Experiment: D-Glucose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_321 and Pf1N1B4_322 are separated by 206 nucleotides Pf1N1B4_322 and Pf1N1B4_323 are separated by 299 nucleotides
Pf1N1B4_321 - Probable short-chain dehydrogenase, at 377,661 to 378,455
_321
Pf1N1B4_322 - nucleotide sugar epimerase/dehydratase WbpM, at 378,662 to 380,890
_322
Pf1N1B4_323 - Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-), at 381,190 to 382,218
_323
Position (kb)
378
379
380
381 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 377.669 kb on - strand at 377.691 kb on - strand at 377.691 kb on - strand at 377.691 kb on - strand at 377.726 kb on + strand at 377.726 kb on + strand at 377.727 kb on - strand at 377.885 kb on + strand, within Pf1N1B4_321 at 377.886 kb on - strand, within Pf1N1B4_321 at 377.971 kb on + strand at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand, within Pf1N1B4_321 at 377.972 kb on - strand at 377.989 kb on + strand, within Pf1N1B4_321 at 377.990 kb on - strand, within Pf1N1B4_321 at 377.990 kb on - strand, within Pf1N1B4_321 at 377.990 kb on - strand, within Pf1N1B4_321 at 378.039 kb on - strand, within Pf1N1B4_321 at 378.186 kb on - strand, within Pf1N1B4_321 at 378.539 kb on + strand at 378.540 kb on - strand at 378.540 kb on - strand at 378.553 kb on + strand at 378.554 kb on - strand at 378.554 kb on - strand at 378.558 kb on + strand at 378.565 kb on - strand at 378.613 kb on - strand at 378.613 kb on - strand at 378.656 kb on - strand at 378.656 kb on - strand at 378.656 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.664 kb on - strand at 378.700 kb on + strand at 378.701 kb on - strand at 378.944 kb on - strand, within Pf1N1B4_322 at 378.979 kb on - strand, within Pf1N1B4_322 at 378.979 kb on - strand at 378.979 kb on - strand, within Pf1N1B4_322 at 378.998 kb on - strand, within Pf1N1B4_322 at 378.998 kb on - strand, within Pf1N1B4_322 at 379.693 kb on + strand, within Pf1N1B4_322 at 379.948 kb on - strand, within Pf1N1B4_322 at 380.481 kb on + strand at 380.481 kb on + strand, within Pf1N1B4_322 at 380.501 kb on - strand, within Pf1N1B4_322 at 380.742 kb on + strand at 380.918 kb on + strand at 380.967 kb on + strand at 380.968 kb on - strand at 380.968 kb on - strand at 381.040 kb on + strand at 381.160 kb on - strand at 381.242 kb on - strand at 381.260 kb on + strand at 381.260 kb on + strand at 381.395 kb on + strand, within Pf1N1B4_323 at 381.695 kb on + strand, within Pf1N1B4_323
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) remove 377,669 - +0.2 377,691 - -0.5 377,691 - -2.4 377,691 - -0.1 377,726 + +0.3 377,726 + +1.4 377,727 - +0.4 377,885 + Pf1N1B4_321 0.28 -0.3 377,886 - Pf1N1B4_321 0.28 -0.8 377,971 + +0.5 377,972 - Pf1N1B4_321 0.39 -0.1 377,972 - Pf1N1B4_321 0.39 -0.7 377,972 - Pf1N1B4_321 0.39 -0.0 377,972 - Pf1N1B4_321 0.39 -2.5 377,972 - Pf1N1B4_321 0.39 -0.0 377,972 - Pf1N1B4_321 0.39 -0.0 377,972 - Pf1N1B4_321 0.39 +1.7 377,972 - Pf1N1B4_321 0.39 +0.1 377,972 - Pf1N1B4_321 0.39 -0.2 377,972 - Pf1N1B4_321 0.39 +0.7 377,972 - Pf1N1B4_321 0.39 -1.9 377,972 - Pf1N1B4_321 0.39 -0.5 377,972 - Pf1N1B4_321 0.39 +0.5 377,972 - -1.5 377,989 + Pf1N1B4_321 0.41 -1.3 377,990 - Pf1N1B4_321 0.41 -0.1 377,990 - Pf1N1B4_321 0.41 +0.5 377,990 - Pf1N1B4_321 0.41 -0.7 378,039 - Pf1N1B4_321 0.48 +0.1 378,186 - Pf1N1B4_321 0.66 -1.1 378,539 + +0.4 378,540 - +0.6 378,540 - +0.8 378,553 + -1.9 378,554 - +1.1 378,554 - -0.5 378,558 + -2.2 378,565 - -0.2 378,613 - -0.5 378,613 - -0.5 378,656 - +1.1 378,656 - -0.3 378,656 - -0.7 378,664 - -1.5 378,664 - +0.1 378,664 - +0.1 378,664 - +2.1 378,664 - +1.1 378,664 - -0.9 378,664 - -1.1 378,664 - -0.7 378,700 + -1.3 378,701 - -1.5 378,944 - Pf1N1B4_322 0.13 +0.1 378,979 - Pf1N1B4_322 0.14 -1.5 378,979 - +0.7 378,979 - Pf1N1B4_322 0.14 -0.1 378,998 - Pf1N1B4_322 0.15 +0.1 378,998 - Pf1N1B4_322 0.15 +0.1 379,693 + Pf1N1B4_322 0.46 -1.5 379,948 - Pf1N1B4_322 0.58 +0.4 380,481 + +0.1 380,481 + Pf1N1B4_322 0.82 -2.3 380,501 - Pf1N1B4_322 0.83 -0.7 380,742 + +0.4 380,918 + -0.6 380,967 + +0.1 380,968 - -0.9 380,968 - -0.5 381,040 + +0.8 381,160 - -0.6 381,242 - +1.1 381,260 + -0.5 381,260 + -1.3 381,395 + Pf1N1B4_323 0.20 -2.5 381,695 + Pf1N1B4_323 0.49 -1.1
Or see this region's nucleotide sequence