Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_328

Experiment: D-Glucose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_327 and Pf1N1B4_328 are separated by 905 nucleotidesPf1N1B4_328 and Pf1N1B4_329 are separated by 531 nucleotides Pf1N1B4_327 - hypothetical protein, at 385,506 to 386,450 _327 Pf1N1B4_328 - Glucosyltransferase (side chain biosynthesis) (EC 2.4.1.-), at 387,356 to 388,624 _328 Pf1N1B4_329 - Acyltransferase, at 389,156 to 390,208 _329 Position (kb) 387 388 389Strain fitness (log2 ratio) -1 0 1 2at 386.938 kb on + strandat 386.939 kb on - strandat 386.940 kb on + strandat 387.303 kb on + strandat 387.304 kb on - strandat 387.305 kb on + strandat 387.453 kb on + strandat 387.722 kb on - strand, within Pf1N1B4_328at 387.755 kb on + strand, within Pf1N1B4_328at 387.770 kb on + strand, within Pf1N1B4_328at 387.967 kb on + strand, within Pf1N1B4_328at 387.967 kb on + strand, within Pf1N1B4_328at 387.979 kb on - strand, within Pf1N1B4_328at 388.032 kb on - strand, within Pf1N1B4_328at 388.036 kb on - strand, within Pf1N1B4_328at 388.174 kb on - strand, within Pf1N1B4_328at 388.268 kb on + strand, within Pf1N1B4_328at 388.273 kb on - strand, within Pf1N1B4_328at 388.385 kb on + strand, within Pf1N1B4_328at 388.461 kb on + strand, within Pf1N1B4_328at 388.461 kb on + strand, within Pf1N1B4_328at 388.540 kb on + strandat 388.541 kb on - strandat 388.541 kb on - strandat 388.541 kb on - strandat 388.606 kb on + strandat 388.607 kb on - strandat 388.628 kb on + strandat 388.789 kb on - strandat 388.941 kb on - strandat 388.991 kb on + strandat 389.131 kb on - strandat 389.132 kb on + strandat 389.133 kb on - strandat 389.251 kb on - strandat 389.304 kb on - strand, within Pf1N1B4_329at 389.617 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C)
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386,938 + +0.3
386,939 - +0.5
386,940 + -0.2
387,303 + -0.9
387,304 - +2.7
387,305 + +0.2
387,453 + -1.2
387,722 - Pf1N1B4_328 0.29 +0.4
387,755 + Pf1N1B4_328 0.31 -0.6
387,770 + Pf1N1B4_328 0.33 +0.1
387,967 + Pf1N1B4_328 0.48 -0.9
387,967 + Pf1N1B4_328 0.48 -1.2
387,979 - Pf1N1B4_328 0.49 +1.1
388,032 - Pf1N1B4_328 0.53 -0.7
388,036 - Pf1N1B4_328 0.54 -0.5
388,174 - Pf1N1B4_328 0.64 -1.2
388,268 + Pf1N1B4_328 0.72 -1.5
388,273 - Pf1N1B4_328 0.72 -0.7
388,385 + Pf1N1B4_328 0.81 -0.9
388,461 + Pf1N1B4_328 0.87 +0.8
388,461 + Pf1N1B4_328 0.87 -1.0
388,540 + +0.3
388,541 - -0.2
388,541 - +0.2
388,541 - -0.6
388,606 + -1.6
388,607 - -0.5
388,628 + -0.3
388,789 - -0.6
388,941 - +1.1
388,991 + -0.3
389,131 - -0.0
389,132 + -0.1
389,133 - +0.1
389,251 - -0.5
389,304 - Pf1N1B4_329 0.14 +0.8
389,617 - +1.1

Or see this region's nucleotide sequence