Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27105 and RR42_RS27110 are separated by 45 nucleotides RR42_RS27110 and RR42_RS27115 are separated by 264 nucleotides RR42_RS27115 and RR42_RS27120 overlap by 20 nucleotides RR42_RS27120 and RR42_RS27125 overlap by 4 nucleotides
RR42_RS27105: RR42_RS27105 - hypothetical protein, at 1,435,127 to 1,435,360
_RS27105
RR42_RS27110: RR42_RS27110 - hypothetical protein, at 1,435,406 to 1,435,720
_RS27110
RR42_RS27115: RR42_RS27115 - amino acid ABC transporter ATP-binding protein, at 1,435,985 to 1,436,713
_RS27115
RR42_RS27120: RR42_RS27120 - amino acid ABC transporter permease, at 1,436,694 to 1,437,350
_RS27120
RR42_RS27125: RR42_RS27125 - polar amino acid ABC transporter permease, at 1,437,347 to 1,438,012
_RS27125
Position (kb)
1435
1436
1437 Strain fitness (log2 ratio)
-1
0
1 at 1435.060 kb on + strand at 1435.061 kb on - strand at 1435.139 kb on + strand at 1435.218 kb on + strand, within RR42_RS27105 at 1435.377 kb on + strand at 1435.377 kb on + strand at 1435.378 kb on - strand at 1435.521 kb on + strand, within RR42_RS27110 at 1435.522 kb on - strand, within RR42_RS27110 at 1435.522 kb on - strand, within RR42_RS27110 at 1435.522 kb on - strand, within RR42_RS27110 at 1435.697 kb on + strand at 1435.698 kb on - strand at 1435.791 kb on - strand at 1435.791 kb on - strand at 1435.801 kb on + strand at 1435.801 kb on + strand at 1435.825 kb on + strand at 1435.825 kb on + strand at 1435.826 kb on - strand at 1435.839 kb on + strand at 1435.839 kb on + strand at 1435.839 kb on + strand at 1435.840 kb on - strand at 1435.846 kb on - strand at 1436.231 kb on + strand, within RR42_RS27115 at 1436.232 kb on - strand, within RR42_RS27115 at 1436.232 kb on - strand, within RR42_RS27115 at 1436.232 kb on - strand, within RR42_RS27115 at 1436.232 kb on - strand, within RR42_RS27115 at 1436.536 kb on + strand, within RR42_RS27115 at 1436.569 kb on + strand, within RR42_RS27115 at 1436.954 kb on - strand, within RR42_RS27120 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.421 kb on - strand, within RR42_RS27125
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,435,060 + +0.2 1,435,061 - -0.5 1,435,139 + -0.3 1,435,218 + RR42_RS27105 0.39 +0.5 1,435,377 + -0.0 1,435,377 + +0.9 1,435,378 - +0.4 1,435,521 + RR42_RS27110 0.37 +0.4 1,435,522 - RR42_RS27110 0.37 -0.1 1,435,522 - RR42_RS27110 0.37 -0.3 1,435,522 - RR42_RS27110 0.37 -0.3 1,435,697 + -0.5 1,435,698 - -0.6 1,435,791 - +1.0 1,435,791 - +0.5 1,435,801 + -0.1 1,435,801 + +0.3 1,435,825 + -0.7 1,435,825 + +0.1 1,435,826 - +0.5 1,435,839 + +0.4 1,435,839 + -0.4 1,435,839 + -0.5 1,435,840 - -0.3 1,435,846 - +0.1 1,436,231 + RR42_RS27115 0.34 +0.6 1,436,232 - RR42_RS27115 0.34 +0.0 1,436,232 - RR42_RS27115 0.34 +0.1 1,436,232 - RR42_RS27115 0.34 +1.2 1,436,232 - RR42_RS27115 0.34 -0.3 1,436,536 + RR42_RS27115 0.76 -0.7 1,436,569 + RR42_RS27115 0.80 +0.5 1,436,954 - RR42_RS27120 0.40 -1.1 1,437,420 + RR42_RS27125 0.11 -1.7 1,437,420 + RR42_RS27125 0.11 +0.3 1,437,420 + RR42_RS27125 0.11 -0.0 1,437,421 - RR42_RS27125 0.11 +0.1
Or see this region's nucleotide sequence