Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS00910 and LRK54_RS00915 are separated by 301 nucleotides LRK54_RS00915 and LRK54_RS00920 are separated by 58 nucleotides LRK54_RS00920 and LRK54_RS00925 overlap by 4 nucleotides
LRK54_RS00910: LRK54_RS00910 - siroheme synthase CysG, at 200,471 to 201,931
_RS00910
LRK54_RS00915: LRK54_RS00915 - LysR substrate-binding domain-containing protein, at 202,233 to 203,228
_RS00915
LRK54_RS00920: LRK54_RS00920 - phosphoadenylyl-sulfate reductase, at 203,287 to 204,009
_RS00920
LRK54_RS00925: LRK54_RS00925 - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 204,006 to 205,703
_RS00925
Position (kb) 202
203
204 Strain fitness (log2 ratio) -2
-1
0
1
2 at 201.921 kb on + strand at 201.921 kb on + strand at 201.921 kb on + strand at 201.921 kb on + strand at 201.921 kb on + strand at 201.922 kb on - strand at 201.922 kb on - strand at 201.922 kb on - strand at 201.922 kb on - strand at 201.922 kb on - strand at 201.922 kb on - strand at 201.975 kb on - strand at 201.975 kb on - strand at 202.019 kb on + strand at 202.019 kb on + strand at 202.019 kb on + strand at 202.019 kb on + strand at 202.020 kb on - strand at 202.020 kb on - strand at 202.020 kb on - strand at 202.036 kb on + strand at 202.036 kb on + strand at 202.036 kb on + strand at 202.036 kb on + strand at 202.036 kb on + strand at 202.037 kb on - strand at 202.037 kb on - strand at 202.037 kb on - strand at 202.037 kb on - strand at 202.037 kb on - strand at 202.077 kb on - strand at 202.077 kb on - strand at 202.077 kb on - strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.082 kb on + strand at 202.083 kb on - strand at 202.083 kb on - strand at 202.083 kb on - strand at 202.084 kb on + strand at 202.084 kb on + strand at 202.084 kb on + strand at 202.084 kb on + strand at 202.085 kb on - strand at 202.085 kb on - strand at 202.085 kb on - strand at 202.085 kb on - strand at 202.094 kb on + strand at 202.094 kb on + strand at 202.174 kb on + strand at 202.174 kb on + strand at 202.174 kb on + strand at 202.174 kb on + strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.175 kb on - strand at 202.254 kb on + strand at 202.722 kb on + strand, within LRK54_RS00915 at 202.723 kb on - strand, within LRK54_RS00915 at 202.723 kb on - strand, within LRK54_RS00915 at 202.824 kb on + strand, within LRK54_RS00915 at 202.824 kb on + strand, within LRK54_RS00915 at 202.824 kb on + strand, within LRK54_RS00915 at 202.824 kb on + strand, within LRK54_RS00915 at 202.824 kb on + strand, within LRK54_RS00915 at 202.825 kb on - strand, within LRK54_RS00915 at 202.825 kb on - strand, within LRK54_RS00915 at 202.825 kb on - strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.932 kb on + strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 202.933 kb on - strand, within LRK54_RS00915 at 203.079 kb on + strand, within LRK54_RS00915 at 203.079 kb on + strand, within LRK54_RS00915 at 203.079 kb on + strand, within LRK54_RS00915 at 203.079 kb on + strand, within LRK54_RS00915 at 203.079 