Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15215 and RR42_RS15220 are separated by 214 nucleotides RR42_RS15220 and RR42_RS15225 are separated by 4 nucleotides RR42_RS15225 and RR42_RS15230 are separated by 79 nucleotides RR42_RS15230 and RR42_RS15235 are separated by 11 nucleotides
RR42_RS15215: RR42_RS15215 - carnitine dehydratase, at 3,295,254 to 3,296,693
_RS15215
RR42_RS15220: RR42_RS15220 - glutaminyl-tRNA synthetase, at 3,296,908 to 3,298,686
_RS15220
RR42_RS15225: RR42_RS15225 - RNA-binding protein, at 3,298,691 to 3,298,909
_RS15225
RR42_RS15230: RR42_RS15230 - membrane protein, at 3,298,989 to 3,299,372
_RS15230
RR42_RS15235: RR42_RS15235 - hypothetical protein, at 3,299,384 to 3,299,986
_RS15235
Position (kb) 3296
3297
3298
3299 Strain fitness (log2 ratio) -1
0
1 at 3296.329 kb on - strand, within RR42_RS15215 at 3296.410 kb on + strand, within RR42_RS15215 at 3296.678 kb on + strand at 3296.678 kb on + strand at 3296.734 kb on + strand at 3296.735 kb on - strand at 3298.685 kb on - strand at 3298.685 kb on - strand at 3298.726 kb on + strand, within RR42_RS15225 at 3299.265 kb on + strand, within RR42_RS15230 at 3299.266 kb on - strand, within RR42_RS15230 at 3299.266 kb on - strand, within RR42_RS15230 at 3299.277 kb on + strand, within RR42_RS15230 at 3299.277 kb on + strand, within RR42_RS15230 at 3299.343 kb on - strand at 3299.343 kb on - strand at 3299.498 kb on + strand, within RR42_RS15235 at 3299.498 kb on + strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,296,329 - RR42_RS15215 0.75 +0.4 3,296,410 + RR42_RS15215 0.80 +0.9 3,296,678 + -0.1 3,296,678 + +0.3 3,296,734 + +0.1 3,296,735 - -1.4 3,298,685 - -0.9 3,298,685 - +0.8 3,298,726 + RR42_RS15225 0.16 -1.1 3,299,265 + RR42_RS15230 0.72 -1.3 3,299,266 - RR42_RS15230 0.72 -0.9 3,299,266 - RR42_RS15230 0.72 +0.4 3,299,277 + RR42_RS15230 0.75 -0.4 3,299,277 + RR42_RS15230 0.75 -0.5 3,299,343 - -1.2 3,299,343 - -0.4 3,299,498 + RR42_RS15235 0.19 -1.1 3,299,498 + RR42_RS15235 0.19 -0.9 3,299,499 - RR42_RS15235 0.19 -0.7 3,299,499 - RR42_RS15235 0.19 -0.0 3,299,499 - RR42_RS15235 0.19 +0.8
Or see this region's nucleotide sequence