Experiment: R2A with nitrite 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11690 and CA265_RS11695 are separated by 92 nucleotides CA265_RS11695 and CA265_RS11700 are separated by 180 nucleotides CA265_RS11700 and CA265_RS11705 are separated by 303 nucleotides CA265_RS11705 and CA265_RS11710 are separated by 68 nucleotides
CA265_RS11690: CA265_RS11690 - DNA-binding protein, at 2,898,674 to 2,899,216
_RS11690
CA265_RS11695: CA265_RS11695 - hypothetical protein, at 2,899,309 to 2,899,875
_RS11695
CA265_RS11700: CA265_RS11700 - hypothetical protein, at 2,900,056 to 2,900,451
_RS11700
CA265_RS11705: CA265_RS11705 - hypothetical protein, at 2,900,755 to 2,901,321
_RS11705
CA265_RS11710: CA265_RS11710 - hypothetical protein, at 2,901,390 to 2,902,523
_RS11710
Position (kb)
2900
2901 Strain fitness (log2 ratio)
-2
-1
0
1 at 2899.059 kb on + strand, within CA265_RS11690 at 2899.140 kb on + strand, within CA265_RS11690 at 2899.212 kb on + strand at 2899.213 kb on - strand at 2899.213 kb on - strand at 2899.213 kb on - strand at 2899.214 kb on + strand at 2899.215 kb on - strand at 2899.215 kb on - strand at 2899.215 kb on - strand at 2899.256 kb on - strand at 2899.390 kb on + strand, within CA265_RS11695 at 2899.444 kb on - strand, within CA265_RS11695 at 2899.498 kb on + strand, within CA265_RS11695 at 2899.600 kb on - strand, within CA265_RS11695 at 2899.633 kb on + strand, within CA265_RS11695 at 2899.726 kb on + strand, within CA265_RS11695 at 2899.742 kb on + strand, within CA265_RS11695 at 2899.742 kb on + strand, within CA265_RS11695 at 2899.746 kb on + strand, within CA265_RS11695 at 2899.747 kb on - strand, within CA265_RS11695 at 2899.747 kb on - strand, within CA265_RS11695 at 2899.784 kb on + strand, within CA265_RS11695 at 2899.784 kb on + strand, within CA265_RS11695 at 2899.784 kb on + strand, within CA265_RS11695 at 2899.785 kb on - strand, within CA265_RS11695 at 2899.792 kb on - strand, within CA265_RS11695 at 2899.795 kb on - strand, within CA265_RS11695 at 2899.795 kb on - strand, within CA265_RS11695 at 2899.977 kb on + strand at 2900.019 kb on + strand at 2900.029 kb on - strand at 2900.100 kb on + strand, within CA265_RS11700 at 2900.127 kb on + strand, within CA265_RS11700 at 2900.127 kb on + strand, within CA265_RS11700 at 2900.249 kb on + strand, within CA265_RS11700 at 2900.249 kb on + strand, within CA265_RS11700 at 2900.250 kb on - strand, within CA265_RS11700 at 2900.338 kb on + strand, within CA265_RS11700 at 2900.341 kb on + strand, within CA265_RS11700 at 2900.341 kb on + strand, within CA265_RS11700 at 2900.351 kb on + strand, within CA265_RS11700 at 2900.352 kb on - strand, within CA265_RS11700 at 2900.352 kb on - strand, within CA265_RS11700 at 2900.414 kb on + strand at 2900.414 kb on + strand at 2900.449 kb on + strand at 2900.492 kb on + strand at 2900.493 kb on - strand at 2900.550 kb on + strand at 2900.575 kb on - strand at 2900.591 kb on - strand at 2900.632 kb on - strand at 2900.772 kb on + strand at 2900.775 kb on - strand at 2900.793 kb on - strand at 2900.837 kb on - strand, within CA265_RS11705 at 2900.843 kb on - strand, within CA265_RS11705 at 2900.846 kb on - strand, within CA265_RS11705 at 2900.858 kb on + strand, within CA265_RS11705 at 