Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5554 and Pf1N1B4_5555 are separated by 172 nucleotides Pf1N1B4_5555 and Pf1N1B4_5556 are separated by 203 nucleotides Pf1N1B4_5556 and Pf1N1B4_5557 are separated by 200 nucleotides Pf1N1B4_5557 and Pf1N1B4_5558 are separated by 64 nucleotides
Pf1N1B4_5554 - hypothetical protein, at 326,976 to 327,200
_5554
Pf1N1B4_5555 - hypothetical protein, at 327,373 to 327,657
_5555
Pf1N1B4_5556 - hypothetical protein, at 327,861 to 328,058
_5556
Pf1N1B4_5557 - hypothetical protein, at 328,259 to 328,720
_5557
Pf1N1B4_5558 - hypothetical protein, at 328,785 to 329,069
_5558
Position (kb) 327
328
329 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 326.992 kb on + strand at 327.041 kb on - strand, within Pf1N1B4_5554 at 327.041 kb on - strand, within Pf1N1B4_5554 at 327.098 kb on - strand, within Pf1N1B4_5554 at 327.098 kb on - strand, within Pf1N1B4_5554 at 327.098 kb on - strand, within Pf1N1B4_5554 at 327.193 kb on - strand at 327.219 kb on - strand at 327.219 kb on - strand at 327.219 kb on - strand at 327.294 kb on - strand at 327.294 kb on - strand at 327.294 kb on - strand at 327.361 kb on - strand at 327.392 kb on + strand at 327.393 kb on - strand at 327.396 kb on - strand at 327.396 kb on - strand at 327.396 kb on - strand at 327.467 kb on + strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.468 kb on - strand, within Pf1N1B4_5555 at 327.473 kb on + strand, within Pf1N1B4_5555 at 327.474 kb on - strand, within Pf1N1B4_5555 at 327.474 kb on - strand at 327.530 kb on - strand, within Pf1N1B4_5555 at 327.580 kb on + strand, within Pf1N1B4_5555 at 327.581 kb on - strand, within Pf1N1B4_5555 at 327.581 kb on - strand, within Pf1N1B4_5555 at 327.581 kb on - strand, within Pf1N1B4_5555 at 327.601 kb on - strand, within Pf1N1B4_5555 at 327.601 kb on - strand, within Pf1N1B4_5555 at 327.608 kb on + strand, within Pf1N1B4_5555 at 327.609 kb on - strand, within Pf1N1B4_5555 at 327.805 kb on + strand at 327.806 kb on - strand at 327.888 kb on - strand, within Pf1N1B4_5556 at 327.952 kb on + strand, within Pf1N1B4_5556 at 327.953 kb on - strand, within Pf1N1B4_5556 at 327.953 kb on - strand at 327.953 kb on - strand, within Pf1N1B4_5556 at 327.993 kb on - strand, within Pf1N1B4_5556 at 328.144 kb on - strand at 328.193 kb on + strand at 328.193 kb on + strand at 328.193 kb on + strand at 328.193 kb on + strand at 328.194 kb on - strand at 328.194 kb on - strand at 328.194 kb on - strand at 328.225 kb on + strand at 328.225 kb on + strand at 328.225 kb on + strand at 328.225 kb on + strand at 328.225 kb on + strand at 328.225 kb on + strand at 328.226 kb on - strand at 328.244 kb on - strand at 328.261 kb on - strand at 328.261 kb on - strand at 328.352 kb on + strand, within Pf1N1B4_5557 at 328.352 kb on + strand, within Pf1N1B4_5557 at 328.378 kb on + strand, within Pf1N1B4_5557 at 328.390 kb on + strand, within Pf1N1B4_5557 at 328.391 kb on - strand, within Pf1N1B4_5557 