Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2363 and Pf1N1B4_2364 overlap by 8 nucleotides Pf1N1B4_2364 and Pf1N1B4_2365 overlap by 4 nucleotides Pf1N1B4_2365 and Pf1N1B4_2366 overlap by 4 nucleotides Pf1N1B4_2366 and Pf1N1B4_2367 overlap by 4 nucleotides
Pf1N1B4_2363 - Malonate decarboxylase delta subunit, at 2,681,086 to 2,681,385
_2363
Pf1N1B4_2364 - Malonate decarboxylase beta subunit, at 2,681,378 to 2,682,229
_2364
Pf1N1B4_2365 - Malonate decarboxylase gamma subunit, at 2,682,226 to 2,683,029
_2365
Pf1N1B4_2366 - Phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.-), at 2,683,026 to 2,683,643
_2366
Pf1N1B4_2367 - Malonyl CoA acyl carrier protein transacylase (EC 2.3.1.39), at 2,683,640 to 2,684,551
_2367
Position (kb)
2682
2683
2684 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2681.410 kb on + strand at 2681.410 kb on + strand at 2681.410 kb on + strand at 2681.410 kb on + strand at 2681.410 kb on + strand at 2681.411 kb on - strand at 2681.411 kb on - strand at 2681.411 kb on - strand at 2681.411 kb on - strand at 2681.411 kb on - strand at 2681.411 kb on - strand at 2681.411 kb on - strand at 2681.411 kb on - strand at 2681.891 kb on + strand, within Pf1N1B4_2364 at 2681.891 kb on + strand, within Pf1N1B4_2364 at 2681.891 kb on + strand, within Pf1N1B4_2364 at 2681.892 kb on - strand, within Pf1N1B4_2364 at 2681.892 kb on - strand, within Pf1N1B4_2364 at 2681.969 kb on + strand, within Pf1N1B4_2364 at 2681.969 kb on + strand, within Pf1N1B4_2364 at 2681.970 kb on - strand, within Pf1N1B4_2364 at 2681.970 kb on - strand, within Pf1N1B4_2364 at 2682.047 kb on + strand, within Pf1N1B4_2364 at 2682.047 kb on + strand, within Pf1N1B4_2364 at 2682.235 kb on + strand at 2682.235 kb on + strand at 2682.235 kb on + strand at 2682.329 kb on + strand, within Pf1N1B4_2365 at 2682.355 kb on - strand, within Pf1N1B4_2365 at 2682.526 kb on + strand, within Pf1N1B4_2365 at 2682.527 kb on - strand, within Pf1N1B4_2365 at 2682.586 kb on + strand, within Pf1N1B4_2365 at 2682.586 kb on + strand, within Pf1N1B4_2365 at 2682.586 kb on + strand, within Pf1N1B4_2365 at 2682.586 kb on + strand, within Pf1N1B4_2365 at 2682.586 kb on + strand, within Pf1N1B4_2365 at 2682.587 kb on - strand, within Pf1N1B4_2365 at 2682.587 kb on - strand, within Pf1N1B4_2365 at 2682.587 kb on - strand, within Pf1N1B4_2365 at 2682.587 kb on - strand, within Pf1N1B4_2365 at 2682.808 kb on + strand, within Pf1N1B4_2365 at 2682.808 kb on + strand, within Pf1N1B4_2365 at 2682.808 kb on + strand, within Pf1N1B4_2365 at 2682.809 kb on - strand, within Pf1N1B4_2365 at 2683.246 kb on + strand, within Pf1N1B4_2366 at 2683.246 kb on + strand, within Pf1N1B4_2366 at 2683.527 kb on + strand, within