Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1741 and Pf1N1B4_1742 are separated by 178 nucleotides Pf1N1B4_1742 and Pf1N1B4_1743 overlap by 4 nucleotides
Pf1N1B4_1741 - Ferredoxin reductase, at 1,989,831 to 1,991,360
_1741
Pf1N1B4_1742 - Biofilm PGA synthesis auxiliary protein PgaD, at 1,991,539 to 1,992,066
_1742
Pf1N1B4_1743 - Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-), at 1,992,063 to 1,993,418
_1743
Position (kb)
1991
1992
1993 Strain fitness (log2 ratio)
-2
-1
0
1 at 1990.543 kb on + strand, within Pf1N1B4_1741 at 1990.544 kb on - strand, within Pf1N1B4_1741 at 1991.104 kb on - strand, within Pf1N1B4_1741 at 1991.118 kb on + strand, within Pf1N1B4_1741 at 1991.149 kb on - strand, within Pf1N1B4_1741 at 1991.154 kb on + strand, within Pf1N1B4_1741 at 1991.154 kb on + strand, within Pf1N1B4_1741 at 1991.154 kb on + strand, within Pf1N1B4_1741 at 1991.387 kb on - strand at 1991.470 kb on + strand at 1991.470 kb on + strand at 1991.470 kb on + strand at 1991.470 kb on + strand at 1991.471 kb on - strand at 1991.471 kb on - strand at 1991.471 kb on - strand at 1991.471 kb on - strand at 1991.473 kb on - strand at 1991.491 kb on + strand at 1991.491 kb on + strand at 1991.540 kb on + strand at 1991.540 kb on + strand at 1991.541 kb on - strand at 1991.541 kb on - strand at 1991.629 kb on + strand, within Pf1N1B4_1742 at 1991.629 kb on + strand at 1991.694 kb on + strand, within Pf1N1B4_1742 at 1991.886 kb on - strand at 1991.886 kb on - strand, within Pf1N1B4_1742 at 1991.886 kb on - strand, within Pf1N1B4_1742 at 1991.977 kb on + strand, within Pf1N1B4_1742 at 1991.978 kb on - strand, within Pf1N1B4_1742 at 1991.978 kb on - strand, within Pf1N1B4_1742 at 1991.978 kb on - strand, within Pf1N1B4_1742 at 1991.978 kb on - strand, within Pf1N1B4_1742 at 1991.978 kb on - strand, within Pf1N1B4_1742 at 1991.981 kb on + strand, within Pf1N1B4_1742 at 1991.981 kb on + strand, within Pf1N1B4_1742 at 1991.982 kb on - strand, within Pf1N1B4_1742 at 1991.982 kb on - strand, within Pf1N1B4_1742 at 1991.982 kb on - strand, within Pf1N1B4_1742 at 1991.982 kb on - strand, within Pf1N1B4_1742 at 1991.982 kb on - strand, within Pf1N1B4_1742 at 1992.183 kb on - strand at 1992.183 kb on - strand at 1992.895 kb on + strand, within Pf1N1B4_1743 at 1992.896 kb on - strand, within Pf1N1B4_1743 at 1992.896 kb on - strand, within Pf1N1B4_1743
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 1,990,543 + Pf1N1B4_1741 0.47 -0.5 1,990,544 - Pf1N1B4_1741 0.47 -0.7 1,991,104 - Pf1N1B4_1741 0.83 -0.5 1,991,118 + Pf1N1B4_1741 0.84 -0.1 1,991,149 - Pf1N1B4_1741 0.86 -1.3 1,991,154 + Pf1N1B4_1741 0.86 +0.3 1,991,154 + Pf1N1B4_1741 0.86 -0.1 1,991,154 + Pf1N1B4_1741 0.86 -0.7 1,991,387 - -0.1 1,991,470 + +0.1 1,991,470 + +0.1 1,991,470 + +0.1 1,991,470 + -0.2 1,991,471 - +0.7 1,991,471 - +0.3 1,991,471 - +1.7 1,991,471 - +0.2 1,991,473 - -0.2 1,991,491 + +0.7 1,991,491 + -0.0 1,991,540 + +0.8 1,991,540 + +1.1 1,991,541 - -0.6 1,991,541 - -0.7 1,991,629 + Pf1N1B4_1742 0.17 -0.9 1,991,629 + +1.1 1,991,694 + Pf1N1B4_1742 0.29 -0.9 1,991,886 - +1.7 1,991,886 - Pf1N1B4_1742 0.66 -0.8 1,991,886 - Pf1N1B4_1742 0.66 +0.2 1,991,977 + Pf1N1B4_1742 0.83 -0.8 1,991,978 - Pf1N1B4_1742 0.83 +0.8 1,991,978 - Pf1N1B4_1742 0.83 -0.9 1,991,978 - Pf1N1B4_1742 0.83 -1.0 1,991,978 - Pf1N1B4_1742 0.83 -1.1 1,991,978 - Pf1N1B4_1742 0.83 -0.2 1,991,981 + Pf1N1B4_1742 0.84 -0.5 1,991,981 + Pf1N1B4_1742 0.84 -2.3 1,991,982 - Pf1N1B4_1742 0.84 -1.9 1,991,982 - Pf1N1B4_1742 0.84 +0.7 1,991,982 - Pf1N1B4_1742 0.84 -2.0 1,991,982 - Pf1N1B4_1742 0.84 +0.1 1,991,982 - Pf1N1B4_1742 0.84 +0.3 1,992,183 - -0.8 1,992,183 - +0.5 1,992,895 + Pf1N1B4_1743 0.61 +1.2 1,992,896 - Pf1N1B4_1743 0.61 -0.6 1,992,896 - Pf1N1B4_1743 0.61 -0.1
Or see this region's nucleotide sequence