Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32985 and RR42_RS32990 are separated by 153 nucleotides RR42_RS32990 and RR42_RS32995 are separated by 223 nucleotides RR42_RS32995 and RR42_RS33000 are separated by 43 nucleotides
RR42_RS32985: RR42_RS32985 - ABC transporter substrate-binding protein, at 2,801,502 to 2,802,491
_RS32985
RR42_RS32990: RR42_RS32990 - 1-aminocyclopropane-1-carboxylate deaminase, at 2,802,645 to 2,803,661
_RS32990
RR42_RS32995: RR42_RS32995 - AsnC family transcriptional regulator, at 2,803,885 to 2,804,394
_RS32995
RR42_RS33000: RR42_RS33000 - hypothetical protein, at 2,804,438 to 2,806,012
_RS33000
Position (kb)
2802
2803
2804 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2801.838 kb on - strand, within RR42_RS32985 at 2801.924 kb on - strand, within RR42_RS32985 at 2801.924 kb on - strand, within RR42_RS32985 at 2801.924 kb on - strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.930 kb on + strand, within RR42_RS32985 at 2801.931 kb on - strand, within RR42_RS32985 at 2801.931 kb on - strand, within RR42_RS32985 at 2801.931 kb on - strand, within RR42_RS32985 at 2801.931 kb on - strand, within RR42_RS32985 at 2802.016 kb on + strand, within RR42_RS32985 at 2802.017 kb on - strand, within RR42_RS32985 at 2802.212 kb on + strand, within RR42_RS32985 at 2802.212 kb on + strand, within RR42_RS32985 at 2802.212 kb on + strand, within RR42_RS32985 at 2802.213 kb on - strand, within RR42_RS32985 at 2802.213 kb on - strand, within RR42_RS32985 at 2802.213 kb on - strand, within RR42_RS32985 at 2802.213 kb on - strand, within RR42_RS32985 at 2802.213 kb on - strand, within RR42_RS32985 at 2802.215 kb on - strand, within RR42_RS32985 at 2802.215 kb on - strand, within RR42_RS32985 at 2802.496 kb on + strand at 2802.668 kb on + strand at 2802.668 kb on + strand at 2802.668 kb on + strand at 2802.669 kb on - strand at 2802.670 kb on + strand at 2802.707 kb on - strand at 2802.707 kb on - strand at 2802.707 kb on - strand at 2802.875 kb on - strand, within RR42_RS32990 at 2803.087 kb on + strand, within RR42_RS32990 at 2803.088 kb on - strand, within RR42_RS32990 at 2803.088 kb on - strand, within RR42_RS32990 at 2803.333 kb on + strand, within RR42_RS32990 at 2803.333 kb on + strand, within RR42_RS32990 at 2803.333 kb on + strand, within RR42_RS32990 at 2803.348 kb on + strand, within RR42_RS32990 at 2803.348 kb on + strand, within RR42_RS32990 at 2803.492 kb on + strand, within RR42_RS32990 at 2803.493 kb on - strand, within RR42_RS32990 at 2803.493 kb on - strand, within RR42_RS32990 at 2803.493 kb on - strand, within RR42_RS32990 at 2803.559 kb on - strand, within RR42_RS32990 at 2803.956 kb on + strand, within RR42_RS32995 at 2804.251 kb on + strand, within RR42_RS32995 at 2804.251 kb on + strand, within RR42_RS32995 at 2804.252 kb on - strand, within RR42_RS32995 at 2804.318 kb on - strand, within RR42_RS32995 at 2804.318 kb on - strand, within RR42_RS32995 at 2804.339 kb on + strand, within RR42_RS32995 at 2804.339 kb on + strand, within RR42_RS32995 at 2804.339 kb on + strand, within RR42_RS32995 at 2804.340 kb on - strand, within RR42_RS32995 at 2804.340 kb on - strand, within RR42_RS32995 at 2804.440 kb on - strand at 2804.536 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 2,801,838 - RR42_RS32985 0.34 +0.2 2,801,924 - RR42_RS32985 0.43 +0.6 2,801,924 - RR42_RS32985 0.43 -0.1 2,801,924 - RR42_RS32985 0.43 +0.6 2,801,930 + RR42_RS32985 0.43 +0.8 2,801,930 + RR42_RS32985 0.43 +0.2 2,801,930 + RR42_RS32985 0.43 -0.5 2,801,930 + RR42_RS32985 0.43 -0.1 2,801,930 + RR42_RS32985 0.43 +0.3 2,801,930 + RR42_RS32985 0.43 -0.4 2,801,931 - RR42_RS32985 0.43 -0.3 2,801,931 - RR42_RS32985 0.43 +1.5 2,801,931 - RR42_RS32985 0.43 +0.3 2,801,931 - RR42_RS32985 0.43 -0.2 2,802,016 + RR42_RS32985 0.52 -0.2 2,802,017 - RR42_RS32985 0.52 +0.4 2,802,212 + RR42_RS32985 0.72 +1.0 2,802,212 + RR42_RS32985 0.72 +1.0 2,802,212 + RR42_RS32985 0.72 +1.4 2,802,213 - RR42_RS32985 0.72 +0.1 2,802,213 - RR42_RS32985 0.72 -1.0 2,802,213 - RR42_RS32985 0.72 +0.4 2,802,213 - RR42_RS32985 0.72 +0.4 2,802,213 - RR42_RS32985 0.72 -0.1 2,802,215 - RR42_RS32985 0.72 +1.6 2,802,215 - RR42_RS32985 0.72 +0.5 2,802,496 + -0.2 2,802,668 + +0.5 2,802,668 + +0.4 2,802,668 + -1.8 2,802,669 - +0.3 2,802,670 + -0.4 2,802,707 - +0.1 2,802,707 - -0.9 2,802,707 - -0.6 2,802,875 - RR42_RS32990 0.23 +2.2 2,803,087 + RR42_RS32990 0.43 -0.5 2,803,088 - RR42_RS32990 0.44 -0.9 2,803,088 - RR42_RS32990 0.44 -0.1 2,803,333 + RR42_RS32990 0.68 -0.3 2,803,333 + RR42_RS32990 0.68 +0.6 2,803,333 + RR42_RS32990 0.68 +1.3 2,803,348 + RR42_RS32990 0.69 -0.5 2,803,348 + RR42_RS32990 0.69 +0.2 2,803,492 + RR42_RS32990 0.83 +1.0 2,803,493 - RR42_RS32990 0.83 +0.6 2,803,493 - RR42_RS32990 0.83 +0.4 2,803,493 - RR42_RS32990 0.83 +0.3 2,803,559 - RR42_RS32990 0.90 +0.6 2,803,956 + RR42_RS32995 0.14 -0.1 2,804,251 + RR42_RS32995 0.72 +0.8 2,804,251 + RR42_RS32995 0.72 -1.0 2,804,252 - RR42_RS32995 0.72 +1.8 2,804,318 - RR42_RS32995 0.85 +0.7 2,804,318 - RR42_RS32995 0.85 +0.3 2,804,339 + RR42_RS32995 0.89 +1.6 2,804,339 + RR42_RS32995 0.89 +1.2 2,804,339 + RR42_RS32995 0.89 -0.7 2,804,340 - RR42_RS32995 0.89 +0.9 2,804,340 - RR42_RS32995 0.89 -0.9 2,804,440 - -0.3 2,804,536 - -0.3
Or see this region's nucleotide sequence