Pf1N1B4_2366 at 2683.537 kb on + strand, within Pf1N1B4_2366 at 2683.537 kb on + strand, within Pf1N1B4_2366 at 2683.537 kb on + strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.538 kb on - strand, within Pf1N1B4_2366 at 2683.800 kb on + strand, within Pf1N1B4_2367 at 2683.800 kb on + strand, within Pf1N1B4_2367 at 2683.800 kb on + strand, within Pf1N1B4_2367 at 2683.800 kb on + strand at 2683.801 kb on - strand, within Pf1N1B4_2367 at 2683.856 kb on + strand, within Pf1N1B4_2367 at 2683.857 kb on - strand, within Pf1N1B4_2367 at 2683.982 kb on + strand, within Pf1N1B4_2367 at 2683.982 kb on + strand, within Pf1N1B4_2367 at 2683.983 kb on - strand, within Pf1N1B4_2367
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 2,681,410 + -0.0 2,681,410 + +0.4 2,681,410 + +1.5 2,681,410 + -1.5 2,681,410 + +0.4 2,681,411 - +1.3 2,681,411 - -0.5 2,681,411 - -1.3 2,681,411 - +0.3 2,681,411 - -0.7 2,681,411 - +0.2 2,681,411 - +0.3 2,681,411 - +1.1 2,681,891 + Pf1N1B4_2364 0.60 -0.0 2,681,891 + Pf1N1B4_2364 0.60 +2.1 2,681,891 + Pf1N1B4_2364 0.60 +0.3 2,681,892 - Pf1N1B4_2364 0.60 +1.0 2,681,892 - Pf1N1B4_2364 0.60 -1.0 2,681,969 + Pf1N1B4_2364 0.69 -0.2 2,681,969 + Pf1N1B4_2364 0.69 +1.4 2,681,970 - Pf1N1B4_2364 0.69 -0.5 2,681,970 - Pf1N1B4_2364 0.69 +0.9 2,682,047 + Pf1N1B4_2364 0.79 +0.2 2,682,047 + Pf1N1B4_2364 0.79 -0.0 2,682,235 + -0.5 2,682,235 + -0.6 2,682,235 + -0.3 2,682,329 + Pf1N1B4_2365 0.13 -0.0 2,682,355 - Pf1N1B4_2365 0.16 +0.7 2,682,526 + Pf1N1B4_2365 0.37 +1.5 2,682,527 - Pf1N1B4_2365 0.37 +1.5 2,682,586 + Pf1N1B4_2365 0.45 +0.0 2,682,586 + Pf1N1B4_2365 0.45 -0.6 2,682,586 + Pf1N1B4_2365 0.45 +0.5 2,682,586 + Pf1N1B4_2365 0.45 +1.1 2,682,586 + Pf1N1B4_2365 0.45 -0.1 2,682,587 - Pf1N1B4_2365 0.45 -0.5 2,682,587 - Pf1N1B4_2365 0.45 +0.5 2,682,587 - Pf1N1B4_2365 0.45 +0.8 2,682,587 - Pf1N1B4_2365 0.45 -0.5 2,682,808 + Pf1N1B4_2365 0.72 -0.1 2,682,808 + Pf1N1B4_2365 0.72 +1.2 2,682,808 + Pf1N1B4_2365 0.72 +0.9 2,682,809 - Pf1N1B4_2365 0.73 -1.4 2,683,246 + Pf1N1B4_2366 0.36 +0.2 2,683,246 + Pf1N1B4_2366 0.36 -0.9 2,683,527 + Pf1N1B4_2366 0.81 +0.8 2,683,537 + Pf1N1B4_2366 0.83 +0.5 2,683,537 + Pf1N1B4_2366 0.83 +1.9 2,683,537 + Pf1N1B4_2366 0.83 +0.6 2,683,538 - Pf1N1B4_2366 0.83 -0.5 2,683,538 - Pf1N1B4_2366 0.83 +1.0 2,683,538 - Pf1N1B4_2366 0.83 -0.7 2,683,538 - Pf1N1B4_2366 0.83 -1.3 2,683,800 + Pf1N1B4_2367 0.18 +0.2 2,683,800 + Pf1N1B4_2367 0.18 -1.9 2,683,800 + Pf1N1B4_2367 0.18 +0.1 2,683,800 + +2.5 2,683,801 - Pf1N1B4_2367 0.18 +2.5 2,683,856 + Pf1N1B4_2367 0.24 -0.9 2,683,857 - Pf1N1B4_2367 0.24 +0.5 2,683,982 + Pf1N1B4_2367 0.38 +2.0 2,683,982 + Pf1N1B4_2367 0.38 -1.3 2,683,983 - Pf1N1B4_2367 0.38 -0.0
Or see this region's nucleotide sequence