Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2054 and Pf6N2E2_2055 are separated by 78 nucleotides Pf6N2E2_2055 and Pf6N2E2_2056 overlap by 11 nucleotides Pf6N2E2_2056 and Pf6N2E2_2057 are separated by 66 nucleotides Pf6N2E2_2057 and Pf6N2E2_2058 are separated by 53 nucleotides Pf6N2E2_2058 and Pf6N2E2_2059 are separated by 53 nucleotides
Pf6N2E2_2054 - hypothetical protein, at 2,444,933 to 2,445,274
_2054
Pf6N2E2_2055 - StbE replicon stabilization toxin, at 2,445,353 to 2,445,634
_2055
Pf6N2E2_2056 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 2,445,624 to 2,445,872
_2056
Pf6N2E2_2057 - Phosphatidylserine/phosphatidylglycerophosphate/ cardiolipi n synthases and related enzymes, at 2,445,939 to 2,447,585
_2057
Pf6N2E2_2058 - ChlD component of cobalt chelatase involved in B12 biosynthesis, at 2,447,639 to 2,448,250
_2058
Pf6N2E2_2059 - ChlI component of cobalt chelatase involved in B12 biosynthesis, at 2,448,304 to 2,449,308
_2059
Position (kb)
2445
2446
2447
2448 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2444.946 kb on + strand at 2444.947 kb on - strand at 2444.947 kb on - strand at 2444.947 kb on - strand at 2444.976 kb on + strand, within Pf6N2E2_2054 at 2444.976 kb on + strand, within Pf6N2E2_2054 at 2444.976 kb on + strand, within Pf6N2E2_2054 at 2444.977 kb on - strand, within Pf6N2E2_2054 at 2444.977 kb on - strand, within Pf6N2E2_2054 at 2444.977 kb on - strand, within Pf6N2E2_2054 at 2445.004 kb on - strand, within Pf6N2E2_2054 at 2445.004 kb on - strand, within Pf6N2E2_2054 at 2445.245 kb on + strand at 2445.245 kb on + strand at 2445.246 kb on - strand at 2445.246 kb on - strand at 2445.303 kb on - strand at 2445.303 kb on - strand at 2445.308 kb on - strand at 2445.376 kb on - strand at 2445.434 kb on + strand, within Pf6N2E2_2055 at 2445.435 kb on - strand, within Pf6N2E2_2055 at 2445.435 kb on - strand, within Pf6N2E2_2055 at 2445.435 kb on - strand, within Pf6N2E2_2055 at 2445.435 kb on - strand, within Pf6N2E2_2055 at 2445.482 kb on - strand, within Pf6N2E2_2055 at 2445.627 kb on + strand at 2445.627 kb on + strand at 2445.627 kb on + strand at 2445.628 kb on - strand at 2445.628 kb on - strand at 2445.991 kb on + strand at 2445.994 kb on + strand at 2445.994 kb on + strand at 2445.995 kb on - strand at 2445.995 kb on - strand at 2445.995 kb on - strand at 2445.995 kb on - strand at 2445.995 kb on - strand at 2445.995 kb on - strand at 2446.064 kb on - strand at 2446.075 kb on + strand at 2446.084 kb on + strand at 2446.085 kb on - strand at 2446.085 kb on - strand at 2446.085 kb on - strand at 2446.085 kb on - strand at 2446.147 kb on + strand, within Pf6N2E2_2057 at 2446.147 kb on + strand, within Pf6N2E2_2057 at 2446.148 kb on - strand, within Pf6N2E2_2057 at 2446.733 kb on + strand, within Pf6N2E2_2057 at 2446.733 kb on + strand, within Pf6N2E2_2057 at 2446.910 kb on - strand, within Pf6N2E2_2057 at 2446.979 kb on - strand, within Pf6N2E2_2057 at 2446.979 kb on - strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.985 kb on - strand, within Pf6N2E2_2057 at 2446.985 kb on - strand, within Pf6N2E2_2057 at 2446.985 kb on - strand, within Pf6N2E2_2057 at 2446.985 kb on - strand, within Pf6N2E2_2057 at 2447.187 kb on + strand at 2447.188 kb on - strand, within Pf6N2E2_2057 at 2447.188 kb on - strand, within Pf6N2E2_2057 at 2447.188 kb on - strand, within Pf6N2E2_2057 