Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12990 and BPHYT_RS12995 are separated by 741 nucleotides BPHYT_RS12995 and BPHYT_RS13000 are separated by 79 nucleotides BPHYT_RS13000 and BPHYT_RS13005 are separated by 100 nucleotides
BPHYT_RS12990: BPHYT_RS12990 - iron transporter, at 2,971,006 to 2,971,272
_RS12990
BPHYT_RS12995: BPHYT_RS12995 - MFS transporter, at 2,972,014 to 2,973,483
_RS12995
BPHYT_RS13000: BPHYT_RS13000 - phosphodiesterase, at 2,973,563 to 2,974,051
_RS13000
BPHYT_RS13005: BPHYT_RS13005 - membrane protein, at 2,974,152 to 2,974,958
_RS13005
Position (kb)
2972
2973
2974 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2971.087 kb on + strand, within BPHYT_RS12990 at 2971.088 kb on - strand, within BPHYT_RS12990 at 2971.249 kb on + strand at 2971.249 kb on + strand at 2971.270 kb on + strand at 2971.270 kb on + strand at 2971.275 kb on + strand at 2971.275 kb on + strand at 2971.275 kb on + strand at 2971.276 kb on - strand at 2971.293 kb on + strand at 2973.454 kb on + strand at 2973.564 kb on + strand at 2973.862 kb on + strand, within BPHYT_RS13000 at 2973.963 kb on + strand, within BPHYT_RS13000
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,971,087 + BPHYT_RS12990 0.30 -2.6 2,971,088 - BPHYT_RS12990 0.31 -3.3 2,971,249 + -0.0 2,971,249 + +0.6 2,971,270 + -0.2 2,971,270 + -0.1 2,971,275 + +0.4 2,971,275 + -0.0 2,971,275 + -0.1 2,971,276 - -0.1 2,971,293 + +0.1 2,973,454 + -0.9 2,973,564 + +0.6 2,973,862 + BPHYT_RS13000 0.61 -0.3 2,973,963 + BPHYT_RS13000 0.82 -0.4
Or see this region's nucleotide sequence