at 328.391 kb on - strand, within Pf1N1B4_5557 at 328.391 kb on - strand, within Pf1N1B4_5557 at 328.392 kb on + strand, within Pf1N1B4_5557 at 328.392 kb on + strand, within Pf1N1B4_5557 at 328.392 kb on + strand, within Pf1N1B4_5557 at 328.392 kb on + strand, within Pf1N1B4_5557 at 328.392 kb on + strand, within Pf1N1B4_5557 at 328.392 kb on + strand at 328.392 kb on + strand, within Pf1N1B4_5557 at 328.392 kb on + strand, within Pf1N1B4_5557 at 328.392 kb on + strand, within Pf1N1B4_5557 at 328.392 kb on + strand at 328.392 kb on + strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.393 kb on - strand, within Pf1N1B4_5557 at 328.408 kb on + strand at 328.408 kb on + strand, within Pf1N1B4_5557 at 328.409 kb on - strand, within Pf1N1B4_5557 at 328.670 kb on + strand, within Pf1N1B4_5557 at 328.673 kb on + strand, within Pf1N1B4_5557 at 328.674 kb on - strand at 328.674 kb on - strand at 328.674 kb on - strand at 328.674 kb on - strand at 328.759 kb on + strand at 328.759 kb on + strand at 328.759 kb on + strand at 328.759 kb on + strand at 328.760 kb on - strand at 328.760 kb on - strand at 328.760 kb on - strand at 328.760 kb on - strand at 328.760 kb on - strand at 328.760 kb on - strand at 328.760 kb on - strand at 328.760 kb on - strand at 328.802 kb on + strand at 328.803 kb on - strand at 328.803 kb on - strand at 328.803 kb on - strand at 328.803 kb on - strand at 328.806 kb on + strand at 328.806 kb on + strand at 328.807 kb on - strand at 328.879 kb on + strand, within Pf1N1B4_5558 at 328.880 kb on - strand, within Pf1N1B4_5558 at 328.880 kb on - strand, within Pf1N1B4_5558 at 328.890 kb on + strand, within Pf1N1B4_5558 at 328.890 kb on + strand, within Pf1N1B4_5558 at 328.890 kb on + strand, within Pf1N1B4_5558 at 328.890 kb on + strand, within Pf1N1B4_5558 at 328.891 kb on - strand, within Pf1N1B4_5558 at 328.891 kb on - strand, within Pf1N1B4_5558 at 328.913 kb on + strand, within Pf1N1B4_5558 at 328.913 kb on + strand, within Pf1N1B4_5558 at 328.913 kb on + strand, within Pf1N1B4_5558 at 328.914 kb on - strand, within Pf1N1B4_5558 at 328.995 kb on + strand, within Pf1N1B4_5558 at 328.995 kb on + strand, within Pf1N1B4_5558 at 328.996 kb on - strand, within Pf1N1B4_5558 at 328.996 kb on - strand at 329.038 kb on - strand, within Pf1N1B4_5558
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 326,992 + +0.2 327,041 - Pf1N1B4_5554 0.29 +0.2 327,041 - Pf1N1B4_5554 0.29 +0.3 327,098 - Pf1N1B4_5554 0.54 +0.7 327,098 - Pf1N1B4_5554 0.54 +0.0 327,098 - Pf1N1B4_5554 0.54 +0.2 327,193 - +1.4 327,219 - -0.6 327,219 - -0.6 327,219 - -0.4 327,294 - +0.7 327,294 - +0.9 327,294 - +0.3 327,361 - +1.0 327,392 + +0.6 327,393 - +1.3 327,396 - -0.9 327,396 - +0.7 327,396 - +1.0 327,467 + Pf1N1B4_5555 0.33 +1.3 327,468 - Pf1N1B4_5555 0.33 +0.7 327,468 - Pf1N1B4_5555 0.33 +0.5 327,468 - Pf1N1B4_5555 0.33 -1.3 327,468 - Pf1N1B4_5555 0.33 +1.3 327,468 - Pf1N1B4_5555 0.33 +2.0 327,468 - Pf1N1B4_5555 0.33 +0.4 