Experiment: R2A with nitrite 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22550 and CA265_RS22555 overlap by 1 nucleotides CA265_RS22555 and CA265_RS22560 are separated by 11 nucleotides CA265_RS22560 and CA265_RS22565 are separated by 71 nucleotides
CA265_RS22550: CA265_RS22550 - hypothetical protein, at 5,525,972 to 5,527,030
_RS22550
CA265_RS22555: CA265_RS22555 - lipoyl(octanoyl) transferase, at 5,527,030 to 5,527,758
_RS22555
CA265_RS22560: CA265_RS22560 - 4'-phosphopantetheinyl transferase, at 5,527,770 to 5,528,417
_RS22560
CA265_RS22565: CA265_RS22565 - dCTP deaminase, at 5,528,489 to 5,529,025
_RS22565
Position (kb) 5527
5528 Strain fitness (log2 ratio) -2
-1
0
1 at 5526.105 kb on - strand, within CA265_RS22550 at 5526.152 kb on - strand, within CA265_RS22550 at 5526.152 kb on - strand, within CA265_RS22550 at 5526.187 kb on + strand, within CA265_RS22550 at 5526.218 kb on - strand, within CA265_RS22550 at 5526.294 kb on - strand, within CA265_RS22550 at 5526.313 kb on + strand, within CA265_RS22550 at 5526.322 kb on + strand, within CA265_RS22550 at 5526.550 kb on + strand, within CA265_RS22550 at 5526.551 kb on - strand, within CA265_RS22550 at 5526.577 kb on + strand, within CA265_RS22550 at 5526.618 kb on + strand, within CA265_RS22550 at 5526.619 kb on - strand, within CA265_RS22550 at 5526.640 kb on + strand, within CA265_RS22550 at 5526.641 kb on - strand, within CA265_RS22550 at 5526.721 kb on + strand, within CA265_RS22550 at 5526.722 kb on - strand, within CA265_RS22550 at 5526.751 kb on - strand, within CA265_RS22550 at 5526.753 kb on + strand, within CA265_RS22550 at 5526.778 kb on + strand, within CA265_RS22550 at 5526.779 kb on - strand, within CA265_RS22550 at 5526.882 kb on + strand, within CA265_RS22550 at 5526.883 kb on - strand, within CA265_RS22550 at 5526.890 kb on + strand, within CA265_RS22550 at 5526.914 kb on + strand, within CA265_RS22550 at 5526.915 kb on - strand, within CA265_RS22550 at 5526.991 kb on + strand at 5526.991 kb on + strand at 5526.991 kb on + strand at 5526.991 kb on + strand at 5527.002 kb on - strand at 5527.031 kb on + strand at 5527.767 kb on - strand at 5527.785 kb on + strand at 5527.785 kb on + strand at 5527.795 kb on - strand at 5528.434 kb on + strand at 5528.455 kb on + strand at 5528.463 kb on - strand at 5528.569 kb on - strand, within CA265_RS22565 at 5528.603 kb on + strand, within CA265_RS22565 at 5528.611 kb on + strand, within CA265_RS22565 at 5528.617 kb on + strand, within CA265_RS22565 at 5528.618 kb on - strand, within CA265_RS22565 at 5528.675 kb on - strand, within CA265_RS22565 at 5528.685 kb on - strand, within CA265_RS22565 at 5528.685 kb on - strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.687 kb on - strand, within CA265_RS22565 at 5528.707 kb on - strand, within CA265_RS22565 at 5528.724 kb on + strand, within CA265_RS22565 at 5528.729 kb on + strand, within CA265_RS22565 at 5528.751 kb on + strand, within CA265_RS22565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 3 mM remove 5,526,105 - CA265_RS22550 0.13 -1.3 5,526,152 - CA265_RS22550 0.17 +0.3 5,526,152 - CA265_RS22550 0.17 +0.4 5,526,187 + CA265_RS22550 0.20 -0.4 5,526,218 - CA265_RS22550 0.23 +0.8 5,526,294 - CA265_RS22550 0.30 -0.4 5,526,313 + CA265_RS22550 0.32 -0.3 5,526,322 + CA265_RS22550 0.33 -0.3 5,526,550 + CA265_RS22550 0.55 -2.2 5,526,551 - CA265_RS22550 0.55 +0.3 5,526,577 + CA265_RS22550 0.57 +0.1 5,526,618 + CA265_RS22550 0.61 +0.8 5,526,619 - CA265_RS22550 0.61 +0.5 5,526,640 + CA265_RS22550 0.63 +0.5 5,526,641 - CA265_RS22550 0.63 +0.3 5,526,721 + CA265_RS22550 0.71 -0.0 5,526,722 - CA265_RS22550 0.71 -1.0 5,526,751 - CA265_RS22550 0.74 -0.7 5,526,753 + CA265_RS22550 0.74 -0.0 5,526,778 + CA265_RS22550 0.76 -0.2 5,526,779 - CA265_RS22550 0.76 +0.3 5,526,882 + CA265_RS22550 0.86 +0.9 5,526,883 - CA265_RS22550 0.86 -0.7 5,526,890 + CA265_RS22550 0.87 +0.2 5,526,914 + CA265_RS22550 0.89 -0.1 5,526,915 - CA265_RS22550 0.89 +0.1 5,526,991 + +0.4 5,526,991 + +0.1 5,526,991 + +0.2 5,526,991 + +0.2 5,527,002 - -0.7 5,527,031 + +0.3 5,527,767 - +0.2 5,527,785 + +0.4 5,527,785 + -0.0 5,527,795 - -1.7 5,528,434 + -0.5 5,528,455 + +0.7 5,528,463 - +0.0 5,528,569 - CA265_RS22565 0.15 -0.9 5,528,603 + CA265_RS22565 0.21 +1.0 5,528,611 + CA265_RS22565 0.23 -0.7 5,528,617 + CA265_RS22565 0.24 -0.1 5,528,618 - CA265_RS22565 0.24 +0.6 5,528,675 - CA265_RS22565 0.35 -1.7 5,528,685 - CA265_RS22565 0.36 -0.8 5,528,685 - CA265_RS22565 0.36 -0.5 5,528,686 + CA265_RS22565 0.37 -0.7 5,528,686 + CA265_RS22565 0.37 -1.4 5,528,686 + CA265_RS22565 0.37 -0.1 5,528,686 + CA265_RS22565 0.37 -0.2 5,528,687 - CA265_RS22565 0.37 -0.9 5,528,707 - CA265_RS22565 0.41 +0.5 5,528,724 + CA265_RS22565 0.44 -0.7 5,528,729 + CA265_RS22565 0.45 +0.4 5,528,751 + CA265_RS22565 0.49 -1.1
Or see this region's nucleotide sequence