Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22700 and RR42_RS22705 are separated by 506 nucleotides RR42_RS22705 and RR42_RS22710 are separated by 86 nucleotides RR42_RS22710 and RR42_RS22715 are separated by 225 nucleotides
RR42_RS22700: RR42_RS22700 - phasin, at 427,518 to 428,075
_RS22700
RR42_RS22705: RR42_RS22705 - oxidoreductase, at 428,582 to 428,905
_RS22705
RR42_RS22710: RR42_RS22710 - short-chain dehydrogenase, at 428,992 to 429,741
_RS22710
RR42_RS22715: RR42_RS22715 - AMP-binding protein, at 429,967 to 431,616
_RS22715
Position (kb) 428
429
430 Strain fitness (log2 ratio) -2
-1
0
1
2 at 428.000 kb on + strand, within RR42_RS22700 at 428.001 kb on - strand, within RR42_RS22700 at 428.001 kb on - strand, within RR42_RS22700 at 428.091 kb on + strand at 428.213 kb on + strand at 428.223 kb on + strand at 428.263 kb on + strand at 428.264 kb on - strand at 428.330 kb on + strand at 428.330 kb on + strand at 428.331 kb on - strand at 428.410 kb on - strand at 428.477 kb on - strand at 428.583 kb on + strand at 428.583 kb on + strand at 428.670 kb on + strand, within RR42_RS22705 at 428.695 kb on + strand, within RR42_RS22705 at 428.712 kb on + strand, within RR42_RS22705 at 428.712 kb on + strand, within RR42_RS22705 at 428.713 kb on - strand, within RR42_RS22705 at 428.752 kb on + strand, within RR42_RS22705 at 428.781 kb on + strand, within RR42_RS22705 at 428.782 kb on - strand, within RR42_RS22705 at 428.782 kb on - strand, within RR42_RS22705 at 428.782 kb on - strand, within RR42_RS22705 at 428.782 kb on - strand, within RR42_RS22705 at 428.885 kb on - strand at 428.940 kb on + strand at 428.940 kb on + strand at 428.940 kb on + strand at 428.941 kb on - strand at 428.941 kb on - strand at 428.941 kb on - strand at 428.949 kb on + strand at 428.950 kb on - strand at 428.966 kb on + strand at 428.967 kb on - strand at 428.967 kb on - strand at 429.180 kb on - strand, within RR42_RS22710 at 429.293 kb on + strand, within RR42_RS22710 at 429.782 kb on + strand at 429.782 kb on + strand at 429.782 kb on + strand at 429.801 kb on + strand at 429.968 kb on + strand at 429.969 kb on - strand at 430.080 kb on - strand at 430.148 kb on + strand, within RR42_RS22715 at 430.317 kb on + strand, within RR42_RS22715 at 430.367 kb on + strand, within RR42_RS22715 at 430.367 kb on + strand, within RR42_RS22715 at 430.368 kb on - strand, within RR42_RS22715 at 430.368 kb on - strand, within RR42_RS22715 at 430.370 kb on + strand, within RR42_RS22715 at 430.371 kb on - strand, within RR42_RS22715 at 430.441 kb on - strand, within RR42_RS22715 at 430.604 kb on + strand, within RR42_RS22715
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 428,000 + RR42_RS22700 0.86 +0.9 428,001 - RR42_RS22700 0.87 -0.4 428,001 - RR42_RS22700 0.87 +0.3 428,091 + -0.0 428,213 + +0.4 428,223 + -0.4 428,263 + +0.8 428,264 - +0.1 428,330 + -0.6 428,330 + -0.4 428,331 - +0.7 428,410 - +0.6 428,477 - -1.2 428,583 + -1.0 428,583 + +0.5 428,670 + RR42_RS22705 0.27 +0.1 428,695 + RR42_RS22705 0.35 -0.7 428,712 + RR42_RS22705 0.40 -0.2 428,712 + RR42_RS22705 0.40 -0.4 428,713 - RR42_RS22705 0.40 +1.3 428,752 + RR42_RS22705 0.52 -1.2 428,781 + RR42_RS22705 0.61 -0.4 428,782 - RR42_RS22705 0.62 +0.4 428,782 - RR42_RS22705 0.62 -0.0 428,782 - RR42_RS22705 0.62 +0.2 428,782 - RR42_RS22705 0.62 -1.4 428,885 - -0.4 428,940 + -0.6 428,940 + -1.0 428,940 + -1.2 428,941 - -0.8 428,941 - +0.7 428,941 - -1.0 428,949 + -1.0 428,950 - -1.1 428,966 + +0.1 428,967 - -1.0 428,967 - +0.1 429,180 - RR42_RS22710 0.25 -1.8 429,293 + RR42_RS22710 0.40 -0.3 429,782 + +0.8 429,782 + +2.1 429,782 + +1.4 429,801 + +0.3 429,968 + -0.5 429,969 - +0.7 430,080 - +0.7 430,148 + RR42_RS22715 0.11 +0.0 430,317 + RR42_RS22715 0.21 -2.1 430,367 + RR42_RS22715 0.24 -1.1 430,367 + RR42_RS22715 0.24 +1.1 430,368 - RR42_RS22715 0.24 -0.1 430,368 - RR42_RS22715 0.24 -1.6 430,370 + RR42_RS22715 0.24 -0.2 430,371 - RR42_RS22715 0.24 -0.3 430,441 - RR42_RS22715 0.29 +0.4 430,604 + RR42_RS22715 0.39 -0.4
Or see this region's nucleotide sequence