Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03245 and RR42_RS03250 are separated by 138 nucleotides RR42_RS03250 and RR42_RS03255 are separated by 39 nucleotides RR42_RS03255 and RR42_RS03260 overlap by 4 nucleotides
RR42_RS03245: RR42_RS03245 - D-alanyl-D-alanine carboxypeptidase, at 719,185 to 720,717
_RS03245
RR42_RS03250: RR42_RS03250 - MarR family transcriptional regulator, at 720,856 to 721,416
_RS03250
RR42_RS03255: RR42_RS03255 - hypothetical protein, at 721,456 to 721,956
_RS03255
RR42_RS03260: RR42_RS03260 - branched-chain amino acid ABC transporter substrate-binding protein, at 721,953 to 723,071
_RS03260
Position (kb) 720
721
722 Strain fitness (log2 ratio) -2
-1
0
1
2 at 719.888 kb on + strand, within RR42_RS03245 at 719.888 kb on + strand, within RR42_RS03245 at 720.073 kb on - strand, within RR42_RS03245 at 720.095 kb on + strand, within RR42_RS03245 at 720.096 kb on - strand, within RR42_RS03245 at 720.122 kb on + strand, within RR42_RS03245 at 720.123 kb on - strand, within RR42_RS03245 at 720.179 kb on + strand, within RR42_RS03245 at 720.179 kb on + strand, within RR42_RS03245 at 720.296 kb on + strand, within RR42_RS03245 at 720.296 kb on + strand, within RR42_RS03245 at 720.344 kb on + strand, within RR42_RS03245 at 720.345 kb on - strand, within RR42_RS03245 at 720.488 kb on + strand, within RR42_RS03245 at 720.489 kb on - strand, within RR42_RS03245 at 720.721 kb on - strand at 721.007 kb on + strand, within RR42_RS03250 at 721.218 kb on - strand, within RR42_RS03250 at 721.218 kb on - strand, within RR42_RS03250 at 721.221 kb on + strand, within RR42_RS03250 at 721.457 kb on + strand at 721.458 kb on - strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.459 kb on + strand at 721.460 kb on - strand at 721.583 kb on + strand, within RR42_RS03255 at 721.583 kb on + strand, within RR42_RS03255 at 721.583 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.670 kb on + strand, within RR42_RS03255 at 721.671 kb on - strand, within RR42_RS03255 at 721.703 kb on + strand, within RR42_RS03255 at 721.703 kb on + strand, within RR42_RS03255 at 722.038 kb on + strand at 722.039 kb on - strand at 722.067 kb on - strand, within RR42_RS03260 at 722.067 kb on - strand, within RR42_RS03260 at 722.180 kb on - strand, within RR42_RS03260 at 722.180 kb on - strand, within RR42_RS03260 at 722.219 kb on - strand, within RR42_RS03260 at 722.242 kb on + strand, within RR42_RS03260 at 722.384 kb on - strand, within RR42_RS03260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 719,888 + RR42_RS03245 0.46 -0.4 719,888 + RR42_RS03245 0.46 +0.3 720,073 - RR42_RS03245 0.58 -1.4 720,095 + RR42_RS03245 0.59 -0.2 720,096 - RR42_RS03245 0.59 +0.3 720,122 + RR42_RS03245 0.61 +0.5 720,123 - RR42_RS03245 0.61 -1.9 720,179 + RR42_RS03245 0.65 -1.1 720,179 + RR42_RS03245 0.65 +0.2 720,296 + RR42_RS03245 0.72 -0.4 720,296 + RR42_RS03245 0.72 -1.3 720,344 + RR42_RS03245 0.76 -0.7 720,345 - RR42_RS03245 0.76 -0.3 720,488 + RR42_RS03245 0.85 -2.3 720,489 - RR42_RS03245 0.85 +2.0 720,721 - -0.4 721,007 + RR42_RS03250 0.27 +0.0 721,218 - RR42_RS03250 0.65 +0.9 721,218 - RR42_RS03250 0.65 -0.2 721,221 + RR42_RS03250 0.65 +0.6 721,457 + -0.0 721,458 - -0.6 721,459 + -1.2 721,459 + +0.2 721,459 + +0.5 721,459 + -0.9 721,459 + +1.1 721,460 - +0.1 721,583 + RR42_RS03255 0.25 -0.2 721,583 + RR42_RS03255 0.25 -0.4 721,583 + RR42_RS03255 0.25 +0.5 721,670 + RR42_RS03255 0.43 +0.8 721,670 + RR42_RS03255 0.43 +0.9 721,670 + RR42_RS03255 0.43 +0.8 721,670 + RR42_RS03255 0.43 -2.3 721,671 - RR42_RS03255 0.43 +1.2 721,703 + RR42_RS03255 0.49 +0.4 721,703 + RR42_RS03255 0.49 +0.3 722,038 + +0.5 722,039 - -0.1 722,067 - RR42_RS03260 0.10 -0.4 722,067 - RR42_RS03260 0.10 -0.5 722,180 - RR42_RS03260 0.20 +0.5 722,180 - RR42_RS03260 0.20 -0.5 722,219 - RR42_RS03260 0.24 -1.1 722,242 + RR42_RS03260 0.26 +0.0 722,384 - RR42_RS03260 0.39 -0.2
Or see this region's nucleotide sequence