Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15345 and RR42_RS15350 overlap by 4 nucleotides RR42_RS15350 and RR42_RS15355 are separated by 58 nucleotides RR42_RS15355 and RR42_RS15360 are separated by 8 nucleotides
RR42_RS15345: RR42_RS15345 - long-chain fatty acid--CoA ligase, at 3,318,959 to 3,320,512
_RS15345
RR42_RS15350: RR42_RS15350 - BEC protein, at 3,320,509 to 3,321,753
_RS15350
RR42_RS15355: RR42_RS15355 - alpha/beta hydrolase, at 3,321,812 to 3,322,642
_RS15355
RR42_RS15360: RR42_RS15360 - peptidase M29, at 3,322,651 to 3,323,757
_RS15360
Position (kb)
3320
3321
3322 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.620 kb on + strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.621 kb on - strand, within RR42_RS15345 at 3319.992 kb on + strand, within RR42_RS15345 at 3319.992 kb on + strand, within RR42_RS15345 at 3319.992 kb on + strand, within RR42_RS15345 at 3319.992 kb on + strand, within RR42_RS15345 at 3319.993 kb on - strand, within RR42_RS15345 at 3319.993 kb on - strand, within RR42_RS15345 at 3319.993 kb on - strand, within RR42_RS15345 at 3319.993 kb on - strand, within RR42_RS15345 at 3320.382 kb on + strand at 3320.382 kb on + strand at 3320.383 kb on - strand at 3320.392 kb on + strand at 3320.392 kb on + strand at 3320.393 kb on - strand at 3320.404 kb on + strand at 3320.404 kb on + strand at 3320.404 kb on + strand at 3320.641 kb on + strand, within RR42_RS15350 at 3320.711 kb on + strand, within RR42_RS15350 at 3320.711 kb on + strand, within RR42_RS15350 at 3320.711 kb on - strand, within RR42_RS15350 at 3320.847 kb on - strand, within RR42_RS15350 at 3320.912 kb on + strand, within RR42_RS15350 at 3320.912 kb on + strand, within RR42_RS15350 at 3320.937 kb on - strand, within RR42_RS15350 at 3320.987 kb on + strand, within RR42_RS15350 at 3320.987 kb on + strand, within RR42_RS15350 at 3320.988 kb on - strand, within RR42_RS15350 at 3320.988 kb on - strand, within RR42_RS15350 at 3320.991 kb on - strand, within RR42_RS15350 at 3320.991 kb on - strand, within RR42_RS15350 at 3321.134 kb on + strand, within RR42_RS15350 at 3321.134 kb on + strand, within RR42_RS15350 at 3321.134 kb on + strand, within RR42_RS15350 at 3321.135 kb on - strand, within RR42_RS15350 at 3321.194 kb on + strand, within RR42_RS15350 at 3321.195 kb on - strand, within RR42_RS15350 at 3321.198 kb on - strand, within RR42_RS15350 at 3321.332 kb on + strand, within RR42_RS15350 at 3321.332 kb on + strand, within RR42_RS15350 at 3321.333 kb on - strand, within RR42_RS15350 at 3321.333 kb on - strand, within RR42_RS15350 at 3321.333 kb on - strand, within RR42_RS15350 at 3321.521 kb on + strand, within RR42_RS15350 at 3321.521 kb on + strand, within RR42_RS15350 at 3321.521 kb on + strand, within RR42_RS15350 at 3321.522 kb on - strand, within RR42_RS15350 at 3321.522 kb on - strand, within