327,473 + Pf1N1B4_5555 0.35 -1.0 327,474 - Pf1N1B4_5555 0.35 +0.2 327,474 - +1.4 327,530 - Pf1N1B4_5555 0.55 -0.3 327,580 + Pf1N1B4_5555 0.73 -1.0 327,581 - Pf1N1B4_5555 0.73 -0.6 327,581 - Pf1N1B4_5555 0.73 -0.4 327,581 - Pf1N1B4_5555 0.73 -0.8 327,601 - Pf1N1B4_5555 0.80 +0.9 327,601 - Pf1N1B4_5555 0.80 +0.6 327,608 + Pf1N1B4_5555 0.82 +1.2 327,609 - Pf1N1B4_5555 0.83 +0.4 327,805 + -1.4 327,806 - -0.6 327,888 - Pf1N1B4_5556 0.14 +0.1 327,952 + Pf1N1B4_5556 0.46 +3.6 327,953 - Pf1N1B4_5556 0.46 +0.7 327,953 - -0.8 327,953 - Pf1N1B4_5556 0.46 +0.0 327,993 - Pf1N1B4_5556 0.67 +0.8 328,144 - -0.8 328,193 + -1.3 328,193 + -0.3 328,193 + -1.0 328,193 + +0.0 328,194 - -1.0 328,194 - +0.4 328,194 - -0.6 328,225 + -1.0 328,225 + -0.2 328,225 + +0.4 328,225 + +0.4 328,225 + -0.3 328,225 + -0.5 328,226 - +0.7 328,244 - -1.1 328,261 - +0.4 328,261 - +1.0 328,352 + Pf1N1B4_5557 0.20 -1.0 328,352 + Pf1N1B4_5557 0.20 +0.2 328,378 + Pf1N1B4_5557 0.26 +0.8 328,390 + Pf1N1B4_5557 0.28 +0.7 328,391 - Pf1N1B4_5557 0.29 -0.6 328,391 - Pf1N1B4_5557 0.29 +0.8 328,391 - Pf1N1B4_5557 0.29 -0.9 328,392 + Pf1N1B4_5557 0.29 +1.0 328,392 + Pf1N1B4_5557 0.29 +1.0 328,392 + Pf1N1B4_5557 0.29 -0.1 328,392 + Pf1N1B4_5557 0.29 +0.4 328,392 + Pf1N1B4_5557 0.29 -1.2 328,392 + +2.0 328,392 + Pf1N1B4_5557 0.29 -0.4 328,392 + Pf1N1B4_5557 0.29 +1.5 328,392 + Pf1N1B4_5557 0.29 -0.7 328,392 + -0.3 328,392 + Pf1N1B4_5557 0.29 +0.2 328,393 - Pf1N1B4_5557 0.29 -1.1 328,393 - Pf1N1B4_5557 0.29 -0.6 328,393 - Pf1N1B4_5557 0.29 +1.0 328,393 - Pf1N1B4_5557 0.29 +0.7 328,393 - Pf1N1B4_5557 0.29 +0.2 328,393 - Pf1N1B4_5557 0.29 +1.0 328,393 - Pf1N1B4_5557 0.29 -1.7 328,393 - Pf1N1B4_5557 0.29 +0.7 328,393 - Pf1N1B4_5557 0.29 -0.1 328,393 - Pf1N1B4_5557 0.29 +0.8 328,393 - Pf1N1B4_5557 0.29 -0.6 328,393 - Pf1N1B4_5557 0.29 -0.1 328,393 - Pf1N1B4_5557 0.29 +0.7 328,408 + +0.8 328,408 + Pf1N1B4_5557 0.32 +2.4 328,409 - Pf1N1B4_5557 0.32 -1.8 328,670 + Pf1N1B4_5557 0.89 -1.1 328,673 + Pf1N1B4_5557 0.90 +0.0 328,674 - -0.8 328,674 - -0.9 328,674 - +0.1 328,674 - +1.4 328,759 + -0.6 328,759 + +0.3 328,759 + +0.2 328,759 + -0.6 328,760 - -1.2 328,760 - +0.4 328,760 - +0.2 328,760 - +0.4 328,760 - +1.4 328,760 - +0.7 328,760 - +2.0 328,760 - +1.6 328,802 + +0.4 328,803 - -0.4 328,803 - +0.4 328,803 - +0.6 328,803 - +3.3 328,806 + +0.2 328,806 + +0.4 328,807 - +0.1 328,879 + Pf1N1B4_5558 0.33 +1.3 328,880 - Pf1N1B4_5558 0.33 +0.4 328,880 - Pf1N1B4_5558 0.33 +0.9 328,890 + Pf1N1B4_5558 0.37 -0.4 328,890 + Pf1N1B4_5558 0.37 -0.3 328,890 + Pf1N1B4_5558 0.37 +0.0 328,890 + Pf1N1B4_5558 0.37 -0.4 328,891 - Pf1N1B4_5558 0.37 -1.0 328,891 - Pf1N1B4_5558 0.37 -0.8 328,913 + Pf1N1B4_5558 0.45 -0.3 328,913 + Pf1N1B4_5558 0.45 +0.8 328,913 + Pf1N1B4_5558 0.45 -0.2 328,914 - Pf1N1B4_5558 0.45 +0.7 328,995 + Pf1N1B4_5558 0.74 +1.4 328,995 + Pf1N1B4_5558 0.74 +0.7 328,996 - Pf1N1B4_5558 0.74 -1.7 328,996 - -0.6 329,038 - Pf1N1B4_5558 0.89 -1.0
Or see this region's nucleotide sequence