Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25635 and BPHYT_RS25640 are separated by 199 nucleotides BPHYT_RS25640 and BPHYT_RS25645 are separated by 91 nucleotides BPHYT_RS25645 and BPHYT_RS25650 overlap by 4 nucleotides
BPHYT_RS25635: BPHYT_RS25635 - 2-dehydropantoate 2-reductase, at 1,323,788 to 1,324,684
_RS25635
BPHYT_RS25640: BPHYT_RS25640 - MFS transporter, at 1,324,884 to 1,326,218
_RS25640
BPHYT_RS25645: BPHYT_RS25645 - dehydrogenase, at 1,326,310 to 1,327,203
_RS25645
BPHYT_RS25650: BPHYT_RS25650 - dihydroxy-acid dehydratase, at 1,327,200 to 1,328,966
_RS25650
Position (kb) 1324
1325
1326
1327 Strain fitness (log2 ratio) -2
-1
0
1 at 1323.943 kb on + strand, within BPHYT_RS25635 at 1324.047 kb on - strand, within BPHYT_RS25635 at 1324.051 kb on - strand, within BPHYT_RS25635 at 1324.179 kb on - strand, within BPHYT_RS25635 at 1324.344 kb on + strand, within BPHYT_RS25635 at 1324.344 kb on + strand, within BPHYT_RS25635 at 1324.413 kb on - strand, within BPHYT_RS25635 at 1324.688 kb on + strand at 1324.688 kb on + strand at 1324.689 kb on - strand at 1324.690 kb on + strand at 1324.746 kb on + strand at 1324.826 kb on - strand at 1324.878 kb on + strand at 1324.879 kb on - strand at 1325.021 kb on + strand, within BPHYT_RS25640 at 1325.106 kb on - strand, within BPHYT_RS25640 at 1325.234 kb on - strand, within BPHYT_RS25640 at 1325.670 kb on + strand, within BPHYT_RS25640 at 1325.671 kb on - strand, within BPHYT_RS25640 at 1325.774 kb on - strand, within BPHYT_RS25640 at 1326.244 kb on + strand at 1326.245 kb on - strand at 1326.246 kb on + strand at 1326.433 kb on - strand, within BPHYT_RS25645 at 1327.003 kb on + strand, within BPHYT_RS25645
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,323,943 + BPHYT_RS25635 0.17 -0.9 1,324,047 - BPHYT_RS25635 0.29 -1.1 1,324,051 - BPHYT_RS25635 0.29 +0.0 1,324,179 - BPHYT_RS25635 0.44 -0.1 1,324,344 + BPHYT_RS25635 0.62 -0.4 1,324,344 + BPHYT_RS25635 0.62 -0.2 1,324,413 - BPHYT_RS25635 0.70 -1.2 1,324,688 + -0.5 1,324,688 + -0.6 1,324,689 - -1.0 1,324,690 + +0.5 1,324,746 + -1.1 1,324,826 - -2.6 1,324,878 + -0.9 1,324,879 - +0.1 1,325,021 + BPHYT_RS25640 0.10 -0.3 1,325,106 - BPHYT_RS25640 0.17 -0.6 1,325,234 - BPHYT_RS25640 0.26 -1.0 1,325,670 + BPHYT_RS25640 0.59 -1.7 1,325,671 - BPHYT_RS25640 0.59 -0.6 1,325,774 - BPHYT_RS25640 0.67 -0.5 1,326,244 + +0.1 1,326,245 - -0.3 1,326,246 + -0.2 1,326,433 - BPHYT_RS25645 0.14 -0.2 1,327,003 + BPHYT_RS25645 0.78 -0.0
Or see this region's nucleotide sequence