kb on + strand, within LRK54_RS00915 at 203.079 kb on + strand, within LRK54_RS00915 at 203.080 kb on - strand, within LRK54_RS00915 at 203.080 kb on - strand, within LRK54_RS00915 at 203.080 kb on - strand, within LRK54_RS00915 at 203.080 kb on - strand, within LRK54_RS00915 at 203.080 kb on - strand, within LRK54_RS00915 at 203.080 kb on - strand, within LRK54_RS00915 at 203.080 kb on - strand, within LRK54_RS00915 at 203.080 kb on - strand, within LRK54_RS00915 at 203.080 kb on - strand, within LRK54_RS00915 at 203.288 kb on + strand at 203.288 kb on + strand at 203.288 kb on + strand at 203.288 kb on + strand at 203.289 kb on - strand at 203.289 kb on - strand at 203.399 kb on + strand, within LRK54_RS00920 at 203.399 kb on + strand, within LRK54_RS00920 at 203.399 kb on + strand, within LRK54_RS00920 at 203.399 kb on + strand, within LRK54_RS00920 at 203.400 kb on - strand, within LRK54_RS00920 at 203.400 kb on - strand, within LRK54_RS00920 at 203.400 kb on - strand, within LRK54_RS00920 at 203.400 kb on - strand, within LRK54_RS00920 at 203.400 kb on - strand, within LRK54_RS00920 at 203.400 kb on - strand, within LRK54_RS00920 at 203.400 kb on - strand, within LRK54_RS00920 at 203.400 kb on - strand, within LRK54_RS00920 at 203.400 kb on - strand, within LRK54_RS00920 at 203.423 kb on + strand, within LRK54_RS00920 at 203.423 kb on + strand, within LRK54_RS00920 at 203.424 kb on - strand, within LRK54_RS00920 at 203.424 kb on - strand, within LRK54_RS00920 at 203.447 kb on + strand, within LRK54_RS00920 at 203.447 kb on + strand, within LRK54_RS00920 at 203.447 kb on + strand, within LRK54_RS00920 at 203.447 kb on + strand, within LRK54_RS00920 at 203.447 kb on + strand, within LRK54_RS00920 at 203.448 kb on - strand, within LRK54_RS00920 at 203.448 kb on - strand, within LRK54_RS00920 at 203.448 kb on - strand, within LRK54_RS00920 at 203.448 kb on - strand, within LRK54_RS00920 at 203.633 kb on + strand, within LRK54_RS00920 at 203.633 kb on + strand, within LRK54_RS00920 at 203.633 kb on + strand, within LRK54_RS00920 at 203.633 kb on + strand, within LRK54_RS00920 at 203.633 kb on + strand, within LRK54_RS00920 at 203.633 kb on + strand, within LRK54_RS00920 at 203.633 kb on + strand, within LRK54_RS00920 at 203.633 kb on + strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.634 kb on - strand, within LRK54_RS00920 at 203.714 kb on + strand, within LRK54_RS00920 at 203.714 kb on + strand, within LRK54_RS00920 at 203.715 kb on - strand, within LRK54_RS00920 at 203.715 kb on - strand, within LRK54_RS00920 at 203.715 kb on - strand, within LRK54_RS00920 at 203.765 kb on + strand, within LRK54_RS00920 at 203.765 kb on + strand, within LRK54_RS00920 at 203.765 kb on + strand, within LRK54_RS00920 at 203.765 kb on + strand, within LRK54_RS00920 at 203.765 kb on + strand, within LRK54_RS00920 at 203.765 kb on + strand, within LRK54_RS00920 at 203.765 kb on + strand, within LRK54_RS00920 at 203.765 kb on + strand, within LRK54_RS00920 at 203.765 kb on + strand, within