Experiment: KB with Chloramphenicol 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1011 and Psyr_1012 overlap by 32 nucleotides Psyr_1012 and Psyr_1013 are separated by 180 nucleotides Psyr_1013 and Psyr_1014 are separated by 316 nucleotides
Psyr_1011: Psyr_1011 - NADH:flavin oxidoreductase/NADH oxidase, at 1,145,082 to 1,146,185
_1011
Psyr_1012: Psyr_1012 - Glycosyl transferase, group 1, at 1,146,154 to 1,147,374
_1012
Psyr_1013: Psyr_1013 - hypothetical protein, at 1,147,555 to 1,147,875
_1013
Psyr_1014: Psyr_1014 - conserved domain protein, at 1,148,192 to 1,148,536
_1014
Position (kb) 1146
1147
1148 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1145.258 kb on + strand, within Psyr_1011 at 1145.259 kb on - strand, within Psyr_1011 at 1145.643 kb on - strand, within Psyr_1011 at 1145.772 kb on + strand, within Psyr_1011 at 1145.772 kb on + strand, within Psyr_1011 at 1145.773 kb on - strand, within Psyr_1011 at 1145.847 kb on - strand, within Psyr_1011 at 1145.948 kb on + strand, within Psyr_1011 at 1145.948 kb on + strand, within Psyr_1011 at 1145.949 kb on - strand, within Psyr_1011 at 1145.949 kb on - strand, within Psyr_1011 at 1146.429 kb on + strand, within Psyr_1012 at 1146.456 kb on - strand, within Psyr_1012 at 1146.538 kb on - strand, within Psyr_1012 at 1146.551 kb on + strand, within Psyr_1012 at 1146.551 kb on + strand, within Psyr_1012 at 1146.663 kb on + strand, within Psyr_1012 at 1146.664 kb on - strand, within Psyr_1012 at 1146.944 kb on + strand, within Psyr_1012 at 1146.945 kb on - strand, within Psyr_1012 at 1146.945 kb on - strand, within Psyr_1012 at 1146.980 kb on + strand, within Psyr_1012 at 1146.980 kb on + strand, within Psyr_1012 at 1146.990 kb on + strand, within Psyr_1012 at 1146.990 kb on + strand, within Psyr_1012 at 1146.990 kb on + strand, within Psyr_1012 at 1146.990 kb on + strand, within Psyr_1012 at 1146.991 kb on - strand, within Psyr_1012 at 1146.991 kb on - strand, within Psyr_1012 at 1146.991 kb on - strand, within Psyr_1012 at 1146.991 kb on - strand, within Psyr_1012 at 1146.991 kb on - strand, within Psyr_1012 at 1146.993 kb on - strand, within Psyr_1012 at 1146.993 kb on - strand, within Psyr_1012 at 1146.993 kb on - strand, within Psyr_1012 at 1147.091 kb on + strand, within Psyr_1012 at 1147.091 kb on + strand, within Psyr_1012 at 1147.091 kb on + strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.092 kb on - strand, within Psyr_1012 at 1147.333 kb on - strand at 1147.359 kb on + strand at 1147.394 kb on + strand at 1147.394 kb on + strand at 1147.395 kb on - strand at 1147.405 kb on + strand at 1147.416 kb on + strand at 1147.416 kb on + strand at 1147.435 kb on - strand at 1147.479 kb on + strand at 1147.483 kb on + strand at 1147.483 kb on + strand at 1147.484 kb on - strand at 1147.484 kb on - strand at 1147.484 kb on - strand at 1147.484 kb on - strand at 1147.484 kb on - strand at 1147.484 kb on - strand at 1147.486 kb on + strand at 1147.486 kb on + strand