Experiment: KB with Carbenicillin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3056 and Psyr_3057 overlap by 23 nucleotides Psyr_3057 and Psyr_3058 are separated by 2 nucleotides Psyr_3058 and Psyr_3059 are separated by 312 nucleotides
Psyr_3056: Psyr_3056 - Amidase, hydantoinase/carbamoylase, at 3,653,505 to 3,654,785
_3056
Psyr_3057: Psyr_3057 - transcriptional regulator, DeoR family, at 3,654,763 to 3,655,599
_3057
Psyr_3058: Psyr_3058 - Amidase, at 3,655,602 to 3,657,005
_3058
Psyr_3059: Psyr_3059 - Hemolysin-type calcium-binding region:Peptidase M10A and M12B, matrixin and adamalysin, at 3,657,318 to 3,658,925
_3059
Position (kb)
3655
3656
3657
3658 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3654.636 kb on + strand, within Psyr_3056 at 3654.636 kb on + strand, within Psyr_3056 at 3654.636 kb on + strand, within Psyr_3056 at 3654.636 kb on + strand, within Psyr_3056 at 3654.637 kb on - strand, within Psyr_3056 at 3654.637 kb on - strand, within Psyr_3056 at 3654.637 kb on - strand, within Psyr_3056 at 3654.637 kb on - strand, within Psyr_3056 at 3654.637 kb on - strand, within Psyr_3056 at 3654.770 kb on + strand at 3654.873 kb on + strand, within Psyr_3057 at 3654.972 kb on - strand, within Psyr_3057 at 3655.603 kb on + strand at 3655.604 kb on - strand at 3655.653 kb on + strand at 3655.653 kb on + strand at 3655.654 kb on - strand at 3655.786 kb on + strand, within Psyr_3058 at 3655.787 kb on - strand, within Psyr_3058 at 3655.907 kb on - strand, within Psyr_3058 at 3656.003 kb on + strand, within Psyr_3058 at 3656.618 kb on + strand, within Psyr_3058 at 3656.619 kb on - strand, within Psyr_3058 at 3656.619 kb on - strand, within Psyr_3058 at 3656.797 kb on - strand, within Psyr_3058 at 3656.878 kb on + strand at 3656.878 kb on + strand at 3656.878 kb on + strand at 3656.879 kb on - strand at 3656.879 kb on - strand at 3656.879 kb on - strand at 3657.037 kb on - strand at 3657.051 kb on + strand at 3657.052 kb on - strand at 3657.052 kb on - strand at 3657.088 kb on + strand at 3657.833 kb on + strand, within Psyr_3059 at 3657.834 kb on - strand, within Psyr_3059 at 3657.834 kb on - strand, within Psyr_3059 at 3657.834 kb on - strand, within Psyr_3059 at 3657.842 kb on - strand, within Psyr_3059 at 3657.842 kb on - strand, within Psyr_3059 at 3657.842 kb on - strand, within Psyr_3059 at 3657.919 kb on + strand, within Psyr_3059 at 3657.919 kb on + strand, within Psyr_3059 at 3657.919 kb on + strand, within Psyr_3059 at 3657.919 kb on + strand, within Psyr_3059 at 3657.919 kb on + strand, within Psyr_3059 at 3657.919 kb on + strand, within Psyr_3059 at 3657.919 kb on + strand, within Psyr_3059 at 3657.920 kb on - strand, within Psyr_3059 at 3657.920 kb on - strand, within Psyr_3059 at 3657.920 kb on - strand, within Psyr_3059 at 3657.920 kb on - strand, within Psyr_3059 at 3657.920 kb on - strand, within Psyr_3059 at 3657.920 kb on - strand, within Psyr_3059 at 3657.920 kb on - strand, within Psyr_3059 at 3657.924 kb on - strand, within Psyr_3059 at 3657.924 kb on - strand, within Psyr_3059
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Carbenicillin 250 ug/ml remove 3,654,636 + Psyr_3056 0.88 +0.4 3,654,636 + Psyr_3056 0.88 +0.3 3,654,636 + Psyr_3056 0.88 +0.0 3,654,636 + Psyr_3056 0.88 +0.0 3,654,637 - Psyr_3056 0.88 -1.5 3,654,637 - Psyr_3056 0.88 -0.0 3,654,637 - Psyr_3056 0.88 -0.3 3,654,637 - Psyr_3056 0.88 -2.5 3,654,637 - Psyr_3056 0.88 -1.3 3,654,770 + +1.0 3,654,873 + Psyr_3057 0.13 -0.6 3,654,972 - Psyr_3057 0.25 +1.7 3,655,603 + +0.7 3,655,604 - +1.2 3,655,653 + +1.8 3,655,653 + -0.9 3,655,654 - -0.2 3,655,786 + Psyr_3058 0.13 -0.5 3,655,787 - Psyr_3058 0.13 +0.4 3,655,907 - Psyr_3058 0.22 -0.7 3,656,003 + Psyr_3058 0.29 -0.7 3,656,618 + Psyr_3058 0.72 -1.3 3,656,619 - Psyr_3058 0.72 -0.6 3,656,619 - Psyr_3058 0.72 -0.6 3,656,797 - Psyr_3058 0.85 -1.6 3,656,878 + -0.3 3,656,878 + +0.6 3,656,878 + -0.3 3,656,879 - +1.0 3,656,879 - -0.2 3,656,879 - +0.3 3,657,037 - +2.0 3,657,051 + +1.3 3,657,052 - -0.5 3,657,052 - -1.1 3,657,088 + -0.5 3,657,833 + Psyr_3059 0.32 +0.2 3,657,834 - Psyr_3059 0.32 +2.0 3,657,834 - Psyr_3059 0.32 +0.3 3,657,834 - Psyr_3059 0.32 +0.9 3,657,842 - Psyr_3059 0.33 +0.7 3,657,842 - Psyr_3059 0.33 +0.5 3,657,842 - Psyr_3059 0.33 +0.1 3,657,919 + Psyr_3059 0.37 +0.5 3,657,919 + Psyr_3059 0.37 +1.3 3,657,919 + Psyr_3059 0.37 -0.3 3,657,919 + Psyr_3059 0.37 -0.6 3,657,919 + Psyr_3059 0.37 +0.2 3,657,919 + Psyr_3059 0.37 -0.5 3,657,919 + Psyr_3059 0.37 -0.3 3,657,920 - Psyr_3059 0.37 -0.5 3,657,920 - Psyr_3059 0.37 -0.1 3,657,920 - Psyr_3059 0.37 -0.1 3,657,920 - Psyr_3059 0.37 +0.2 3,657,920 - Psyr_3059 0.37 +0.7 3,657,920 - Psyr_3059 0.37 -0.3 3,657,920 - Psyr_3059 0.37 -0.3 3,657,924 - Psyr_3059 0.38 +0.2 3,657,924 - Psyr_3059 0.38 +0.5
Or see this region's nucleotide sequence