Experiment: R2A with Zinc 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06305 and CA265_RS06310 are separated by 80 nucleotides CA265_RS06310 and CA265_RS06315 are separated by 137 nucleotides CA265_RS06315 and CA265_RS06320 are separated by 2 nucleotides
CA265_RS06305: CA265_RS06305 - succinyl-CoA--3-ketoacid-CoA transferase, at 1,549,306 to 1,550,010
_RS06305
CA265_RS06310: CA265_RS06310 - succinyl-CoA--3-ketoacid-CoA transferase, at 1,550,091 to 1,550,750
_RS06310
CA265_RS06315: CA265_RS06315 - uridine kinase, at 1,550,888 to 1,551,532
_RS06315
CA265_RS06320: CA265_RS06320 - hypothetical protein, at 1,551,535 to 1,553,073
_RS06320
Position (kb)
1550
1551 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1549.146 kb on - strand at 1549.152 kb on - strand at 1549.152 kb on - strand at 1549.314 kb on + strand at 1549.314 kb on + strand at 1549.315 kb on - strand at 1549.315 kb on - strand at 1549.353 kb on - strand at 1549.464 kb on + strand, within CA265_RS06305 at 1549.537 kb on + strand, within CA265_RS06305 at 1549.538 kb on - strand, within CA265_RS06305 at 1549.538 kb on - strand, within CA265_RS06305 at 1549.592 kb on + strand at 1549.616 kb on + strand, within CA265_RS06305 at 1549.770 kb on + strand, within CA265_RS06305 at 1549.771 kb on - strand, within CA265_RS06305 at 1549.914 kb on + strand, within CA265_RS06305 at 1549.915 kb on - strand, within CA265_RS06305 at 1549.920 kb on + strand, within CA265_RS06305 at 1549.993 kb on - strand at 1550.051 kb on - strand at 1550.086 kb on + strand at 1550.086 kb on + strand at 1550.148 kb on + strand at 1550.148 kb on + strand at 1550.149 kb on - strand at 1550.149 kb on - strand at 1550.208 kb on - strand, within CA265_RS06310 at 1550.309 kb on + strand, within CA265_RS06310 at 1550.331 kb on - strand, within CA265_RS06310 at 1550.331 kb on - strand, within CA265_RS06310 at 1550.384 kb on - strand, within CA265_RS06310 at 1550.440 kb on + strand, within CA265_RS06310 at 1550.525 kb on + strand, within CA265_RS06310 at 1550.526 kb on - strand, within CA265_RS06310 at 1550.526 kb on - strand, within CA265_RS06310 at 1550.526 kb on - strand, within CA265_RS06310 at 1550.603 kb on - strand, within CA265_RS06310 at 1550.671 kb on + strand, within CA265_RS06310 at 1550.671 kb on + strand, within CA265_RS06310 at 1550.672 kb on - strand, within CA265_RS06310 at 1550.697 kb on - strand at 1550.714 kb on + strand at 1550.766 kb on + strand at 1550.794 kb on + strand at 1550.822 kb on + strand at 1550.834 kb on - strand at 1550.840 kb on - strand at 1550.886 kb on - strand at 1550.894 kb on + strand at 1550.895 kb on - strand at 1550.895 kb on - strand at 1551.014 kb on + strand, within CA265_RS06315 at 1551.015 kb on - strand, within CA265_RS06315 at 1551.018 kb on + strand, within CA265_RS06315 at 1551.018 kb on + strand, within CA265_RS06315 at 1551.058 kb on - strand, within CA265_RS06315 at 1551.063 kb on - strand, within CA265_RS06315 at 1551.104 kb on + strand, within CA265_RS06315 at 1551.116 kb on + strand, within CA265_RS06315 at 1551.151 