N1B4_3986at 4428.788 kb on - strand, within Pf1N1B4_3986at 4428.788 kb on - strand, within Pf1N1B4_3986at 4428.946 kb on + strand, within Pf1N1B4_3986at 4428.947 kb on - strand, within Pf1N1B4_3986at 4428.947 kb on - strand, within Pf1N1B4_3986at 4428.947 kb on - strand, within Pf1N1B4_3986at 4429.018 kb on + strand, within Pf1N1B4_3986at 4429.018 kb on + strand, within Pf1N1B4_3986at 4429.018 kb on + strand, within Pf1N1B4_3986at 4429.018 kb on + strand, within Pf1N1B4_3986at 4429.019 kb on - strand, within Pf1N1B4_3986at 4429.223 kb on + strandat 4429.224 kb on - strandat 4429.224 kb on - strandat 4429.224 kb on - strandat 4429.224 kb on - strandat 4429.224 kb on - strandat 4429.224 kb on - strandat 4429.291 kb on - strandat 4429.454 kb on - strandat 4429.502 kb on - strand, within Pf1N1B4_3987at 4429.502 kb on - strand, within Pf1N1B4_3987at 4429.502 kb on - strand, within Pf1N1B4_3987at 4429.564 kb on + strand, within Pf1N1B4_3987at 4429.630 kb on + strand, within Pf1N1B4_3987at 4429.630 kb on + strand, within Pf1N1B4_3987at 4429.637 kb on - strand, within Pf1N1B4_3987at 4429.663 kb on + strand, within Pf1N1B4_3987at 4429.663 kb on + strand, within Pf1N1B4_3987at 4429.664 kb on - strand, within Pf1N1B4_3987at 4429.664 kb on - strand, within Pf1N1B4_3987at 4429.664 kb on - strandat 4429.738 kb on + strand, within Pf1N1B4_3987at 4429.827 kb on + strand, within Pf1N1B4_3987at 4429.828 kb on - strand, within Pf1N1B4_3987at 4430.023 kb on + strand, within Pf1N1B4_3987at 4430.023 kb on + strand, within Pf1N1B4_3987at 4430.024 kb on - strandat 4430.032 kb on + strand, within Pf1N1B4_3987at 4430.032 kb on + strand, within Pf1N1B4_3987at 4430.033 kb on - strand, within Pf1N1B4_3987at 4430.082 kb on + strand, within Pf1N1B4_3987at 4430.083 kb on - strand, within Pf1N1B4_3987at 4430.086 kb on + strand, within Pf1N1B4_3987at 4430.102 kb on + strand, within Pf1N1B4_3987at 4430.200 kb on + strand, within Pf1N1B4_3987at 4430.201 kb on - strand, within Pf1N1B4_3987at 4430.201 kb on - strand, within Pf1N1B4_3987at 4430.353 kb on + strand, within Pf1N1B4_3987

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C)
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4,427,321 + Pf1N1B4_3985 0.60 +0.8
4,427,361 + Pf1N1B4_3985 0.65 -0.5
4,427,361 + Pf1N1B4_3985 0.65 +0.9
4,427,361 + Pf1N1B4_3985 0.65 -1.3
4,427,891 - +0.4
4,427,891 - +1.0
4,427,891 - +0.3
4,427,891 - +0.8
4,427,894 - +0.4
4,428,104 - Pf1N1B4_3986 0.21 -1.6
4,428,215 - Pf1N1B4_3986 0.28 -1.0
4,428,392 + Pf1N1B4_3986 0.39 -0.6
4,428,783 + Pf1N1B4_3986 0.64 -1.0
4,428,787 + Pf1N1B4_3986 0.64 -1.2
4,428,788 - Pf1N1B4_3986 0.64 -1.4
4,428,788 - Pf1N1B4_3986 0.64 -1.4
4,428,946 + Pf1N1B4_3986 0.74 +0.2
4,428,947 - Pf1N1B4_3986 0.74 -2.5
4,428,947 - Pf1N1B4_3986 0.74 -2.6
4,428,947 - Pf1N1B4_3986 0.74 -0.0
4,429,018 + Pf1N1B4_3986 0.78 +0.3
4,429,018 + Pf1N1B4_3986 0.78 +0.8
4,429,018 + Pf1N1B4_3986 0.78 -0.0
4,429,018 + Pf1N1B4_3986 0.78 -0.4
4,429,019 - Pf1N1B4_3986 0.78 +1.0
4,429,223 + -0.6
4,429,224 - -0.0
4,429,224 - -0.2
4,429,224 - +0.5
4,429,224 - -0.8
4,429,224 - -0.0
4,429,224 - -0.5
4,429,291 - +0.1
4,429,454 - -0.2
4,429,502 - Pf1N1B4_3987 0.10 +0.4
4,429,502 - Pf1N1B4_3987 0.10 -0.3
4,429,502 - Pf1N1B4_3987 0.10 -0.6
4,429,564 + Pf1N1B4_3987 0.16 -0.0
4,429,630 + Pf1N1B4_3987 0.21 -0.2
4,429,630 + Pf1N1B4_3987 0.21 -0.3
4,429,637 - Pf1N1B4_3987 0.22 +0.4
4,429,663 + Pf1N1B4_3987 0.24 +0.2
4,429,663 + Pf1N1B4_3987 0.24 -0.3
4,429,664 - Pf1N1B4_3987 0.24 -1.8
4,429,664 - Pf1N1B4_3987 0.24 -0.3
4,429,664 - +0.7
4,429,738 + Pf1N1B4_3987 0.31 +0.4
4,429,827 + Pf1N1B4_3987 0.38 -0.9
4,429,828 - Pf1N1B4_3987 0.38 -0.6
4,430,023 + Pf1N1B4_3987 0.55 -0.4
4,430,023 + Pf1N1B4_3987 0.55 -1.3
4,430,024 - +1.0
4,430,032 + Pf1N1B4_3987 0.56 +0.3
4,430,032 + Pf1N1B4_3987 0.56 +0.4
4,430,033 - Pf1N1B4_3987 0.56 +1.0
4,430,082 + Pf1N1B4_3987 0.60 -0.2
4,430,083 - Pf1N1B4_3987 0.60 +0.1
4,430,086 + Pf1N1B4_3987 0.61 +0.4
4,430,102 + Pf1N1B4_3987 0.62 -0.2
4,430,200 + Pf1N1B4_3987 0.70 +1.0
4,430,201 - Pf1N1B4_3987 0.71 -0.0
4,430,201 - Pf1N1B4_3987 0.71 -1.0
4,430,353 + Pf1N1B4_3987 0.84 -0.3

Or see this region's nucleotide sequence