Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01170 and RR42_RS01175 are separated by 121 nucleotides RR42_RS01175 and RR42_RS01180 are separated by 100 nucleotides
RR42_RS01170: RR42_RS01170 - LysR family transcriptional regulator, at 268,200 to 269,117
_RS01170
RR42_RS01175: RR42_RS01175 - membrane protein, at 269,239 to 270,183
_RS01175
RR42_RS01180: RR42_RS01180 - acyl-CoA thioesterase, at 270,284 to 271,114
_RS01180
Position (kb)
269
270
271 Strain fitness (log2 ratio)
-2
-1
0
1 at 268.245 kb on - strand at 268.285 kb on + strand at 268.286 kb on - strand at 268.305 kb on + strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.306 kb on - strand, within RR42_RS01170 at 268.540 kb on + strand, within RR42_RS01170 at 268.540 kb on + strand, within RR42_RS01170 at 268.541 kb on - strand, within RR42_RS01170 at 268.541 kb on - strand, within RR42_RS01170 at 269.085 kb on + strand at 269.086 kb on - strand at 269.131 kb on + strand at 269.132 kb on - strand at 269.142 kb on - strand at 269.152 kb on + strand at 269.215 kb on + strand at 269.216 kb on - strand at 269.216 kb on - strand at 269.216 kb on - strand at 269.227 kb on + strand at 269.228 kb on - strand at 269.228 kb on - strand at 269.523 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.566 kb on + strand, within RR42_RS01175 at 269.567 kb on - strand, within RR42_RS01175 at 269.596 kb on + strand, within RR42_RS01175 at 269.785 kb on + strand, within RR42_RS01175 at 269.956 kb on - strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.968 kb on + strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.969 kb on - strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.980 kb on + strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 269.981 kb on - strand, within RR42_RS01175 at 270.034 kb on + strand, within RR42_RS01175 at 270.387 kb on - strand, within RR42_RS01180 at 270.737 kb on + strand, within RR42_RS01180 at 270.737 kb on + strand, within RR42_RS01180 at 270.737 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.944 kb on + strand, within RR42_RS01180 at 270.945 kb on - strand, within RR42_RS01180 at 271.103 kb on + strand at 271.103 kb on + strand at 271.103 kb on + strand at 271.103 kb on + strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.104 kb on - strand at 271.112 kb on + strand at 271.112 kb on + strand at 271.112 kb on + strand at 271.113 kb on - strand at 271.113 kb on - strand at 271.113 kb on - strand at 271.171 kb on - strand at 271.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 268,245 - -0.6 268,285 + +0.2 268,286 - -0.7 268,305 + RR42_RS01170 0.11 +0.9 268,306 - RR42_RS01170 0.12 +0.3 268,306 - RR42_RS01170 0.12 +0.1 268,306 - RR42_RS01170 0.12 +1.0 268,306 - RR42_RS01170 0.12 +0.6 268,540 + RR42_RS01170 0.37 +0.0 268,540 + RR42_RS01170 0.37 +1.6 268,541 - RR42_RS01170 0.37 +1.2 268,541 - RR42_RS01170 0.37 +0.3 269,085 + -1.2 269,086 - +0.0 269,131 + +0.8 269,132 - -0.2 269,142 - +0.5 269,152 + +0.5 269,215 + -0.2 269,216 - +0.2 269,216 - +1.0 269,216 - -0.5 269,227 + -0.1 269,228 - +0.7 269,228 - -1.1 269,523 + RR42_RS01175 0.30 +0.8 269,566 + RR42_RS01175 0.35 -1.8 269,566 + RR42_RS01175 0.35 +0.8 269,566 + RR42_RS01175 0.35 -0.2 269,567 - RR42_RS01175 0.35 +0.5 269,596 + RR42_RS01175 0.38 -0.0 269,785 + RR42_RS01175 0.58 +1.3 269,956 - RR42_RS01175 0.76 -0.2 269,968 + RR42_RS01175 0.77 -0.1 269,968 + RR42_RS01175 0.77 +0.6 269,968 + RR42_RS01175 0.77 -0.6 269,968 + RR42_RS01175 0.77 -0.3 269,969 - RR42_RS01175 0.77 -0.3 269,969 - RR42_RS01175 0.77 -0.4 269,969 - RR42_RS01175 0.77 +0.2 269,969 - RR42_RS01175 0.77 -0.3 269,980 + RR42_RS01175 0.78 +0.2 269,980 + RR42_RS01175 0.78 +0.0 269,980 + RR42_RS01175 0.78 +0.2 269,980 + RR42_RS01175 0.78 +1.0 269,981 - RR42_RS01175 0.79 +0.6 269,981 - RR42_RS01175 0.79 -0.3 269,981 - RR42_RS01175 0.79 +1.1 269,981 - RR42_RS01175 0.79 -0.5 270,034 + RR42_RS01175 0.84 +1.7 270,387 - RR42_RS01180 0.12 -0.2 270,737 + RR42_RS01180 0.55 +1.0 270,737 + RR42_RS01180 0.55 +0.1 270,737 + RR42_RS01180 0.55 +0.6 270,944 + RR42_RS01180 0.79 +1.2 270,944 + RR42_RS01180 0.79 +0.2 270,944 + RR42_RS01180 0.79 -0.3 270,944 + RR42_RS01180 0.79 -1.0 270,945 - RR42_RS01180 0.80 +1.0 271,103 + +0.4 271,103 + -0.3 271,103 + -0.1 271,103 + +1.3 271,104 - +0.4 271,104 - +1.5 271,104 - +0.2 271,104 - +1.1 271,104 - -0.4 271,112 + +0.4 271,112 + -0.0 271,112 + +1.6 271,113 - +0.2 271,113 - +1.3 271,113 - -0.3 271,171 - +0.2 271,171 - +0.6
Or see this region's nucleotide sequence