at 1147.486 kb on + strand at 1147.486 kb on + strand at 1147.487 kb on - strand at 1147.498 kb on + strand at 1147.498 kb on + strand at 1147.498 kb on + strand at 1147.498 kb on + strand at 1147.498 kb on + strand at 1147.499 kb on - strand at 1147.499 kb on - strand at 1147.540 kb on + strand at 1147.540 kb on + strand at 1147.541 kb on - strand at 1147.541 kb on - strand at 1147.541 kb on - strand at 1147.596 kb on + strand, within Psyr_1013 at 1147.597 kb on - strand, within Psyr_1013 at 1147.597 kb on - strand, within Psyr_1013 at 1147.651 kb on - strand, within Psyr_1013 at 1147.808 kb on + strand, within Psyr_1013 at 1147.858 kb on + strand at 1147.858 kb on + strand at 1147.859 kb on - strand at 1147.900 kb on - strand at 1147.900 kb on - strand at 1147.903 kb on + strand at 1147.903 kb on + strand at 1147.904 kb on - strand at 1147.907 kb on - strand at 1147.921 kb on + strand at 1147.940 kb on - strand at 1147.957 kb on + strand at 1147.957 kb on + strand at 1147.957 kb on + strand at 1147.957 kb on + strand at 1147.957 kb on + strand at 1147.958 kb on - strand at 1147.984 kb on - strand at 1148.054 kb on - strand at 1148.055 kb on + strand at 1148.055 kb on + strand at 1148.055 kb on + strand at 1148.055 kb on + strand at 1148.056 kb on - strand at 1148.056 kb on - strand at 1148.056 kb on - strand at 1148.057 kb on + strand at 1148.057 kb on + strand at 1148.057 kb on + strand at 1148.057 kb on + strand at 1148.058 kb on - strand at 1148.058 kb on - strand at 1148.058 kb on - strand at 1148.080 kb on + strand at 1148.080 kb on + strand at 1148.081 kb on - strand at 1148.081 kb on - strand at 1148.081 kb on - strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.082 kb on + strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.083 kb on - strand at 1148.084 kb on + strand at 1148.084 kb on + strand at 1148.084 kb on + strand at 1148.084 kb on + strand at 1148.084 kb on + strand at 1148.085 kb on - strand at 1148.091 kb on - strand at 1148.128 kb on + strand at 1148.129 kb on - strand at 1148.161 kb on + strand at 1148.274 kb on + strand, within Psyr_1014 at 1148.275 kb on - strand, within Psyr_1014 at 1148.280 kb on + strand, within Psyr_1014 at 1148.281 kb on - strand, within Psyr_1014 at 1148.316 kb on + strand, within Psyr_1014 at 1148.316 kb on + strand, within Psyr_1014
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Chloramphenicol 12.5 ug/ml remove 1,145,258 + Psyr_1011 0.16 +0.9 1,145,259 - Psyr_1011 0.16 +0.0 1,145,643 - Psyr_1011 0.51 -0.5 1,145,772 + Psyr_1011 0.62 +0.1 1,145,772 + Psyr_1011 0.62 +0.0 1,145,773 - Psyr_1011 0.63 -0.4 1,145,847 - Psyr_1011 0.69 +0.0 1,145,948 + Psyr_1011 0.78 +0.2 1,145,948 + Psyr_1011 0.78 -0.0 1,145,949 - Psyr_1011 0.79 +0.9 1,145,949 - Psyr_1011 0.79 -0.4 1,146,429 + Psyr_1012 0.23 -0.7 1,146,456 - Psyr_1012 0.25 +0.9 1,146,538 - Psyr_1012 0.31 -0.0 1,146,551 + Psyr_1012 0.33 +0.2 1,146,551 + Psyr_1012 0.33 +0.7 1,146,663 + Psyr_1012 0.42 +0.6 1,146,664 - Psyr_1012 