Experiment: pyruvate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_220 and Pf1N1B4_221 are separated by 12 nucleotides Pf1N1B4_221 and Pf1N1B4_222 are separated by 2 nucleotides Pf1N1B4_222 and Pf1N1B4_223 are separated by 134 nucleotides
Pf1N1B4_220 - 3-hydroxy-3isohexenylglutaryl-CoA:acetate lyase, at 279,272 to 281,074
_220
Pf1N1B4_221 - Citronellol and citronellal dehydrogenase, at 281,087 to 282,001
_221
Pf1N1B4_222 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 282,004 to 283,620
_222
Pf1N1B4_223 - Citronellyl-CoA dehydrogenase, at 283,755 to 284,912
_223
Position (kb) 282
283
284 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 281.073 kb on - strand at 281.073 kb on - strand at 281.073 kb on - strand at 281.073 kb on - strand at 281.073 kb on - strand at 281.093 kb on + strand at 281.093 kb on + strand at 281.394 kb on - strand, within Pf1N1B4_221 at 281.439 kb on + strand, within Pf1N1B4_221 at 281.439 kb on + strand, within Pf1N1B4_221 at 281.440 kb on - strand, within Pf1N1B4_221 at 281.693 kb on + strand, within Pf1N1B4_221 at 281.693 kb on + strand, within Pf1N1B4_221 at 281.693 kb on + strand at 281.693 kb on + strand, within Pf1N1B4_221 at 281.694 kb on - strand, within Pf1N1B4_221 at 281.694 kb on - strand, within Pf1N1B4_221 at 281.740 kb on - strand, within Pf1N1B4_221 at 281.740 kb on - strand, within Pf1N1B4_221 at 281.903 kb on + strand, within Pf1N1B4_221 at 281.904 kb on - strand, within Pf1N1B4_221 at 281.904 kb on - strand, within Pf1N1B4_221 at 281.924 kb on + strand at 281.925 kb on - strand at 281.925 kb on - strand at 281.928 kb on + strand at 281.937 kb on + strand at 281.938 kb on - strand at 282.181 kb on - strand, within Pf1N1B4_222 at 282.181 kb on - strand, within Pf1N1B4_222 at 282.463 kb on + strand, within Pf1N1B4_222 at 282.464 kb on - strand at 282.601 kb on + strand, within Pf1N1B4_222 at 282.602 kb on - strand, within Pf1N1B4_222 at 282.739 kb on + strand, within Pf1N1B4_222 at 282.739 kb on + strand, within Pf1N1B4_222 at 282.739 kb on + strand, within Pf1N1B4_222 at 283.224 kb on + strand, within Pf1N1B4_222 at 283.225 kb on - strand, within Pf1N1B4_222 at 283.252 kb on + strand, within Pf1N1B4_222 at 283.480 kb on + strand at 283.481 kb on - strand at 283.481 kb on - strand at 283.481 kb on - strand at 283.481 kb on - strand at 283.546 kb on + strand at 283.547 kb on - strand at 283.633 kb on - strand at 283.696 kb on + strand at 283.948 kb on - strand, within Pf1N1B4_223 at 283.948 kb on - strand, within Pf1N1B4_223 at 283.948 kb on - strand, within Pf1N1B4_223 at 283.954 kb on - strand, within Pf1N1B4_223 at 284.156 kb on - strand, within Pf1N1B4_223 at 284.196 kb on + strand, within Pf1N1B4_223 at 284.196 kb on + strand, within Pf1N1B4_223 at 284.197 kb on - strand at 284.197 kb on - strand, within Pf1N1B4_223 at 284.197 kb on - strand, within Pf1N1B4_223 at 284.332 kb on + strand, within Pf1N1B4_223 at 284.333 kb on - strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.520 kb on + strand, within Pf1N1B4_223 at 284.521 kb on - strand, within Pf1N1B4_223 at 284.544 kb on + strand, within Pf1N1B4_223
Per-strain Table
Position Strand Gene LocusTag Fraction pyruvate (C) remove 281,073 - +0.3 281,073 - -0.2 281,073 - -0.1 281,073 - -0.5 281,073 - -0.9 281,093 + +0.2 281,093 + +0.1 281,394 - Pf1N1B4_221 0.34 +0.1 281,439 + Pf1N1B4_221 0.38 +0.2 281,439 + Pf1N1B4_221 0.38 +0.3 281,440 - Pf1N1B4_221 0.39 +1.5 281,693 + Pf1N1B4_221 0.66 -0.1 281,693 + Pf1N1B4_221 0.66 -0.7 281,693 + -0.2 281,693 + Pf1N1B4_221 0.66 +1.5 281,694 - Pf1N1B4_221 0.66 +0.5 281,694 - Pf1N1B4_221 0.66 -0.0 281,740 - Pf1N1B4_221 0.71 +1.0 281,740 - Pf1N1B4_221 0.71 -1.1 281,903 + Pf1N1B4_221 0.89 -0.9 281,904 - Pf1N1B4_221 0.89 +0.8 281,904 - Pf1N1B4_221 0.89 +0.1 281,924 + -0.3 281,925 - +0.3 281,925 - +1.1 281,928 + +0.1 281,937 + +0.7 281,938 - +0.8 282,181 - Pf1N1B4_222 0.11 -0.5 282,181 - Pf1N1B4_222 0.11 -3.4 282,463 + Pf1N1B4_222 0.28 -0.3 282,464 - -0.9 282,601 + Pf1N1B4_222 0.37 -1.2 282,602 - Pf1N1B4_222 0.37 -1.3 282,739 + Pf1N1B4_222 0.45 -1.9 282,739 + Pf1N1B4_222 0.45 +1.1 282,739 + Pf1N1B4_222 0.45 -0.8 283,224 + Pf1N1B4_222 0.75 -1.4 283,225 - Pf1N1B4_222 0.76 -1.1 283,252 + Pf1N1B4_222 0.77 +0.4 283,480 + -0.5 283,481 - -2.1 283,481 - +0.1 283,481 - -0.2 283,481 - +0.3 283,546 + -1.0 283,547 - +0.2 283,633 - -0.5 283,696 + -2.5 283,948 - Pf1N1B4_223 0.17 +1.8 283,948 - Pf1N1B4_223 0.17 +0.1 283,948 - Pf1N1B4_223 0.17 +1.8 283,954 - Pf1N1B4_223 0.17 +1.3 284,156 - Pf1N1B4_223 0.35 +0.4 284,196 + Pf1N1B4_223 0.38 -1.5 284,196 + Pf1N1B4_223 0.38 +1.1 284,197 - -0.9 284,197 - Pf1N1B4_223 0.38 +2.1 284,197 - Pf1N1B4_223 0.38 -1.0 284,332 + Pf1N1B4_223 0.50 -1.4 284,333 - Pf1N1B4_223 0.50 -0.1 284,520 + Pf1N1B4_223 0.66 -0.9 284,520 + Pf1N1B4_223 0.66 +1.1 284,520 + Pf1N1B4_223 0.66 -1.2 284,520 + Pf1N1B4_223 0.66 +1.1 284,521 - Pf1N1B4_223 0.66 +0.9 284,544 + Pf1N1B4_223 0.68 +0.4
Or see this region's nucleotide sequence