Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5436 and Pf6N2E2_5437 are separated by 145 nucleotides Pf6N2E2_5437 and Pf6N2E2_5438 are separated by 16 nucleotides Pf6N2E2_5438 and Pf6N2E2_5439 overlap by 4 nucleotides Pf6N2E2_5439 and Pf6N2E2_5440 are separated by 317 nucleotides
Pf6N2E2_5436 - MutT/nudix family protein, at 6,243,638 to 6,244,189
_5436
Pf6N2E2_5437 - Hypothetical nudix hydrolase YeaB, at 6,244,335 to 6,244,934
_5437
Pf6N2E2_5438 - carbonic anhydrase, family 3, at 6,244,951 to 6,245,475
_5438
Pf6N2E2_5439 - FIG00954164: hypothetical protein, at 6,245,472 to 6,245,681
_5439
Pf6N2E2_5440 - Mobile element protein, at 6,245,999 to 6,247,267
_5440
Position (kb)
6244
6245
6246 Strain fitness (log2 ratio)
-2
-1
0
1 at 6243.999 kb on + strand, within Pf6N2E2_5436 at 6244.043 kb on - strand, within Pf6N2E2_5436 at 6244.319 kb on + strand at 6244.348 kb on + strand at 6244.348 kb on + strand at 6244.360 kb on + strand at 6244.361 kb on - strand at 6244.361 kb on - strand at 6244.361 kb on - strand at 6244.364 kb on + strand at 6244.364 kb on + strand at 6244.364 kb on + strand at 6244.364 kb on + strand at 6244.364 kb on + strand at 6244.365 kb on - strand at 6244.370 kb on + strand at 6244.370 kb on + strand at 6244.659 kb on + strand, within Pf6N2E2_5437 at 6244.659 kb on + strand, within Pf6N2E2_5437 at 6244.659 kb on + strand, within Pf6N2E2_5437 at 6244.659 kb on + strand, within Pf6N2E2_5437 at 6244.660 kb on - strand, within Pf6N2E2_5437 at 6244.660 kb on - strand, within Pf6N2E2_5437 at 6244.660 kb on - strand, within Pf6N2E2_5437 at 6244.660 kb on - strand, within Pf6N2E2_5437 at 6244.680 kb on + strand, within Pf6N2E2_5437 at 6244.680 kb on + strand, within Pf6N2E2_5437 at 6244.681 kb on - strand, within Pf6N2E2_5437 at 6244.689 kb on + strand, within Pf6N2E2_5437 at 6244.690 kb on - strand, within Pf6N2E2_5437 at 6244.782 kb on + strand, within Pf6N2E2_5437 at 6244.800 kb on + strand, within Pf6N2E2_5437 at 6244.800 kb on + strand, within Pf6N2E2_5437 at 6244.800 kb on + strand, within Pf6N2E2_5437 at 6244.800 kb on + strand, within Pf6N2E2_5437 at 6244.800 kb on + strand, within Pf6N2E2_5437 at 6244.800 kb on + strand, within Pf6N2E2_5437 at 6244.801 kb on - strand, within Pf6N2E2_5437 at 6244.801 kb on - strand, within Pf6N2E2_5437 at 6244.827 kb on + strand, within Pf6N2E2_5437 at 6244.827 kb on + strand, within Pf6N2E2_5437 at 6244.828 kb on - strand, within Pf6N2E2_5437 at 6244.828 kb on - strand, within Pf6N2E2_5437 at 6244.881 kb on + strand at 6244.882 kb on - strand at 6244.928 kb on + strand at 6244.928 kb on + strand at 6244.929 kb on - strand at 6244.929 kb on - strand at 6244.957 kb on + strand at 6244.958 kb on - strand at 6245.120 kb on + strand, within Pf6N2E2_5438 at 6245.120 kb on + strand, within Pf6N2E2_5438 at 6245.120 kb on + strand, within Pf6N2E2_5438 at 6245.120 kb on + strand, within Pf6N2E2_5438 at 6245.121 kb on - strand, within Pf6N2E2_5438 at 6245.227 kb on + strand, within Pf6N2E2_5438 at 6245.227 kb on + strand, within Pf6N2E2_5438 at 6245.227 kb on + strand, within Pf6N2E2_5438 at 6245.228 kb on - strand, within Pf6N2E2_5438 at 6245.422 kb on + strand, within Pf6N2E2_5438 at 6245.422 kb on + strand, within Pf6N2E2_5438 at 6245.422 kb on + strand, within Pf6N2E2_5438 at 6245.422 kb on + strand, within Pf6N2E2_5438 at 6245.422 kb on + strand, within Pf6N2E2_5438 at 6245.423 kb on - strand at 6245.423 kb on - strand at 6245.443 kb on + strand at 6245.444 kb on - strand at 6245.743 kb on - strand at 6245.743 kb on - strand at 6245.743 kb on - strand at 6245.743 kb on - strand at 6245.743 kb on - strand at 6245.743 kb on - strand at 6245.743 kb on - strand at 6245.743 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 6,243,999 + Pf6N2E2_5436 0.65 +1.3 6,244,043 - Pf6N2E2_5436 0.73 -0.5 6,244,319 + -1.8 6,244,348 + -0.2 6,244,348 + +1.7 6,244,360 + -0.6 6,244,361 - -0.3 6,244,361 - +1.1 6,244,361 - -0.3 6,244,364 + +1.1 6,244,364 + +1.2 6,244,364 + -0.6 6,244,364 + +0.2 6,244,364 + -1.7 6,244,365 - -0.1 6,244,370 + +0.9 6,244,370 + -0.0 6,244,659 + Pf6N2E2_5437 0.54 +0.2 6,244,659 + Pf6N2E2_5437 0.54 -0.3 6,244,659 + Pf6N2E2_5437 0.54 +0.9 6,244,659 + Pf6N2E2_5437 0.54 -0.4 6,244,660 - Pf6N2E2_5437 0.54 -0.2 6,244,660 - Pf6N2E2_5437 0.54 -0.1 6,244,660 - Pf6N2E2_5437 0.54 +1.2 6,244,660 - Pf6N2E2_5437 0.54 -0.5 6,244,680 + Pf6N2E2_5437 0.57 -1.1 6,244,680 + Pf6N2E2_5437 0.57 -1.3 6,244,681 - Pf6N2E2_5437 0.58 -0.6 6,244,689 + Pf6N2E2_5437 0.59 +1.1 6,244,690 - Pf6N2E2_5437 0.59 +1.3 6,244,782 + Pf6N2E2_5437 0.74 +1.3 6,244,800 + Pf6N2E2_5437 0.78 -0.2 6,244,800 + Pf6N2E2_5437 0.78 -0.4 6,244,800 + Pf6N2E2_5437 0.78 -1.4 6,244,800 + Pf6N2E2_5437 0.78 -1.0 6,244,800 + Pf6N2E2_5437 0.78 +0.3 6,244,800 + Pf6N2E2_5437 0.78 -1.6 6,244,801 - Pf6N2E2_5437 0.78 -0.6 6,244,801 - Pf6N2E2_5437 0.78 +0.6 6,244,827 + Pf6N2E2_5437 0.82 -0.2 6,244,827 + Pf6N2E2_5437 0.82 +0.3 6,244,828 - Pf6N2E2_5437 0.82 +0.3 6,244,828 - Pf6N2E2_5437 0.82 -0.4 6,244,881 + -0.1 6,244,882 - +0.7 6,244,928 + -0.0 6,244,928 + +0.4 6,244,929 - -1.6 6,244,929 - +0.3 6,244,957 + +0.8 6,244,958 - -0.3 6,245,120 + Pf6N2E2_5438 0.32 +0.4 6,245,120 + Pf6N2E2_5438 0.32 +0.6 6,245,120 + Pf6N2E2_5438 0.32 -0.2 6,245,120 + Pf6N2E2_5438 0.32 +0.3 6,245,121 - Pf6N2E2_5438 0.32 +1.3 6,245,227 + Pf6N2E2_5438 0.53 +1.3 6,245,227 + Pf6N2E2_5438 0.53 +0.6 6,245,227 + Pf6N2E2_5438 0.53 +0.8 6,245,228 - Pf6N2E2_5438 0.53 -1.0 6,245,422 + Pf6N2E2_5438 0.90 +1.2 6,245,422 + Pf6N2E2_5438 0.90 +0.6 6,245,422 + Pf6N2E2_5438 0.90 +0.8 6,245,422 + Pf6N2E2_5438 0.90 +0.9 6,245,422 + Pf6N2E2_5438 0.90 -0.3 6,245,423 - +0.9 6,245,423 - +0.3 6,245,443 + -0.9 6,245,444 - +0.7 6,245,743 - +0.3 6,245,743 - -0.3 6,245,743 - +0.8 6,245,743 - -0.8 6,245,743 - -1.4 6,245,743 - +0.9 6,245,743 - -0.6 6,245,743 - -0.5
Or see this region's nucleotide sequence