2900.933 kb on + strand, within CA265_RS11705 at 2900.934 kb on - strand, within CA265_RS11705 at 2900.984 kb on + strand, within CA265_RS11705 at 2900.987 kb on - strand, within CA265_RS11705 at 2901.051 kb on + strand, within CA265_RS11705 at 2901.054 kb on + strand, within CA265_RS11705 at 2901.102 kb on - strand, within CA265_RS11705 at 2901.105 kb on - strand, within CA265_RS11705 at 2901.152 kb on + strand, within CA265_RS11705 at 2901.206 kb on - strand, within CA265_RS11705 at 2901.206 kb on - strand, within CA265_RS11705 at 2901.227 kb on + strand, within CA265_RS11705 at 2901.232 kb on - strand, within CA265_RS11705 at 2901.323 kb on + strand at 2901.339 kb on + strand at 2901.367 kb on - strand at 2901.371 kb on - strand at 2901.372 kb on + strand at 2901.373 kb on - strand at 2901.373 kb on - strand at 2901.374 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 1.5 mM remove 2,899,059 + CA265_RS11690 0.71 -0.8 2,899,140 + CA265_RS11690 0.86 -0.3 2,899,212 + +1.3 2,899,213 - -0.3 2,899,213 - +0.2 2,899,213 - -0.8 2,899,214 + -0.0 2,899,215 - -0.3 2,899,215 - -0.9 2,899,215 - -0.3 2,899,256 - +0.3 2,899,390 + CA265_RS11695 0.14 +0.2 2,899,444 - CA265_RS11695 0.24 -1.5 2,899,498 + CA265_RS11695 0.33 -0.5 2,899,600 - CA265_RS11695 0.51 +0.6 2,899,633 + CA265_RS11695 0.57 +0.0 2,899,726 + CA265_RS11695 0.74 +0.5 2,899,742 + CA265_RS11695 0.76 -0.7 2,899,742 + CA265_RS11695 0.76 -0.1 2,899,746 + CA265_RS11695 0.77 -1.4 2,899,747 - CA265_RS11695 0.77 -0.2 2,899,747 - CA265_RS11695 0.77 -0.2 2,899,784 + CA265_RS11695 0.84 -0.4 2,899,784 + CA265_RS11695 0.84 -0.5 2,899,784 + CA265_RS11695 0.84 -0.1 2,899,785 - CA265_RS11695 0.84 -2.0 2,899,792 - CA265_RS11695 0.85 -0.1 2,899,795 - CA265_RS11695 0.86 -2.0 2,899,795 - CA265_RS11695 0.86 -0.0 2,899,977 + +0.6 2,900,019 + +0.5 2,900,029 - -0.5 2,900,100 + CA265_RS11700 0.11 +0.6 2,900,127 + CA265_RS11700 0.18 -1.5 2,900,127 + CA265_RS11700 0.18 -0.1 2,900,249 + CA265_RS11700 0.49 +0.7 2,900,249 + CA265_RS11700 0.49 +0.0 2,900,250 - CA265_RS11700 0.49 +0.5 2,900,338 + CA265_RS11700 0.71 +0.5 2,900,341 + CA265_RS11700 0.72 +0.2 2,900,341 + CA265_RS11700 0.72 -0.3 2,900,351 + CA265_RS11700 0.74 +0.4 2,900,352 - CA265_RS11700 0.75 +0.6 2,900,352 - CA265_RS11700 0.75 +0.0 2,900,414 + +0.3 2,900,414 + +0.2 2,900,449 + -0.0 2,900,492 + -0.2 2,900,493 - +0.0 2,900,550 + +0.3 2,900,575 - -0.0 2,900,591 - +0.0 2,900,632 - +1.5 2,900,772 + -0.2 2,900,775 - -0.1 2,900,793 - +0.0 2,900,837 - CA265_RS11705 0.14 -0.1 2,900,843 - CA265_RS11705 0.16 +0.5 2,900,846 - CA265_RS11705 0.16 +0.3 2,900,858 + CA265_RS11705 0.18 -0.2 2,900,933 + CA265_RS11705 0.31 -0.6 2,900,934 - CA265_RS11705 0.32 +0.4 2,900,984 + CA265_RS11705 0.40 -0.1 2,900,987 - CA265_RS11705 0.41 -0.2 2,901,051 + CA265_RS11705 0.52 -0.2 2,901,054 + CA265_RS11705 0.53 +0.1 2,901,102 - CA265_RS11705 0.61 +0.3 2,901,105 - CA265_RS11705 0.62 +0.1 2,901,152 + CA265_RS11705 0.70 -0.3 2,901,206 - CA265_RS11705 0.80 -0.2 2,901,206 - CA265_RS11705 0.80 -0.3 2,901,227 + CA265_RS11705 0.83 +0.3 2,901,232 - CA265_RS11705 0.84 +0.2 2,901,323 + +0.3 2,901,339 + +0.1 2,901,367 - -0.0 2,901,371 - -0.6 2,901,372 + -0.4 2,901,373 - +0.3 2,901,373 - -0.2 2,901,374 + -1.1
Or see this region's nucleotide sequence