LRK54_RS00920 at 203.766 kb on - strand, within LRK54_RS00920 at 203.766 kb on - strand, within LRK54_RS00920 at 203.766 kb on - strand, within LRK54_RS00920 at 203.766 kb on - strand, within LRK54_RS00920 at 203.766 kb on - strand, within LRK54_RS00920 at 203.783 kb on + strand, within LRK54_RS00920 at 203.784 kb on - strand, within LRK54_RS00920 at 204.112 kb on + strand at 204.113 kb on - strand at 204.113 kb on - strand at 204.172 kb on + strand at 204.173 kb on - strand at 204.224 kb on - strand, within LRK54_RS00925 at 204.224 kb on - strand, within LRK54_RS00925
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 201,921 + -1.0 201,921 + -1.3 201,921 + -0.6 201,921 + -0.5 201,921 + -0.4 201,922 - +1.6 201,922 - +0.2 201,922 - -1.8 201,922 - -0.5 201,922 - +0.1 201,922 - -2.0 201,975 - -0.7 201,975 - -0.4 202,019 + -1.5 202,019 + -0.5 202,019 + -0.8 202,019 + +0.2 202,020 - -1.5 202,020 - +0.1 202,020 - -0.3 202,036 + +0.5 202,036 + +0.2 202,036 + -0.3 202,036 + -0.9 202,036 + -0.7 202,037 - -0.6 202,037 - +0.0 202,037 - -0.7 202,037 - -1.0 202,037 - -1.3 202,077 - +0.3 202,077 - -0.3 202,077 - +0.2 202,082 + -0.6 202,082 + -1.9 202,082 + -0.3 202,082 + +0.5 202,082 + -1.6 202,082 + -0.8 202,082 + -0.6 202,082 + +1.5 202,083 - -0.1 202,083 - +1.2 202,083 - -0.2 202,084 + -0.6 202,084 + -0.3 202,084 + -0.2 202,084 + -1.3 202,085 - -0.1 202,085 - +0.1 202,085 - -0.9 202,085 - -1.2 202,094 + +0.8 202,094 + -1.5 202,174 + -0.6 202,174 + -0.5 202,174 + -0.8 202,174 + +0.4 202,175 - -0.1 202,175 - +0.4 202,175 - -1.0 202,175 - -0.8 202,175 - -0.1 202,175 - -0.0 202,175 - +0.1 202,175 - -0.7 202,175 - -0.4 202,175 - -0.7 202,254 + -0.2 202,722 + LRK54_RS00915 0.49 +0.6 202,723 - LRK54_RS00915 0.49 +0.6 202,723 - LRK54_RS00915 0.49 +0.4 202,824 + LRK54_RS00915 0.59 +0.6 202,824 + LRK54_RS00915 0.59 -0.6 202,824 + LRK54_RS00915 0.59 +0.9 202,824 + LRK54_RS00915 0.59 +0.5 202,824 + LRK54_RS00915 0.59 +0.3 202,825 - LRK54_RS00915 0.59 +0.5 202,825 - LRK54_RS00915 0.59 +0.6 202,825 - LRK54_RS00915 0.59 -0.0 202,932 + LRK54_RS00915 0.70 +0.1 202,932 + LRK54_RS00915 0.70 -0.2 202,932 + LRK54_RS00915 0.70 +1.0 202,932 + LRK54_RS00915 0.70 +0.9 202,932 + LRK54_RS00915 0.70 -0.3 202,932 + LRK54_RS00915 0.70 +0.2 202,932 + LRK54_RS00915 0.70 +0.9 202,932 + LRK54_RS00915 0.70 -1.5 202,932 + LRK54_RS00915 0.70 +0.5 202,932 + LRK54_RS00915 0.70 +0.9 202,932 + LRK54_RS00915 0.70 -0.2 202,932 + LRK54_RS00915 0.70 +0.2 202,932 + LRK54_RS00915 0.70 -0.5 202,932 + LRK54_RS00915 0.70 +0.9 202,933 - LRK54_RS00915 0.70 +0.1 202,933 - LRK54_RS00915 0.70 -0.5 202,933 - LRK54_RS00915 0.70 -0.3 202,933 - LRK54_RS00915 0.70 +0.1 202,933 - LRK54_RS00915 0.70 +1.3 202,933 - LRK54_RS00915 0.70 +0.4 202,933 - LRK54_RS00915 0.70 +0.8 202,933 - LRK54_RS00915 0.70 +1.5 202,933 - LRK54_RS00915 0.70 +0.5 202,933 - LRK54_RS00915 0.70 -0.6 202,933 - LRK54_RS00915 0.70 +0.3 202,933 - LRK54_RS00915 0.70 -0.2 202,933 - LRK54_RS00915 0.70 +0.5 203,079 + LRK54_RS00915 0.85 -0.2 203,079 + LRK54_RS00915 0.85 +0.5 203,079 + LRK54_RS00915 