0.42 +1.7 1,146,944 + Psyr_1012 0.65 +0.5 1,146,945 - Psyr_1012 0.65 +0.2 1,146,945 - Psyr_1012 0.65 +0.3 1,146,980 + Psyr_1012 0.68 +0.6 1,146,980 + Psyr_1012 0.68 -0.1 1,146,990 + Psyr_1012 0.68 +3.1 1,146,990 + Psyr_1012 0.68 +0.5 1,146,990 + Psyr_1012 0.68 -3.6 1,146,990 + Psyr_1012 0.68 +0.3 1,146,991 - Psyr_1012 0.69 +0.9 1,146,991 - Psyr_1012 0.69 +0.4 1,146,991 - Psyr_1012 0.69 +0.4 1,146,991 - Psyr_1012 0.69 -0.2 1,146,991 - Psyr_1012 0.69 +0.9 1,146,993 - Psyr_1012 0.69 +0.5 1,146,993 - Psyr_1012 0.69 +0.6 1,146,993 - Psyr_1012 0.69 -2.4 1,147,091 + Psyr_1012 0.77 -1.0 1,147,091 + Psyr_1012 0.77 -0.3 1,147,091 + Psyr_1012 0.77 +0.2 1,147,092 - Psyr_1012 0.77 +0.1 1,147,092 - Psyr_1012 0.77 -0.7 1,147,092 - Psyr_1012 0.77 -1.0 1,147,092 - Psyr_1012 0.77 +1.2 1,147,092 - Psyr_1012 0.77 -1.1 1,147,092 - Psyr_1012 0.77 +0.4 1,147,092 - Psyr_1012 0.77 +1.4 1,147,092 - Psyr_1012 0.77 -1.8 1,147,333 - +0.2 1,147,359 + +0.2 1,147,394 + +0.7 1,147,394 + -0.3 1,147,395 - -1.2 1,147,405 + -0.0 1,147,416 + +0.5 1,147,416 + -0.1 1,147,435 - -0.3 1,147,479 + -0.4 1,147,483 + +0.6 1,147,483 + -1.3 1,147,484 - -0.6 1,147,484 - -1.0 1,147,484 - +0.2 1,147,484 - -1.4 1,147,484 - -1.3 1,147,484 - -1.9 1,147,486 + -0.7 1,147,486 + -0.1 1,147,486 + -0.3 1,147,486 + +0.6 1,147,487 - -0.5 1,147,498 + +0.0 1,147,498 + -0.4 1,147,498 + -1.1 1,147,498 + +0.4 1,147,498 + +0.4 1,147,499 - -0.1 1,147,499 - -0.5 1,147,540 + +0.4 1,147,540 + -1.2 1,147,541 - -0.5 1,147,541 - +1.1 1,147,541 - +0.0 1,147,596 + Psyr_1013 0.13 +1.5 1,147,597 - Psyr_1013 0.13 -0.4 1,147,597 - Psyr_1013 0.13 -0.6 1,147,651 - Psyr_1013 0.30 +0.2 1,147,808 + Psyr_1013 0.79 +0.6 1,147,858 + +0.5 1,147,858 + +0.9 1,147,859 - +1.5 1,147,900 - -0.1 1,147,900 - +1.1 1,147,903 + +0.2 1,147,903 + +0.1 1,147,904 - -1.0 1,147,907 - -1.9 1,147,921 + -0.4 1,147,940 - +1.7 1,147,957 + +0.5 1,147,957 + +0.9 1,147,957 + -0.4 1,147,957 + -0.1 1,147,957 + -1.7 1,147,958 - +1.2 1,147,984 - +2.2 1,148,054 - -0.2 1,148,055 + -0.1 1,148,055 + -0.3 1,148,055 + -0.8 1,148,055 + -1.4 1,148,056 - +2.3 1,148,056 - +0.4 1,148,056 - +0.5 1,148,057 + +0.5 1,148,057 + -0.1 1,148,057 + +0.9 1,148,057 + -1.4 1,148,058 - -1.1 1,148,058 - -1.2 1,148,058 - -0.3 1,148,080 + -0.2 1,148,080 + +0.6 1,148,081 - -0.6 1,148,081 - -0.6 1,148,081 - -0.3 1,148,082 + -0.0 1,148,082 + +1.6 1,148,082 + +1.0 1,148,082 + +1.1 1,148,082 + -0.1 1,148,082 + -0.7 1,148,082 + +0.2 1,148,082 + -0.8 1,148,083 - -0.1 1,148,083 - +1.1 1,148,083 - -0.7 1,148,083 - -0.4 1,148,083 - -1.4 1,148,083 - -0.6 1,148,083 - +2.2 1,148,083 - -0.1 1,148,084 + +0.0 1,148,084 + +0.7 1,148,084 + -0.3 1,148,084 + -1.1 1,148,084 + -0.6 1,148,085 - -1.4 1,148,091 - -0.2 1,148,128 + +1.3 1,148,129 - +1.1 1,148,161 + +0.3 1,148,274 + Psyr_1014 0.24 +0.3 1,148,275 - Psyr_1014 0.24 -0.6 1,148,280 + Psyr_1014 0.26 -1.8 1,148,281 - Psyr_1014 0.26 +1.2 1,148,316 + Psyr_1014 0.36 +0.4 1,148,316 + Psyr_1014 0.36 -0.0
Or see this region's nucleotide sequence