Experiment: pyruvate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4726 and Pf1N1B4_4727 overlap by 4 nucleotides Pf1N1B4_4727 and Pf1N1B4_4728 are separated by 115 nucleotides
Pf1N1B4_4726 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 5,222,158 to 5,223,681
_4726
Pf1N1B4_4727 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 5,223,678 to 5,224,499
_4727
Pf1N1B4_4728 - Na+/H+ antiporter NhaB, at 5,224,615 to 5,226,117
_4728
Position (kb) 5223
5224
5225 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5222.746 kb on + strand, within Pf1N1B4_4726 at 5222.747 kb on - strand, within Pf1N1B4_4726 at 5222.943 kb on - strand, within Pf1N1B4_4726 at 5223.160 kb on + strand, within Pf1N1B4_4726 at 5223.160 kb on + strand, within Pf1N1B4_4726 at 5223.161 kb on - strand, within Pf1N1B4_4726 at 5223.215 kb on - strand, within Pf1N1B4_4726 at 5223.215 kb on - strand at 5223.215 kb on - strand, within Pf1N1B4_4726 at 5223.328 kb on + strand, within Pf1N1B4_4726 at 5223.329 kb on - strand, within Pf1N1B4_4726 at 5223.484 kb on + strand, within Pf1N1B4_4726 at 5223.484 kb on + strand at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.910 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.943 kb on - strand, within Pf1N1B4_4727 at 5224.119 kb on + strand at 5224.242 kb on + strand, within Pf1N1B4_4727 at 5224.242 kb on + strand, within Pf1N1B4_4727 at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.617 kb on - strand at 5224.617 kb on - strand at 5224.619 kb on - strand at 5224.626 kb on - strand at 5224.638 kb on - strand at 5224.665 kb on + strand at 5224.666 kb on - strand at 5224.666 kb on - strand at 5224.668 kb on - strand at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.930 kb on - strand, within Pf1N1B4_4728
Per-strain Table
Position Strand Gene LocusTag Fraction pyruvate (C) remove 5,222,746 + Pf1N1B4_4726 0.39 -0.8 5,222,747 - Pf1N1B4_4726 0.39 -1.5 5,222,943 - Pf1N1B4_4726 0.52 +0.1 5,223,160 + Pf1N1B4_4726 0.66 -0.6 5,223,160 + Pf1N1B4_4726 0.66 +0.7 5,223,161 - Pf1N1B4_4726 0.66 -0.3 5,223,215 - Pf1N1B4_4726 0.69 +0.5 5,223,215 - +0.1 5,223,215 - Pf1N1B4_4726 0.69 -2.8 5,223,328 + Pf1N1B4_4726 0.77 -0.6 5,223,329 - Pf1N1B4_4726 0.77 -0.9 5,223,484 + Pf1N1B4_4726 0.87 -0.0 5,223,484 + +1.3 5,223,485 - Pf1N1B4_4726 0.87 -0.0 5,223,485 - Pf1N1B4_4726 0.87 +0.4 5,223,485 - Pf1N1B4_4726 0.87 +0.3 5,223,485 - Pf1N1B4_4726 0.87 +0.7 5,223,485 - Pf1N1B4_4726 0.87 -0.9 5,223,910 + Pf1N1B4_4727 0.28 +1.3 5,223,942 + Pf1N1B4_4727 0.32 -0.1 5,223,942 + Pf1N1B4_4727 0.32 +1.2 5,223,942 + Pf1N1B4_4727 0.32 +0.3 5,223,942 + Pf1N1B4_4727 0.32 +0.7 5,223,943 - Pf1N1B4_4727 0.32 -0.5 5,224,119 + -0.6 5,224,242 + Pf1N1B4_4727 0.69 -1.9 5,224,242 + Pf1N1B4_4727 0.69 +0.0 5,224,482 + +0.3 5,224,482 + +0.1 5,224,482 + -0.0 5,224,482 + +0.7 5,224,483 - -0.9 5,224,483 - -0.6 5,224,483 - +0.3 5,224,483 - +0.2 5,224,483 - -0.6 5,224,483 - -0.0 5,224,617 - +0.9 5,224,617 - -0.3 5,224,619 - +0.7 5,224,626 - -0.0 5,224,638 - +0.3 5,224,665 + -1.9 5,224,666 - +0.7 5,224,666 - +1.3 5,224,668 - -0.5 5,224,875 - Pf1N1B4_4728 0.17 -1.9 5,224,875 - Pf1N1B4_4728 0.17 +0.6 5,224,875 - Pf1N1B4_4728 0.17 +0.3 5,224,875 - Pf1N1B4_4728 0.17 +1.9 5,224,930 - Pf1N1B4_4728 0.21 +0.1
Or see this region's nucleotide sequence