Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15465 and RR42_RS15470 are separated by 15 nucleotides RR42_RS15470 and RR42_RS15475 are separated by 42 nucleotides RR42_RS15475 and RR42_RS15480 overlap by 4 nucleotides RR42_RS15480 and RR42_RS15485 are separated by 385 nucleotides
RR42_RS15465: RR42_RS15465 - TonB-dependent receptor, at 3,341,582 to 3,342,736
_RS15465
RR42_RS15470: RR42_RS15470 - biopolymer transporter ExbD, at 3,342,752 to 3,343,189
_RS15470
RR42_RS15475: RR42_RS15475 - protein tolQ, at 3,343,232 to 3,343,915
_RS15475
RR42_RS15480: RR42_RS15480 - acyl-CoA thioesterase, at 3,343,912 to 3,344,316
_RS15480
RR42_RS15485: RR42_RS15485 - serine hydroxymethyltransferase, at 3,344,702 to 3,345,949
_RS15485
Position (kb)
3343
3344 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3342.512 kb on + strand, within RR42_RS15465 at 3342.634 kb on - strand at 3342.715 kb on - strand at 3343.122 kb on + strand, within RR42_RS15470 at 3343.122 kb on + strand, within RR42_RS15470 at 3343.464 kb on - strand, within RR42_RS15475 at 3343.486 kb on - strand, within RR42_RS15475 at 3343.486 kb on - strand, within RR42_RS15475 at 3343.684 kb on + strand, within RR42_RS15475 at 3343.786 kb on + strand, within RR42_RS15475 at 3343.795 kb on + strand, within RR42_RS15475 at 3343.795 kb on + strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.797 kb on + strand, within RR42_RS15475 at 3343.798 kb on - strand, within RR42_RS15475 at 3343.798 kb on - strand, within RR42_RS15475 at 3343.798 kb on - strand, within RR42_RS15475 at 3343.974 kb on + strand, within RR42_RS15480 at 3344.123 kb on + strand, within RR42_RS15480 at 3344.123 kb on + strand, within RR42_RS15480 at 3344.123 kb on + strand, within RR42_RS15480 at 3344.124 kb on - strand, within RR42_RS15480 at 3344.124 kb on - strand, within RR42_RS15480 at 3344.241 kb on - strand, within RR42_RS15480 at 3344.257 kb on + strand, within RR42_RS15480 at 3344.258 kb on - strand, within RR42_RS15480 at 3344.286 kb on - strand at 3344.329 kb on - strand at 3344.371 kb on + strand at 3344.371 kb on + strand at 3344.371 kb on + strand at 3344.372 kb on - strand at 3344.380 kb on + strand at 3344.380 kb on + strand at 3344.381 kb on - strand at 3344.381 kb on - strand at 3344.512 kb on + strand at 3344.608 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,342,512 + RR42_RS15465 0.81 -2.4 3,342,634 - -1.9 3,342,715 - -0.6 3,343,122 + RR42_RS15470 0.84 -1.6 3,343,122 + RR42_RS15470 0.84 -2.0 3,343,464 - RR42_RS15475 0.34 -2.8 3,343,486 - RR42_RS15475 0.37 -2.6 3,343,486 - RR42_RS15475 0.37 -2.4 3,343,684 + RR42_RS15475 0.66 -2.2 3,343,786 + RR42_RS15475 0.81 -0.7 3,343,795 + RR42_RS15475 0.82 +0.6 3,343,795 + RR42_RS15475 0.82 -3.0 3,343,796 - RR42_RS15475 0.82 -0.8 3,343,796 - RR42_RS15475 0.82 -2.6 3,343,796 - RR42_RS15475 0.82 -0.9 3,343,796 - RR42_RS15475 0.82 -1.1 3,343,796 - RR42_RS15475 0.82 -1.1 3,343,796 - RR42_RS15475 0.82 -1.8 3,343,797 + RR42_RS15475 0.83 -0.0 3,343,798 - RR42_RS15475 0.83 +0.8 3,343,798 - RR42_RS15475 0.83 -0.6 3,343,798 - RR42_RS15475 0.83 -2.3 3,343,974 + RR42_RS15480 0.15 -1.8 3,344,123 + RR42_RS15480 0.52 -0.4 3,344,123 + RR42_RS15480 0.52 +0.6 3,344,123 + RR42_RS15480 0.52 -0.7 3,344,124 - RR42_RS15480 0.52 +0.5 3,344,124 - RR42_RS15480 0.52 -0.0 3,344,241 - RR42_RS15480 0.81 +0.6 3,344,257 + RR42_RS15480 0.85 -0.6 3,344,258 - RR42_RS15480 0.85 -2.1 3,344,286 - +0.1 3,344,329 - +1.2 3,344,371 + +0.8 3,344,371 + -0.3 3,344,371 + -0.0 3,344,372 - +1.1 3,344,380 + -0.4 3,344,380 + -0.9 3,344,381 - +0.1 3,344,381 - +1.6 3,344,512 + +0.5 3,344,608 + +1.3
Or see this region's nucleotide sequence