at 2447.275 kb on + strand, within Pf6N2E2_2057 at 2447.275 kb on + strand, within Pf6N2E2_2057 at 2447.276 kb on - strand, within Pf6N2E2_2057 at 2447.276 kb on - strand, within Pf6N2E2_2057 at 2447.323 kb on + strand, within Pf6N2E2_2057 at 2447.323 kb on + strand, within Pf6N2E2_2057 at 2447.323 kb on + strand, within Pf6N2E2_2057 at 2447.517 kb on + strand at 2447.517 kb on + strand at 2447.537 kb on - strand at 2447.577 kb on + strand at 2447.616 kb on + strand at 2447.616 kb on + strand at 2447.617 kb on - strand at 2447.617 kb on - strand at 2447.617 kb on - strand at 2447.617 kb on - strand at 2447.635 kb on + strand at 2447.635 kb on + strand at 2447.635 kb on + strand at 2447.636 kb on - strand at 2447.636 kb on - strand at 2447.662 kb on + strand at 2447.663 kb on - strand at 2447.663 kb on - strand at 2447.671 kb on - strand at 2448.306 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 2,444,946 + +0.8 2,444,947 - +1.4 2,444,947 - -0.9 2,444,947 - +0.4 2,444,976 + Pf6N2E2_2054 0.13 +0.5 2,444,976 + Pf6N2E2_2054 0.13 +0.0 2,444,976 + Pf6N2E2_2054 0.13 +0.4 2,444,977 - Pf6N2E2_2054 0.13 +0.9 2,444,977 - Pf6N2E2_2054 0.13 +0.4 2,444,977 - Pf6N2E2_2054 0.13 -0.1 2,445,004 - Pf6N2E2_2054 0.21 +0.4 2,445,004 - Pf6N2E2_2054 0.21 -0.6 2,445,245 + +1.0 2,445,245 + +1.0 2,445,246 - +0.5 2,445,246 - +0.7 2,445,303 - -0.0 2,445,303 - +0.6 2,445,308 - +0.3 2,445,376 - +0.3 2,445,434 + Pf6N2E2_2055 0.29 +1.4 2,445,435 - Pf6N2E2_2055 0.29 +0.6 2,445,435 - Pf6N2E2_2055 0.29 +0.7 2,445,435 - Pf6N2E2_2055 0.29 -0.1 2,445,435 - Pf6N2E2_2055 0.29 +1.9 2,445,482 - Pf6N2E2_2055 0.46 +0.6 2,445,627 + +1.4 2,445,627 + +0.5 2,445,627 + +0.3 2,445,628 - -0.5 2,445,628 - +0.6 2,445,991 + +3.7 2,445,994 + +2.8 2,445,994 + +2.0 2,445,995 - +1.6 2,445,995 - +2.4 2,445,995 - +1.4 2,445,995 - +2.8 2,445,995 - +1.9 2,445,995 - +2.5 2,446,064 - +3.0 2,446,075 + +2.1 2,446,084 + +2.6 2,446,085 - +2.4 2,446,085 - +2.3 2,446,085 - +2.6 2,446,085 - +2.6 2,446,147 + Pf6N2E2_2057 0.13 +1.9 2,446,147 + Pf6N2E2_2057 0.13 +1.4 2,446,148 - Pf6N2E2_2057 0.13 +2.3 2,446,733 + Pf6N2E2_2057 0.48 +2.8 2,446,733 + Pf6N2E2_2057 0.48 +2.3 2,446,910 - Pf6N2E2_2057 0.59 +2.5 2,446,979 - Pf6N2E2_2057 0.63 +2.4 2,446,979 - Pf6N2E2_2057 0.63 +2.4 2,446,984 + Pf6N2E2_2057 0.63 +2.3 2,446,984 + Pf6N2E2_2057 0.63 +2.7 2,446,984 + Pf6N2E2_2057 0.63 +1.9 2,446,984 + Pf6N2E2_2057 0.63 +2.1 2,446,984 + Pf6N2E2_2057 0.63 +1.4 2,446,984 + Pf6N2E2_2057 0.63 +1.5 2,446,984 + Pf6N2E2_2057 0.63 +2.7 2,446,985 - Pf6N2E2_2057 0.64 +1.8 2,446,985 - Pf6N2E2_2057 0.64 +2.4 2,446,985 - Pf6N2E2_2057 0.64 +2.2 2,446,985 - Pf6N2E2_2057 0.64 +2.0 2,447,187 + +2.7 2,447,188 - Pf6N2E2_2057 0.76 +2.7 2,447,188 - Pf6N2E2_2057 0.76 +1.7 2,447,188 - Pf6N2E2_2057 0.76 +3.0 2,447,275 + Pf6N2E2_2057 0.81 +1.8 2,447,275 + Pf6N2E2_2057 0.81 +2.3 2,447,276 - Pf6N2E2_2057 0.81 +0.7 2,447,276 - Pf6N2E2_2057 0.81 +1.7 2,447,323 + Pf6N2E2_2057 0.84 +1.9 2,447,323 + Pf6N2E2_2057 0.84 +2.9 2,447,323 + Pf6N2E2_2057 0.84 +1.3 2,447,517 + +2.1 2,447,517 + +2.4 2,447,537 - +2.6 2,447,577 + +1.8 2,447,616 + +0.5 2,447,616 + +1.3 2,447,617 - +0.4 2,447,617 - -1.4 2,447,617 - -0.5 2,447,617 - +0.6 2,447,635 + -0.1 2,447,635 + +0.2 2,447,635 + +0.2 2,447,636 - +0.1 2,447,636 - -0.4 2,447,662 + +0.1 2,447,663 - +0.3 2,447,663 - -0.5 2,447,671 - -0.5 2,448,306 - -1.0
Or see this region's nucleotide sequence