Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02395 and RR42_RS02400 are separated by 42 nucleotides RR42_RS02400 and RR42_RS02405 are separated by 76 nucleotides RR42_RS02405 and RR42_RS02410 are separated by 210 nucleotides RR42_RS02410 and RR42_RS02415 are separated by 105 nucleotides
RR42_RS02395: RR42_RS02395 - hypothetical protein, at 540,139 to 540,552
_RS02395
RR42_RS02400: RR42_RS02400 - ubiquinone biosynthesis methyltransferase UbiE, at 540,595 to 541,326
_RS02400
RR42_RS02405: RR42_RS02405 - membrane protein, at 541,403 to 542,404
_RS02405
RR42_RS02410: RR42_RS02410 - sterol-binding protein, at 542,615 to 543,289
_RS02410
RR42_RS02415: RR42_RS02415 - ubiquinone biosynthesis protein UbiB, at 543,395 to 544,972
_RS02415
Position (kb)
541
542
543 Strain fitness (log2 ratio)
-2
-1
0
1 at 540.412 kb on + strand, within RR42_RS02395 at 540.470 kb on - strand, within RR42_RS02395 at 541.332 kb on + strand at 541.335 kb on - strand at 541.378 kb on + strand at 541.633 kb on + strand, within RR42_RS02405 at 541.634 kb on - strand, within RR42_RS02405 at 542.078 kb on + strand, within RR42_RS02405 at 542.078 kb on + strand, within RR42_RS02405 at 542.078 kb on + strand, within RR42_RS02405 at 542.078 kb on + strand, within RR42_RS02405 at 542.079 kb on - strand, within RR42_RS02405 at 542.081 kb on + strand, within RR42_RS02405 at 542.081 kb on + strand, within RR42_RS02405 at 542.081 kb on + strand, within RR42_RS02405 at 542.081 kb on + strand, within RR42_RS02405 at 542.082 kb on - strand, within RR42_RS02405 at 542.082 kb on - strand, within RR42_RS02405 at 542.082 kb on - strand, within RR42_RS02405 at 542.085 kb on + strand, within RR42_RS02405 at 542.085 kb on + strand, within RR42_RS02405 at 542.086 kb on - strand, within RR42_RS02405 at 542.086 kb on - strand, within RR42_RS02405 at 542.432 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.450 kb on + strand at 542.451 kb on - strand at 542.451 kb on - strand at 542.451 kb on - strand at 542.522 kb on + strand at 542.522 kb on + strand at 542.526 kb on + strand at 542.582 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 540,412 + RR42_RS02395 0.66 -0.2 540,470 - RR42_RS02395 0.80 -0.8 541,332 + -0.2 541,335 - -0.8 541,378 + -0.0 541,633 + RR42_RS02405 0.23 +0.2 541,634 - RR42_RS02405 0.23 -0.9 542,078 + RR42_RS02405 0.67 -0.1 542,078 + RR42_RS02405 0.67 -1.5 542,078 + RR42_RS02405 0.67 +0.3 542,078 + RR42_RS02405 0.67 +0.4 542,079 - RR42_RS02405 0.67 +0.3 542,081 + RR42_RS02405 0.68 +0.5 542,081 + RR42_RS02405 0.68 -1.3 542,081 + RR42_RS02405 0.68 -0.9 542,081 + RR42_RS02405 0.68 +0.3 542,082 - RR42_RS02405 0.68 +0.2 542,082 - RR42_RS02405 0.68 -0.7 542,082 - RR42_RS02405 0.68 +0.1 542,085 + RR42_RS02405 0.68 -0.6 542,085 + RR42_RS02405 0.68 -0.5 542,086 - RR42_RS02405 0.68 -0.2 542,086 - RR42_RS02405 0.68 -0.2 542,432 + +0.4 542,450 + -0.1 542,450 + -0.6 542,450 + +0.5 542,450 + +0.2 542,450 + +0.1 542,450 + -0.6 542,450 + +0.5 542,450 + +0.6 542,450 + +1.1 542,450 + +0.6 542,450 + +0.6 542,451 - +0.6 542,451 - +0.3 542,451 - +0.7 542,522 + +0.2 542,522 + -2.0 542,526 + +0.0 542,582 + -0.4
Or see this region's nucleotide sequence