Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5866 and Pf1N1B4_5867 are separated by 102 nucleotides Pf1N1B4_5867 and Pf1N1B4_5868 are separated by 105 nucleotides Pf1N1B4_5868 and Pf1N1B4_5869 overlap by 4 nucleotides Pf1N1B4_5869 and Pf1N1B4_5870 are separated by 17 nucleotides
Pf1N1B4_5866 - ThiJ/PfpI family protein, at 301,789 to 302,466
_5866
Pf1N1B4_5867 - hypothetical protein, at 302,569 to 302,763
_5867
Pf1N1B4_5868 - Secreted protein Hcp, at 302,869 to 303,384
_5868
Pf1N1B4_5869 - hypothetical protein, at 303,381 to 303,497
_5869
Pf1N1B4_5870 - VgrG protein, at 303,515 to 305,557
_5870
Position (kb) 302
303
304 Strain fitness (log2 ratio) -2
-1
0
1 at 301.878 kb on - strand, within Pf1N1B4_5866 at 302.186 kb on + strand, within Pf1N1B4_5866 at 302.227 kb on + strand, within Pf1N1B4_5866 at 302.228 kb on - strand, within Pf1N1B4_5866 at 302.372 kb on + strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.376 kb on - strand, within Pf1N1B4_5866 at 302.376 kb on - strand, within Pf1N1B4_5866 at 302.642 kb on - strand, within Pf1N1B4_5867 at 302.776 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.960 kb on - strand, within Pf1N1B4_5868 at 303.059 kb on + strand, within Pf1N1B4_5868 at 303.089 kb on - strand at 303.196 kb on + strand at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.200 kb on - strand, within Pf1N1B4_5868 at 303.338 kb on + strand at 303.339 kb on - strand at 303.383 kb on - strand at 303.810 kb on - strand, within Pf1N1B4_5870 at 303.851 kb on - strand, within Pf1N1B4_5870 at 304.268 kb on + strand, within Pf1N1B4_5870
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 301,878 - Pf1N1B4_5866 0.13 -1.2 302,186 + Pf1N1B4_5866 0.59 -0.5 302,227 + Pf1N1B4_5866 0.65 +1.1 302,228 - Pf1N1B4_5866 0.65 +0.8 302,372 + Pf1N1B4_5866 0.86 +0.2 302,373 - Pf1N1B4_5866 0.86 +0.8 302,373 - Pf1N1B4_5866 0.86 +0.5 302,373 - Pf1N1B4_5866 0.86 +0.5 302,373 - Pf1N1B4_5866 0.86 +0.6 302,376 - Pf1N1B4_5866 0.87 +0.3 302,376 - Pf1N1B4_5866 0.87 -1.5 302,642 - Pf1N1B4_5867 0.37 +1.5 302,776 + -1.5 302,884 + -1.5 302,884 + +0.5 302,884 + -0.8 302,884 + -0.5 302,884 + +1.2 302,884 + +1.5 302,884 + +0.2 302,884 + +0.8 302,885 - +0.1 302,885 - -2.1 302,885 - -0.7 302,885 - +1.2 302,885 - +0.0 302,885 - -0.5 302,885 - +0.1 302,885 - -0.1 302,885 - -0.9 302,960 - Pf1N1B4_5868 0.18 +0.6 303,059 + Pf1N1B4_5868 0.37 +1.0 303,089 - +0.8 303,196 + +0.2 303,197 - Pf1N1B4_5868 0.64 +1.0 303,197 - Pf1N1B4_5868 0.64 +0.8 303,197 - Pf1N1B4_5868 0.64 -0.0 303,199 + Pf1N1B4_5868 0.64 +0.0 303,199 + Pf1N1B4_5868 0.64 +0.5 303,199 + -0.5 303,199 + Pf1N1B4_5868 0.64 +0.6 303,199 + Pf1N1B4_5868 0.64 -1.7 303,200 - Pf1N1B4_5868 0.64 -0.2 303,338 + -0.5 303,339 - -1.2 303,383 - +0.6 303,810 - Pf1N1B4_5870 0.14 -0.6 303,851 - Pf1N1B4_5870 0.16 -0.0 304,268 + Pf1N1B4_5870 0.37 -1.3
Or see this region's nucleotide sequence