Experiment: R2A with nitrite 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00755 and CA265_RS00760 are separated by 71 nucleotides CA265_RS00760 and CA265_RS00765 are separated by 128 nucleotides CA265_RS00765 and CA265_RS00770 are separated by 32 nucleotides CA265_RS00770 and CA265_RS00775 are separated by 120 nucleotides
CA265_RS00755: CA265_RS00755 - 5-dehydro-4-deoxy-D-glucuronate isomerase, at 182,918 to 183,757
_RS00755
CA265_RS00760: CA265_RS00760 - 2-deoxy-D-gluconate 3-dehydrogenase, at 183,829 to 184,593
_RS00760
CA265_RS00765: CA265_RS00765 - hypothetical protein, at 184,722 to 185,567
_RS00765
CA265_RS00770: CA265_RS00770 - hypothetical protein, at 185,600 to 186,004
_RS00770
CA265_RS00775: CA265_RS00775 - carbonic anhydrase, at 186,125 to 186,751
_RS00775
Position (kb) 184
185
186 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 183.856 kb on - strand at 183.908 kb on + strand, within CA265_RS00760 at 183.909 kb on - strand, within CA265_RS00760 at 184.156 kb on + strand, within CA265_RS00760 at 184.157 kb on - strand, within CA265_RS00760 at 184.198 kb on - strand, within CA265_RS00760 at 184.269 kb on - strand, within CA265_RS00760 at 184.269 kb on - strand, within CA265_RS00760 at 184.379 kb on + strand, within CA265_RS00760 at 184.399 kb on + strand, within CA265_RS00760 at 184.400 kb on - strand, within CA265_RS00760 at 184.402 kb on - strand, within CA265_RS00760 at 184.416 kb on + strand, within CA265_RS00760 at 184.416 kb on + strand, within CA265_RS00760 at 184.455 kb on + strand, within CA265_RS00760 at 184.456 kb on - strand, within CA265_RS00760 at 184.457 kb on + strand, within CA265_RS00760 at 184.457 kb on + strand, within CA265_RS00760 at 184.458 kb on - strand, within CA265_RS00760 at 184.468 kb on - strand, within CA265_RS00760 at 184.518 kb on - strand at 184.543 kb on + strand at 184.591 kb on + strand at 184.592 kb on - strand at 184.592 kb on - strand at 184.680 kb on - strand at 186.085 kb on - strand at 186.113 kb on + strand at 186.114 kb on - strand at 186.114 kb on - strand at 186.126 kb on + strand at 186.127 kb on - strand at 186.129 kb on + strand at 186.139 kb on - strand at 186.284 kb on - strand at 186.304 kb on + strand, within CA265_RS00775 at 186.304 kb on + strand, within CA265_RS00775 at 186.312 kb on - strand, within CA265_RS00775 at 186.324 kb on + strand, within CA265_RS00775 at 186.325 kb on - strand at 186.377 kb on + strand, within CA265_RS00775 at 186.378 kb on - strand, within CA265_RS00775 at 186.411 kb on - strand, within CA265_RS00775 at 186.479 kb on - strand, within CA265_RS00775 at 186.524 kb on + strand, within CA265_RS00775 at 186.524 kb on + strand, within CA265_RS00775 at 186.524 kb on + strand, within CA265_RS00775 at 186.530 kb on + strand, within CA265_RS00775
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 3 mM remove 183,856 - -1.3 183,908 + CA265_RS00760 0.10 -1.2 183,909 - CA265_RS00760 0.10 +0.6 184,156 + CA265_RS00760 0.43 -0.3 184,157 - CA265_RS00760 0.43 -0.2 184,198 - CA265_RS00760 0.48 -0.5 184,269 - CA265_RS00760 0.58 -0.1 184,269 - CA265_RS00760 0.58 -0.3 184,379 + CA265_RS00760 0.72 -0.1 184,399 + CA265_RS00760 0.75 -0.5 184,400 - CA265_RS00760 0.75 -0.7 184,402 - CA265_RS00760 0.75 -2.9 184,416 + CA265_RS00760 0.77 +0.2 184,416 + CA265_RS00760 0.77 -0.2 184,455 + CA265_RS00760 0.82 -0.5 184,456 - CA265_RS00760 0.82 -0.1 184,457 + CA265_RS00760 0.82 +0.0 184,457 + CA265_RS00760 0.82 -0.2 184,458 - CA265_RS00760 0.82 +0.6 184,468 - CA265_RS00760 0.84 +0.3 184,518 - -0.4 184,543 + +0.3 184,591 + -0.3 184,592 - +0.2 184,592 - -0.3 184,680 - -0.3 186,085 - +0.6 186,113 + +1.2 186,114 - -0.6 186,114 - +1.9 186,126 + -0.6 186,127 - +0.4 186,129 + +0.0 186,139 - +0.7 186,284 - +0.3 186,304 + CA265_RS00775 0.29 +2.3 186,304 + CA265_RS00775 0.29 -0.2 186,312 - CA265_RS00775 0.30 +0.8 186,324 + CA265_RS00775 0.32 -0.2 186,325 - +1.3 186,377 + CA265_RS00775 0.40 +0.6 186,378 - CA265_RS00775 0.40 +0.2 186,411 - CA265_RS00775 0.46 +0.3 186,479 - CA265_RS00775 0.56 +0.7 186,524 + CA265_RS00775 0.64 +1.0 186,524 + CA265_RS00775 0.64 +0.1 186,524 + CA265_RS00775 0.64 +0.5 186,530 + CA265_RS00775 0.65 +0.8
Or see this region's nucleotide sequence