0.85 +1.0 203,079 + LRK54_RS00915 0.85 +0.7 203,079 + LRK54_RS00915 0.85 -0.2 203,079 + LRK54_RS00915 0.85 +0.2 203,080 - LRK54_RS00915 0.85 +0.7 203,080 - LRK54_RS00915 0.85 +0.3 203,080 - LRK54_RS00915 0.85 +0.6 203,080 - LRK54_RS00915 0.85 -0.4 203,080 - LRK54_RS00915 0.85 +0.5 203,080 - LRK54_RS00915 0.85 +0.3 203,080 - LRK54_RS00915 0.85 +0.2 203,080 - LRK54_RS00915 0.85 +2.1 203,080 - LRK54_RS00915 0.85 +2.6 203,288 + -0.1 203,288 + -0.4 203,288 + -0.0 203,288 + +0.5 203,289 - +0.1 203,289 - -0.2 203,399 + LRK54_RS00920 0.15 -0.3 203,399 + LRK54_RS00920 0.15 +1.0 203,399 + LRK54_RS00920 0.15 -0.3 203,399 + LRK54_RS00920 0.15 -1.0 203,400 - LRK54_RS00920 0.16 +0.0 203,400 - LRK54_RS00920 0.16 +0.1 203,400 - LRK54_RS00920 0.16 +0.3 203,400 - LRK54_RS00920 0.16 +0.1 203,400 - LRK54_RS00920 0.16 -0.0 203,400 - LRK54_RS00920 0.16 +1.2 203,400 - LRK54_RS00920 0.16 -0.6 203,400 - LRK54_RS00920 0.16 +0.2 203,400 - LRK54_RS00920 0.16 -0.5 203,423 + LRK54_RS00920 0.19 +0.7 203,423 + LRK54_RS00920 0.19 +0.3 203,424 - LRK54_RS00920 0.19 +0.2 203,424 - LRK54_RS00920 0.19 +0.1 203,447 + LRK54_RS00920 0.22 +0.4 203,447 + LRK54_RS00920 0.22 -0.6 203,447 + LRK54_RS00920 0.22 -0.7 203,447 + LRK54_RS00920 0.22 +0.1 203,447 + LRK54_RS00920 0.22 +0.1 203,448 - LRK54_RS00920 0.22 +1.6 203,448 - LRK54_RS00920 0.22 +0.9 203,448 - LRK54_RS00920 0.22 +0.2 203,448 - LRK54_RS00920 0.22 +0.4 203,633 + LRK54_RS00920 0.48 +0.2 203,633 + LRK54_RS00920 0.48 +0.5 203,633 + LRK54_RS00920 0.48 -0.3 203,633 + LRK54_RS00920 0.48 -0.1 203,633 + LRK54_RS00920 0.48 -0.2 203,633 + LRK54_RS00920 0.48 +0.5 203,633 + LRK54_RS00920 0.48 +0.7 203,633 + LRK54_RS00920 0.48 -0.1 203,634 - LRK54_RS00920 0.48 -0.2 203,634 - LRK54_RS00920 0.48 -0.9 203,634 - LRK54_RS00920 0.48 -0.1 203,634 - LRK54_RS00920 0.48 +1.0 203,634 - LRK54_RS00920 0.48 -0.1 203,634 - LRK54_RS00920 0.48 +0.3 203,634 - LRK54_RS00920 0.48 +1.7 203,634 - LRK54_RS00920 0.48 -0.2 203,634 - LRK54_RS00920 0.48 -0.0 203,634 - LRK54_RS00920 0.48 +0.6 203,634 - LRK54_RS00920 0.48 -1.6 203,634 - LRK54_RS00920 0.48 -0.8 203,634 - LRK54_RS00920 0.48 -0.5 203,634 - LRK54_RS00920 0.48 +0.3 203,714 + LRK54_RS00920 0.59 +1.0 203,714 + LRK54_RS00920 0.59 -0.1 203,715 - LRK54_RS00920 0.59 -0.0 203,715 - LRK54_RS00920 0.59 -0.1 203,715 - LRK54_RS00920 0.59 -0.1 203,765 + LRK54_RS00920 0.66 -0.6 203,765 + LRK54_RS00920 0.66 +0.1 203,765 + LRK54_RS00920 0.66 -0.2 203,765 + LRK54_RS00920 0.66 -0.8 203,765 + LRK54_RS00920 0.66 -0.1 203,765 + LRK54_RS00920 0.66 -0.2 203,765 + LRK54_RS00920 0.66 +1.4 203,765 + LRK54_RS00920 0.66 +0.2 203,765 + LRK54_RS00920 0.66 +0.0 203,766 - LRK54_RS00920 0.66 +0.0 203,766 - LRK54_RS00920 0.66 -1.5 203,766 - LRK54_RS00920 0.66 -0.6 203,766 - LRK54_RS00920 0.66 -0.1 203,766 - LRK54_RS00920 0.66 +0.5 203,783 + LRK54_RS00920 0.69 -2.4 203,784 - LRK54_RS00920 0.69 -0.1 204,112 + -0.0 204,113 - -0.6 204,113 - -0.3 204,172 + +0.3 204,173 - +0.3 204,224 - LRK54_RS00925 0.13 -1.1 204,224 - LRK54_RS00925 0.13 -0.2
Or see this region's nucleotide sequence