Experiment: Glycerol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_503 and Pf6N2E2_504 overlap by 23 nucleotides Pf6N2E2_504 and Pf6N2E2_505 are separated by 24 nucleotides Pf6N2E2_505 and Pf6N2E2_506 overlap by 35 nucleotides Pf6N2E2_506 and Pf6N2E2_507 overlap by 4 nucleotides
Pf6N2E2_503 - sensor histidine kinase, at 572,701 to 574,086
_503
Pf6N2E2_504 - DNA-binding response regulator, at 574,064 to 574,747
_504
Pf6N2E2_505 - hypothetical protein, at 574,772 to 575,053
_505
Pf6N2E2_506 - Hydrogen cyanide synthase HcnA / Opine oxidase subunit C, at 575,019 to 575,342
_506
Pf6N2E2_507 - Hydrogen cyanide synthase HcnB / Opine oxidase subunit A, at 575,339 to 576,748
_507
Position (kb)
574
575 Strain fitness (log2 ratio)
-2
-1
0
1 at 573.110 kb on + strand, within Pf6N2E2_503 at 573.110 kb on + strand, within Pf6N2E2_503 at 573.133 kb on + strand, within Pf6N2E2_503 at 573.652 kb on + strand, within Pf6N2E2_503 at 573.967 kb on + strand at 573.968 kb on - strand at 573.968 kb on - strand at 573.974 kb on + strand at 573.974 kb on + strand at 573.974 kb on + strand at 573.975 kb on - strand at 573.975 kb on - strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 574.025 kb on + strand at 574.026 kb on - strand at 574.026 kb on - strand at 574.026 kb on - strand at 574.197 kb on + strand, within Pf6N2E2_504 at 574.276 kb on - strand, within Pf6N2E2_504 at 574.361 kb on + strand, within Pf6N2E2_504 at 574.612 kb on - strand, within Pf6N2E2_504 at 574.776 kb on + strand at 574.776 kb on + strand at 574.802 kb on - strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.890 kb on + strand, within Pf6N2E2_505 at 574.932 kb on + strand, within Pf6N2E2_505 at 574.933 kb on - strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.038 kb on + strand at 575.038 kb on + strand at 575.039 kb on - strand at 575.202 kb on + strand, within Pf6N2E2_506 at 575.202 kb on + strand, within Pf6N2E2_506 at 575.218 kb on + strand, within Pf6N2E2_506 at 575.219 kb on - strand, within Pf6N2E2_506 at 575.219 kb on - strand, within Pf6N2E2_506 at 575.219 kb on - strand, within Pf6N2E2_506 at 575.219 kb on - strand, within Pf6N2E2_506 at 575.219 kb on - strand, within Pf6N2E2_506 at 575.498 kb on + strand, within Pf6N2E2_507
Per-strain Table
Position Strand Gene LocusTag Fraction Glycerol (C) remove 573,110 + Pf6N2E2_503 0.30 -0.5 573,110 + Pf6N2E2_503 0.30 -0.2 573,133 + Pf6N2E2_503 0.31 -0.0 573,652 + Pf6N2E2_503 0.69 -0.6 573,967 + +0.2 573,968 - +0.6 573,968 - -0.6 573,974 + -0.4 573,974 + +0.4 573,974 + +0.2 573,975 - -0.3 573,975 - -0.0 573,977 + -1.9 573,977 + -0.2 573,977 + +0.2 573,977 + -0.6 573,977 + +0.2 573,978 - +0.1 573,978 - +0.2 573,978 - -1.0 573,978 - -0.5 573,978 - -0.2 574,025 + -0.7 574,026 - -0.7 574,026 - -0.4 574,026 - -0.9 574,197 + Pf6N2E2_504 0.19 -0.2 574,276 - Pf6N2E2_504 0.31 -0.8 574,361 + Pf6N2E2_504 0.43 -1.2 574,612 - Pf6N2E2_504 0.80 -0.4 574,776 + -1.4 574,776 + -0.7 574,802 - Pf6N2E2_505 0.11 +0.5 574,878 + Pf6N2E2_505 0.38 -0.3 574,878 + Pf6N2E2_505 0.38 -0.4 574,878 + Pf6N2E2_505 0.38 -1.6 574,890 + Pf6N2E2_505 0.42 +0.2 574,932 + Pf6N2E2_505 0.57 -0.7 574,933 - Pf6N2E2_505 0.57 +0.4 575,015 + Pf6N2E2_505 0.86 +0.3 575,015 + Pf6N2E2_505 0.86 -2.3 575,015 + Pf6N2E2_505 0.86 +0.6 575,015 + Pf6N2E2_505 0.86 +0.3 575,016 - Pf6N2E2_505 0.87 +0.3 575,016 - Pf6N2E2_505 0.87 +1.3 575,016 - Pf6N2E2_505 0.87 +0.7 575,016 - Pf6N2E2_505 0.87 +1.5 575,038 + +0.3 575,038 + +0.2 575,039 - -0.1 575,202 + Pf6N2E2_506 0.56 +0.2 575,202 + Pf6N2E2_506 0.56 -0.1 575,218 + Pf6N2E2_506 0.61 -0.5 575,219 - Pf6N2E2_506 0.62 +0.5 575,219 - Pf6N2E2_506 0.62 +0.1 575,219 - Pf6N2E2_506 0.62 -0.2 575,219 - Pf6N2E2_506 0.62 +0.8 575,219 - Pf6N2E2_506 0.62 +1.0 575,498 + Pf6N2E2_507 0.11 +0.0
Or see this region's nucleotide sequence