Experiment: R2A with nitrite 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25375 and CA265_RS25380 are separated by 29 nucleotides CA265_RS25380 and CA265_RS25385 overlap by 19 nucleotides CA265_RS25385 and CA265_RS25390 are separated by 16 nucleotides CA265_RS25390 and CA265_RS25395 are separated by 31 nucleotides CA265_RS25395 and CA265_RS25400 are separated by 81 nucleotides
CA265_RS25375: CA265_RS25375 - hypothetical protein, at 6,166,125 to 6,166,799
_RS25375
CA265_RS25380: CA265_RS25380 - hypothetical protein, at 6,166,829 to 6,167,038
_RS25380
CA265_RS25385: CA265_RS25385 - Txe/YoeB family addiction module toxin, at 6,167,020 to 6,167,301
_RS25385
CA265_RS25390: CA265_RS25390 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 6,167,318 to 6,168,169
_RS25390
CA265_RS25395: CA265_RS25395 - cysteine hydrolase, at 6,168,201 to 6,168,743
_RS25395
CA265_RS25400: CA265_RS25400 - fatty acid desaturase, at 6,168,825 to 6,169,451
_RS25400
Position (kb)
6167
6168
6169 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6166.324 kb on + strand, within CA265_RS25375 at 6166.342 kb on + strand, within CA265_RS25375 at 6166.400 kb on + strand, within CA265_RS25375 at 6166.408 kb on - strand, within CA265_RS25375 at 6166.411 kb on - strand, within CA265_RS25375 at 6166.454 kb on - strand, within CA265_RS25375 at 6166.553 kb on + strand, within CA265_RS25375 at 6166.562 kb on - strand, within CA265_RS25375 at 6166.687 kb on - strand, within CA265_RS25375 at 6166.716 kb on + strand, within CA265_RS25375 at 6166.717 kb on - strand, within CA265_RS25375 at 6166.748 kb on - strand at 6166.748 kb on - strand at 6166.766 kb on - strand at 6167.036 kb on + strand at 6167.055 kb on + strand, within CA265_RS25385 at 6167.091 kb on + strand, within CA265_RS25385 at 6167.132 kb on + strand, within CA265_RS25385 at 6167.132 kb on + strand, within CA265_RS25385 at 6167.231 kb on + strand, within CA265_RS25385 at 6167.231 kb on + strand, within CA265_RS25385 at 6167.233 kb on + strand, within CA265_RS25385 at 6167.256 kb on + strand, within CA265_RS25385 at 6168.186 kb on - strand at 6168.188 kb on - strand at 6168.191 kb on + strand at 6168.220 kb on + strand at 6168.290 kb on - strand, within CA265_RS25395 at 6168.294 kb on - strand, within CA265_RS25395 at 6168.450 kb on - strand, within CA265_RS25395 at 6168.484 kb on + strand, within CA265_RS25395 at 6168.484 kb on + strand, within CA265_RS25395 at 6168.579 kb on + strand, within CA265_RS25395 at 6168.631 kb on + strand, within CA265_RS25395 at 6168.646 kb on + strand, within CA265_RS25395 at 6168.710 kb on - strand at 6168.717 kb on - strand at 6168.742 kb on - strand at 6168.786 kb on - strand at 6168.786 kb on - strand at 6168.787 kb on + strand at 6168.795 kb on + strand at 6168.795 kb on + strand at 6168.796 kb on - strand at 6168.818 kb on - strand at 6168.824 kb on - strand at 6168.861 kb on - strand at 6168.861 kb on - strand at 6168.894 kb on + strand, within CA265_RS25400 at 6169.081 kb on + strand, within CA265_RS25400 at 6169.132 kb on - strand, within CA265_RS25400 at 6169.153 kb on - strand, within CA265_RS25400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 1.5 mM remove 6,166,324 + CA265_RS25375 0.29 -0.0 6,166,342 + CA265_RS25375 0.32 +0.1 6,166,400 + CA265_RS25375 0.41 +0.3 6,166,408 - CA265_RS25375 0.42 +0.5 6,166,411 - CA265_RS25375 0.42 +0.1 6,166,454 - CA265_RS25375 0.49 +1.8 6,166,553 + CA265_RS25375 0.63 -1.9 6,166,562 - CA265_RS25375 0.65 +0.3 6,166,687 - CA265_RS25375 0.83 -1.0 6,166,716 + CA265_RS25375 0.88 +0.5 6,166,717 - CA265_RS25375 0.88 +0.3 6,166,748 - +0.1 6,166,748 - -1.1 6,166,766 - +0.2 6,167,036 + -0.8 6,167,055 + CA265_RS25385 0.12 -0.2 6,167,091 + CA265_RS25385 0.25 +0.2 6,167,132 + CA265_RS25385 0.40 -0.3 6,167,132 + CA265_RS25385 0.40 +0.3 6,167,231 + CA265_RS25385 0.75 -0.2 6,167,231 + CA265_RS25385 0.75 +0.1 6,167,233 + CA265_RS25385 0.76 +0.8 6,167,256 + CA265_RS25385 0.84 -0.3 6,168,186 - +0.1 6,168,188 - +0.7 6,168,191 + -0.0 6,168,220 + +0.0 6,168,290 - CA265_RS25395 0.16 +0.7 6,168,294 - CA265_RS25395 0.17 +0.5 6,168,450 - CA265_RS25395 0.46 +0.9 6,168,484 + CA265_RS25395 0.52 +1.3 6,168,484 + CA265_RS25395 0.52 +0.6 6,168,579 + CA265_RS25395 0.70 +1.2 6,168,631 + CA265_RS25395 0.79 +1.0 6,168,646 + CA265_RS25395 0.82 -0.0 6,168,710 - -0.2 6,168,717 - +1.9 6,168,742 - +0.1 6,168,786 - +0.6 6,168,786 - +0.2 6,168,787 + +1.6 6,168,795 + -0.6 6,168,795 + -1.5 6,168,796 - +0.4 6,168,818 - -0.8 6,168,824 - +0.8 6,168,861 - -0.2 6,168,861 - -0.3 6,168,894 + CA265_RS25400 0.11 -1.4 6,169,081 + CA265_RS25400 0.41 -0.2 6,169,132 - CA265_RS25400 0.49 +0.2 6,169,153 - CA265_RS25400 0.52 +0.2
Or see this region's nucleotide sequence