Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04040 and RR42_RS04045 are separated by 122 nucleotides RR42_RS04045 and RR42_RS04050 are separated by 48 nucleotides RR42_RS04050 and RR42_RS04055 are separated by 255 nucleotides
RR42_RS04040: RR42_RS04040 - hypothetical protein, at 896,239 to 896,526
_RS04040
RR42_RS04045: RR42_RS04045 - proline hydroxylase, at 896,649 to 897,257
_RS04045
RR42_RS04050: RR42_RS04050 - Crp/Fnr family transcriptional regulator, at 897,306 to 898,028
_RS04050
RR42_RS04055: RR42_RS04055 - hypothetical protein, at 898,284 to 898,745
_RS04055
Position (kb)
897
898
899 Strain fitness (log2 ratio)
-1
0
1 at 896.459 kb on - strand, within RR42_RS04040 at 896.459 kb on - strand, within RR42_RS04040 at 896.459 kb on - strand, within RR42_RS04040 at 896.459 kb on - strand, within RR42_RS04040 at 896.500 kb on + strand at 896.501 kb on - strand at 896.561 kb on + strand at 896.561 kb on + strand at 896.561 kb on + strand at 896.995 kb on - strand, within RR42_RS04045 at 896.995 kb on - strand, within RR42_RS04045 at 897.196 kb on + strand, within RR42_RS04045 at 897.196 kb on + strand, within RR42_RS04045 at 897.197 kb on - strand at 897.282 kb on + strand at 897.433 kb on - strand, within RR42_RS04050 at 897.881 kb on + strand, within RR42_RS04050 at 897.973 kb on + strand at 898.026 kb on + strand at 898.027 kb on - strand at 898.063 kb on - strand at 898.242 kb on - strand at 898.243 kb on + strand at 898.243 kb on + strand at 898.545 kb on + strand, within RR42_RS04055 at 898.546 kb on - strand, within RR42_RS04055 at 898.778 kb on + strand at 898.779 kb on - strand at 898.779 kb on - strand at 898.820 kb on + strand at 898.821 kb on - strand at 898.821 kb on - strand at 898.859 kb on + strand at 898.875 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 896,459 - RR42_RS04040 0.76 +0.1 896,459 - RR42_RS04040 0.76 +0.8 896,459 - RR42_RS04040 0.76 -0.2 896,459 - RR42_RS04040 0.76 -1.2 896,500 + +0.2 896,501 - -0.5 896,561 + -0.4 896,561 + -0.4 896,561 + +0.9 896,995 - RR42_RS04045 0.57 -0.7 896,995 - RR42_RS04045 0.57 -0.4 897,196 + RR42_RS04045 0.90 -0.5 897,196 + RR42_RS04045 0.90 +1.2 897,197 - -0.3 897,282 + +0.1 897,433 - RR42_RS04050 0.18 +0.4 897,881 + RR42_RS04050 0.80 -1.1 897,973 + -0.5 898,026 + +0.1 898,027 - +0.4 898,063 - +0.8 898,242 - -0.3 898,243 + +0.2 898,243 + +0.2 898,545 + RR42_RS04055 0.56 -0.6 898,546 - RR42_RS04055 0.57 -0.7 898,778 + +1.1 898,779 - +1.3 898,779 - -0.3 898,820 + +1.0 898,821 - +0.1 898,821 - +0.8 898,859 + -0.0 898,875 + -1.0
Or see this region's nucleotide sequence