Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_176 and Pf1N1B4_177 are separated by 151 nucleotides Pf1N1B4_177 and Pf1N1B4_178 are separated by 39 nucleotides Pf1N1B4_178 and Pf1N1B4_179 overlap by 8 nucleotides
Pf1N1B4_176 - Acetylpolyamine aminohydrolase, at 219,324 to 220,349
_176
Pf1N1B4_177 - N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87), at 220,501 to 221,730
_177
Pf1N1B4_178 - Demethylmenaquinone methyltransferase, at 221,770 to 222,381
_178
Pf1N1B4_179 - Endoribonuclease L-PSP, at 222,374 to 222,766
_179
Position (kb)
220
221
222 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 219.507 kb on - strand, within Pf1N1B4_176 at 219.718 kb on + strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.847 kb on + strand, within Pf1N1B4_176 at 219.847 kb on + strand, within Pf1N1B4_176 at 219.848 kb on - strand, within Pf1N1B4_176 at 219.908 kb on - strand, within Pf1N1B4_176 at 220.021 kb on + strand, within Pf1N1B4_176 at 220.022 kb on - strand, within Pf1N1B4_176 at 220.153 kb on + strand, within Pf1N1B4_176 at 220.277 kb on + strand at 220.298 kb on - strand at 220.298 kb on - strand at 220.298 kb on - strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.301 kb on - strand at 220.301 kb on - strand at 220.301 kb on - strand at 220.358 kb on + strand at 220.358 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.375 kb on - strand at 220.503 kb on - strand at 220.782 kb on + strand, within Pf1N1B4_177 at 220.783 kb on - strand, within Pf1N1B4_177 at 220.783 kb on - strand, within Pf1N1B4_177 at 220.929 kb on + strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 221.053 kb on - strand at 221.053 kb on - strand, within Pf1N1B4_177 at 221.053 kb on - strand, within Pf1N1B4_177 at 221.183 kb on + strand, within Pf1N1B4_177 at 221.184 kb on - strand at 221.184 kb on - strand at 221.447 kb on + strand at 221.718 kb on + strand at 221.719 kb on - strand at 221.748 kb on + strand at 222.495 kb on + strand, within Pf1N1B4_179 at 222.718 kb on - strand, within Pf1N1B4_179
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 219,507 - Pf1N1B4_176 0.18 +0.3 219,718 + Pf1N1B4_176 0.38 -2.1 219,719 - Pf1N1B4_176 0.38 -1.1 219,719 - Pf1N1B4_176 0.38 -0.1 219,719 - Pf1N1B4_176 0.38 +1.9 219,847 + Pf1N1B4_176 0.51 +1.7 219,847 + Pf1N1B4_176 0.51 +0.9 219,848 - Pf1N1B4_176 0.51 +1.3 219,908 - Pf1N1B4_176 0.57 -0.6 220,021 + Pf1N1B4_176 0.68 +0.9 220,022 - Pf1N1B4_176 0.68 -1.7 220,153 + Pf1N1B4_176 0.81 +0.3 220,277 + +0.5 220,298 - +0.7 220,298 - -0.3 220,298 - -0.1 220,300 + -0.7 220,300 + -0.2 220,300 + -0.4 220,300 + -1.7 220,300 + -0.5 220,300 + +0.3 220,301 - -0.4 220,301 - +1.4 220,301 - -0.7 220,358 + -0.9 220,358 + -0.1 220,374 + -0.5 220,374 + -0.4 220,374 + -1.7 220,374 + -0.3 220,374 + +1.9 220,375 - +0.7 220,503 - +1.1 220,782 + Pf1N1B4_177 0.23 +0.2 220,783 - Pf1N1B4_177 0.23 +0.5 220,783 - Pf1N1B4_177 0.23 +0.9 220,929 + Pf1N1B4_177 0.35 +0.1 220,930 - Pf1N1B4_177 0.35 +0.6 220,930 - Pf1N1B4_177 0.35 -0.1 220,930 - Pf1N1B4_177 0.35 +0.3 220,930 - Pf1N1B4_177 0.35 -0.3 221,053 - -0.1 221,053 - Pf1N1B4_177 0.45 -0.4 221,053 - Pf1N1B4_177 0.45 +0.7 221,183 + Pf1N1B4_177 0.55 +0.4 221,184 - -1.1 221,184 - +0.3 221,447 + -1.7 221,718 + -1.4 221,719 - +0.3 221,748 + -1.4 222,495 + Pf1N1B4_179 0.31 +0.7 222,718 - Pf1N1B4_179 0.88 +0.5
Or see this region's nucleotide sequence