RR42_RS15350 at 3321.522 kb on - strand, within RR42_RS15350 at 3321.602 kb on + strand, within RR42_RS15350 at 3321.603 kb on - strand, within RR42_RS15350 at 3321.603 kb on - strand, within RR42_RS15350 at 3321.603 kb on - strand, within RR42_RS15350 at 3322.068 kb on + strand, within RR42_RS15355 at 3322.308 kb on + strand, within RR42_RS15355 at 3322.308 kb on + strand, within RR42_RS15355 at 3322.309 kb on - strand, within RR42_RS15355 at 3322.347 kb on - strand, within RR42_RS15355 at 3322.347 kb on - strand, within RR42_RS15355
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,319,620 + RR42_RS15345 0.43 +0.2 3,319,620 + RR42_RS15345 0.43 -0.2 3,319,620 + RR42_RS15345 0.43 +0.4 3,319,620 + RR42_RS15345 0.43 -0.2 3,319,621 - RR42_RS15345 0.43 +0.5 3,319,621 - RR42_RS15345 0.43 +1.8 3,319,621 - RR42_RS15345 0.43 +0.5 3,319,621 - RR42_RS15345 0.43 +0.5 3,319,621 - RR42_RS15345 0.43 +0.1 3,319,992 + RR42_RS15345 0.66 +0.4 3,319,992 + RR42_RS15345 0.66 +0.3 3,319,992 + RR42_RS15345 0.66 +1.0 3,319,992 + RR42_RS15345 0.66 +1.0 3,319,993 - RR42_RS15345 0.67 +0.1 3,319,993 - RR42_RS15345 0.67 -0.5 3,319,993 - RR42_RS15345 0.67 -0.8 3,319,993 - RR42_RS15345 0.67 -0.2 3,320,382 + -1.8 3,320,382 + -0.5 3,320,383 - -0.5 3,320,392 + -0.2 3,320,392 + +3.8 3,320,393 - -1.6 3,320,404 + +0.0 3,320,404 + -2.0 3,320,404 + +0.3 3,320,641 + RR42_RS15350 0.11 +0.5 3,320,711 + RR42_RS15350 0.16 -0.1 3,320,711 + RR42_RS15350 0.16 -0.9 3,320,711 - RR42_RS15350 0.16 -1.4 3,320,847 - RR42_RS15350 0.27 +0.5 3,320,912 + RR42_RS15350 0.32 +0.5 3,320,912 + RR42_RS15350 0.32 -0.6 3,320,937 - RR42_RS15350 0.34 -0.1 3,320,987 + RR42_RS15350 0.38 -0.3 3,320,987 + RR42_RS15350 0.38 -2.2 3,320,988 - RR42_RS15350 0.38 -1.6 3,320,988 - RR42_RS15350 0.38 +2.0 3,320,991 - RR42_RS15350 0.39 +0.6 3,320,991 - RR42_RS15350 0.39 -0.2 3,321,134 + RR42_RS15350 0.50 -0.5 3,321,134 + RR42_RS15350 0.50 -1.7 3,321,134 + RR42_RS15350 0.50 -0.2 3,321,135 - RR42_RS15350 0.50 +0.3 3,321,194 + RR42_RS15350 0.55 -0.3 3,321,195 - RR42_RS15350 0.55 +0.5 3,321,198 - RR42_RS15350 0.55 +0.0 3,321,332 + RR42_RS15350 0.66 -0.5 3,321,332 + RR42_RS15350 0.66 +0.5 3,321,333 - RR42_RS15350 0.66 -0.0 3,321,333 - RR42_RS15350 0.66 -0.0 3,321,333 - RR42_RS15350 0.66 +1.0 3,321,521 + RR42_RS15350 0.81 -0.4 3,321,521 + RR42_RS15350 0.81 -0.5 3,321,521 + RR42_RS15350 0.81 +0.2 3,321,522 - RR42_RS15350 0.81 -0.0 3,321,522 - RR42_RS15350 0.81 +0.4 3,321,522 - RR42_RS15350 0.81 +0.3 3,321,602 + RR42_RS15350 0.88 -0.4 3,321,603 - RR42_RS15350 0.88 +0.3 3,321,603 - RR42_RS15350 0.88 -0.6 3,321,603 - RR42_RS15350 0.88 +0.1 3,322,068 + RR42_RS15355 0.31 +0.1 3,322,308 + RR42_RS15355 0.60 -0.5 3,322,308 + RR42_RS15355 0.60 -1.4 3,322,309 - RR42_RS15355 0.60 -0.3 3,322,347 - RR42_RS15355 0.64 -0.9 3,322,347 - RR42_RS15355 0.64 +0.5
Or see this region's nucleotide sequence