kb on + strand, within CA265_RS06315 at 1551.159 kb on - strand, within CA265_RS06315 at 1551.211 kb on + strand, within CA265_RS06315 at 1551.212 kb on - strand, within CA265_RS06315 at 1551.268 kb on + strand, within CA265_RS06315 at 1551.405 kb on + strand, within CA265_RS06315 at 1551.408 kb on + strand, within CA265_RS06315 at 1551.468 kb on - strand at 1551.468 kb on - strand at 1551.512 kb on + strand at 1551.547 kb on + strand at 1551.547 kb on - strand at 1551.547 kb on - strand at 1551.554 kb on - strand at 1551.606 kb on + strand at 1551.678 kb on + strand at 1551.705 kb on + strand, within CA265_RS06320 at 1551.705 kb on + strand, within CA265_RS06320 at 1551.718 kb on + strand, within CA265_RS06320 at 1551.719 kb on - strand, within CA265_RS06320
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc 0.2 mM remove 1,549,146 - +0.6 1,549,152 - -2.2 1,549,152 - +0.2 1,549,314 + +0.0 1,549,314 + +0.9 1,549,315 - +0.8 1,549,315 - +0.3 1,549,353 - -1.2 1,549,464 + CA265_RS06305 0.22 -0.6 1,549,537 + CA265_RS06305 0.33 +0.2 1,549,538 - CA265_RS06305 0.33 +0.1 1,549,538 - CA265_RS06305 0.33 +0.6 1,549,592 + +0.6 1,549,616 + CA265_RS06305 0.44 +0.3 1,549,770 + CA265_RS06305 0.66 -0.5 1,549,771 - CA265_RS06305 0.66 +0.4 1,549,914 + CA265_RS06305 0.86 +1.6 1,549,915 - CA265_RS06305 0.86 -0.1 1,549,920 + CA265_RS06305 0.87 +0.4 1,549,993 - -0.2 1,550,051 - -0.5 1,550,086 + +0.0 1,550,086 + +0.7 1,550,148 + +0.0 1,550,148 + -0.5 1,550,149 - +0.3 1,550,149 - +0.5 1,550,208 - CA265_RS06310 0.18 -0.9 1,550,309 + CA265_RS06310 0.33 -0.3 1,550,331 - CA265_RS06310 0.36 +1.1 1,550,331 - CA265_RS06310 0.36 +0.6 1,550,384 - CA265_RS06310 0.44 -0.4 1,550,440 + CA265_RS06310 0.53 +0.7 1,550,525 + CA265_RS06310 0.66 -0.4 1,550,526 - CA265_RS06310 0.66 -0.0 1,550,526 - CA265_RS06310 0.66 -1.5 1,550,526 - CA265_RS06310 0.66 -1.5 1,550,603 - CA265_RS06310 0.78 -0.1 1,550,671 + CA265_RS06310 0.88 -0.1 1,550,671 + CA265_RS06310 0.88 +1.5 1,550,672 - CA265_RS06310 0.88 +0.5 1,550,697 - +0.5 1,550,714 + +1.2 1,550,766 + +0.2 1,550,794 + +0.5 1,550,822 + +0.7 1,550,834 - +1.9 1,550,840 - -2.0 1,550,886 - -0.5 1,550,894 + +3.1 1,550,895 - -0.5 1,550,895 - +1.1 1,551,014 + CA265_RS06315 0.20 +1.5 1,551,015 - CA265_RS06315 0.20 +1.6 1,551,018 + CA265_RS06315 0.20 +0.4 1,551,018 + CA265_RS06315 0.20 +1.4 1,551,058 - CA265_RS06315 0.26 +0.6 1,551,063 - CA265_RS06315 0.27 +0.4 1,551,104 + CA265_RS06315 0.33 +1.3 1,551,116 + CA265_RS06315 0.35 +0.6 1,551,151 + CA265_RS06315 0.41 -0.1 1,551,159 - CA265_RS06315 0.42 +1.5 1,551,211 + CA265_RS06315 0.50 +1.3 1,551,212 - CA265_RS06315 0.50 -0.0 1,551,268 + CA265_RS06315 0.59 +0.6 1,551,405 + CA265_RS06315 0.80 +1.2 1,551,408 + CA265_RS06315 0.81 +0.4 1,551,468 - +1.5 1,551,468 - +2.0 1,551,512 + +1.5 1,551,547 + -1.4 1,551,547 - -0.8 1,551,547 - -1.9 1,551,554 - -0.5 1,551,606 + +1.2 1,551,678 + +1.8 1,551,705 + CA265_RS06320 0.11 +0.5 1,551,705 + CA265_RS06320 0.11 -0.2 1,551,718 + CA265_RS06320 0.12 -0.3 1,551,719 - CA265_RS06320 0.12 -1.7
Or see this region's nucleotide sequence