Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2474 and Pf1N1B4_2475 are separated by 291 nucleotides Pf1N1B4_2475 and Pf1N1B4_2476 overlap by 4 nucleotides Pf1N1B4_2476 and Pf1N1B4_2477 are separated by 266 nucleotides Pf1N1B4_2477 and Pf1N1B4_2478 are separated by 173 nucleotides
Pf1N1B4_2474 - RNA polymerase sigma-70 factor, ECF subfamily, at 2,806,082 to 2,806,558
_2474
Pf1N1B4_2475 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 2,806,850 to 2,807,557
_2475
Pf1N1B4_2476 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 2,807,554 to 2,809,494
_2476
Pf1N1B4_2477 - response regulator, at 2,809,761 to 2,810,213
_2477
Pf1N1B4_2478 - Malate synthase G (EC 2.3.3.9), at 2,810,387 to 2,812,564
_2478
Position (kb)
2807
2808
2809
2810 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2806.554 kb on + strand at 2806.669 kb on + strand at 2806.669 kb on + strand at 2806.670 kb on - strand at 2806.851 kb on + strand at 2806.851 kb on + strand at 2806.851 kb on + strand at 2806.915 kb on + strand at 2806.916 kb on - strand at 2807.121 kb on + strand, within Pf1N1B4_2475 at 2807.122 kb on - strand, within Pf1N1B4_2475 at 2807.395 kb on + strand, within Pf1N1B4_2475 at 2807.429 kb on + strand, within Pf1N1B4_2475 at 2807.557 kb on + strand at 2807.557 kb on + strand at 2807.557 kb on + strand at 2807.558 kb on - strand at 2807.558 kb on - strand at 2807.558 kb on - strand at 2807.692 kb on + strand at 2807.693 kb on - strand at 2807.693 kb on - strand at 2807.732 kb on + strand at 2808.064 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.567 kb on + strand, within Pf1N1B4_2476 at 2808.568 kb on - strand, within Pf1N1B4_2476 at 2808.568 kb on - strand, within Pf1N1B4_2476 at 2808.568 kb on - strand, within Pf1N1B4_2476 at 2808.568 kb on - strand, within Pf1N1B4_2476 at 2808.728 kb on + strand, within Pf1N1B4_2476 at 2808.729 kb on - strand, within Pf1N1B4_2476 at 2808.802 kb on + strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.803 kb on - strand, within Pf1N1B4_2476 at 2808.901 kb on + strand, within Pf1N1B4_2476 at 2808.902 kb on - strand, within Pf1N1B4_2476 at 2809.104 kb on + strand, within Pf1N1B4_2476 at 2809.104 kb on + strand, within Pf1N1B4_2476 at 2809.114 kb on + strand at 2809.125 kb on + strand, within Pf1N1B4_2476 at 2809.125 kb on + strand, within Pf1N1B4_2476 at 2809.126 kb on - strand, within Pf1N1B4_2476 at 2809.280 kb on + strand, within Pf1N1B4_2476 at 2809.280 kb on + strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.281 kb on - strand, within Pf1N1B4_2476 at 2809.334 kb on + strand at 2809.335 kb on - strand at 2809.335 kb on - strand at 2809.335 kb on - strand at 2809.364 kb on + strand at 2809.411 kb on + strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.412 kb on - strand at 2809.489 kb on - strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.587 kb on + strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.588 kb on - strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.593 kb on + strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.594 kb on - strand at 2809.597 kb on + strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.598 kb on - strand at 2809.619 kb on - strand at 2809.656 kb on + strand at 2809.656 kb on + strand at 2809.657 kb on - strand at 2809.657 kb on - strand at 2809.775 kb on + strand at 2809.775 kb on + strand at 2809.776 kb on - strand at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2809.954 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.128 kb on + strand, within Pf1N1B4_2477 at 2810.129 kb on - strand, within Pf1N1B4_2477 at 2810.385 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 2,806,554 + +0.1 2,806,669 + +0.5 2,806,669 + +0.9 2,806,670 - +0.8 2,806,851 + +0.5 2,806,851 + -0.8 2,806,851 + +0.4 2,806,915 + -0.1 2,806,916 - +1.5 2,807,121 + Pf1N1B4_2475 0.38 -0.4 2,807,122 - Pf1N1B4_2475 0.38 +0.3 2,807,395 + Pf1N1B4_2475 0.77 +0.2 2,807,429 + Pf1N1B4_2475 0.82 -0.5 2,807,557 + +0.2 2,807,557 + -0.7 2,807,557 + +0.7 2,807,558 - -1.5 2,807,558 - -0.2 2,807,558 - +0.1 2,807,692 + +2.5 2,807,693 - +0.8 2,807,693 - +0.6 2,807,732 + -0.8 2,808,064 + Pf1N1B4_2476 0.26 +1.0 2,808,080 + Pf1N1B4_2476 0.27 +0.5 2,808,080 + Pf1N1B4_2476 0.27 -0.3 2,808,080 + Pf1N1B4_2476 0.27 +0.8 2,808,080 + Pf1N1B4_2476 0.27 -0.1 2,808,081 - Pf1N1B4_2476 0.27 +1.5 2,808,081 - Pf1N1B4_2476 0.27 -1.5 2,808,081 - Pf1N1B4_2476 0.27 +1.0 2,808,081 - +0.5 2,808,081 - Pf1N1B4_2476 0.27 +0.2 2,808,081 - Pf1N1B4_2476 0.27 -0.3 2,808,081 - Pf1N1B4_2476 0.27 +1.8 2,808,567 + Pf1N1B4_2476 0.52 +0.4 2,808,568 - Pf1N1B4_2476 0.52 +0.6 2,808,568 - Pf1N1B4_2476 0.52 -0.9 2,808,568 - Pf1N1B4_2476 0.52 +0.5 2,808,568 - Pf1N1B4_2476 0.52 +0.5 2,808,728 + Pf1N1B4_2476 0.60 +0.5 2,808,729 - Pf1N1B4_2476 0.61 +0.1 2,808,802 + Pf1N1B4_2476 0.64 +0.5 2,808,803 - Pf1N1B4_2476 0.64 +0.5 2,808,803 - Pf1N1B4_2476 0.64 -0.5 2,808,803 - Pf1N1B4_2476 0.64 +1.5 2,808,803 - Pf1N1B4_2476 0.64 -0.4 2,808,803 - Pf1N1B4_2476 0.64 +0.8 2,808,901 + Pf1N1B4_2476 0.69 +0.9 2,808,902 - Pf1N1B4_2476 0.69 +0.6 2,809,104 + Pf1N1B4_2476 0.80 +1.4 2,809,104 + Pf1N1B4_2476 0.80 +0.9 2,809,114 + +2.5 2,809,125 + Pf1N1B4_2476 0.81 -0.1 2,809,125 + Pf1N1B4_2476 0.81 +0.2 2,809,126 - Pf1N1B4_2476 0.81 +0.9 2,809,280 + Pf1N1B4_2476 0.89 +1.5 2,809,280 + Pf1N1B4_2476 0.89 +0.8 2,809,281 - Pf1N1B4_2476 0.89 +0.9 2,809,281 - Pf1N1B4_2476 0.89 +0.7 2,809,281 - Pf1N1B4_2476 0.89 +0.3 2,809,334 + -0.4 2,809,335 - -0.8 2,809,335 - +0.8 2,809,335 - -0.8 2,809,364 + -0.3 2,809,411 + +0.0 2,809,412 - -0.5 2,809,412 - +2.1 2,809,412 - +0.5 2,809,489 - -0.5 2,809,587 + -1.3 2,809,587 + +0.5 2,809,587 + -0.6 2,809,587 + +0.5 2,809,587 + -3.0 2,809,587 + +0.9 2,809,587 + -0.6 2,809,587 + +0.5 2,809,588 - -0.5 2,809,588 - -0.8 2,809,588 - -2.2 2,809,588 - -0.3 2,809,588 - -0.2 2,809,593 + +0.9 2,809,593 + +0.5 2,809,593 + +1.9 2,809,594 - -0.9 2,809,594 - -0.3 2,809,594 - -0.1 2,809,594 - -0.3 2,809,594 - -0.2 2,809,594 - -2.1 2,809,594 - -1.1 2,809,594 - -1.0 2,809,594 - -0.7 2,809,597 + -0.3 2,809,598 - -0.4 2,809,598 - -0.3 2,809,598 - +0.9 2,809,619 - -1.6 2,809,656 + -1.1 2,809,656 + +1.5 2,809,657 - +0.1 2,809,657 - +0.3 2,809,775 + -0.4 2,809,775 + -0.5 2,809,776 - -0.8 2,809,954 + Pf1N1B4_2477 0.43 -0.8 2,809,954 + Pf1N1B4_2477 0.43 -0.2 2,809,954 + Pf1N1B4_2477 0.43 -1.1 2,809,954 + Pf1N1B4_2477 0.43 +1.5 2,810,128 + Pf1N1B4_2477 0.81 -1.5 2,810,128 + Pf1N1B4_2477 0.81 -2.2 2,810,128 + Pf1N1B4_2477 0.81 -0.1 2,810,129 - Pf1N1B4_2477 0.81 -0.2 2,810,385 - -1.7
Or see this region's nucleotide sequence