Experiment: KB with Carbenicillin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1948 and Psyr_1949 are separated by 0 nucleotides Psyr_1949 and Psyr_1950 overlap by 1 nucleotides Psyr_1950 and Psyr_1951 overlap by 4 nucleotides
Psyr_1948: Psyr_1948 - ABC transporter, periplasmic substrate-binding protein, putative, at 2,233,389 to 2,234,306
_1948
Psyr_1949: Psyr_1949 - ABC-3, at 2,234,307 to 2,235,206
_1949
Psyr_1950: Psyr_1950 - ABC transporter, at 2,235,206 to 2,235,970
_1950
Psyr_1951: Psyr_1951 - Periplasmic solute binding protein, at 2,235,967 to 2,236,899
_1951
Position (kb)
2234
2235
2236 Strain fitness (log2 ratio)
-1
0
1
2 at 2233.374 kb on + strand at 2233.663 kb on + strand at 2233.663 kb on + strand, within Psyr_1948 at 2233.663 kb on + strand, within Psyr_1948 at 2233.701 kb on - strand, within Psyr_1948 at 2233.747 kb on - strand, within Psyr_1948 at 2233.850 kb on + strand, within Psyr_1948 at 2233.967 kb on + strand, within Psyr_1948 at 2233.968 kb on - strand, within Psyr_1948 at 2234.161 kb on - strand, within Psyr_1948 at 2234.161 kb on - strand, within Psyr_1948 at 2234.177 kb on - strand, within Psyr_1948 at 2234.291 kb on + strand at 2234.291 kb on + strand at 2234.292 kb on - strand at 2234.292 kb on - strand at 2234.295 kb on + strand at 2234.295 kb on + strand at 2234.309 kb on - strand at 2234.615 kb on - strand, within Psyr_1949 at 2234.689 kb on + strand, within Psyr_1949 at 2234.854 kb on - strand, within Psyr_1949 at 2234.912 kb on - strand, within Psyr_1949 at 2234.959 kb on + strand, within Psyr_1949 at 2234.971 kb on + strand, within Psyr_1949 at 2234.972 kb on - strand, within Psyr_1949 at 2235.721 kb on - strand, within Psyr_1950 at 2236.056 kb on - strand at 2236.056 kb on - strand at 2236.115 kb on + strand, within Psyr_1951 at 2236.116 kb on - strand, within Psyr_1951 at 2236.116 kb on - strand, within Psyr_1951
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Carbenicillin 250 ug/ml remove 2,233,374 + -0.6 2,233,663 + +0.7 2,233,663 + Psyr_1948 0.30 +0.7 2,233,663 + Psyr_1948 0.30 +0.3 2,233,701 - Psyr_1948 0.34 -0.1 2,233,747 - Psyr_1948 0.39 -0.2 2,233,850 + Psyr_1948 0.50 -0.3 2,233,967 + Psyr_1948 0.63 -0.3 2,233,968 - Psyr_1948 0.63 +0.5 2,234,161 - Psyr_1948 0.84 +1.4 2,234,161 - Psyr_1948 0.84 +1.0 2,234,177 - Psyr_1948 0.86 +0.8 2,234,291 + +0.5 2,234,291 + +0.4 2,234,292 - -0.3 2,234,292 - -1.3 2,234,295 + +0.5 2,234,295 + +0.3 2,234,309 - -1.1 2,234,615 - Psyr_1949 0.34 +1.0 2,234,689 + Psyr_1949 0.42 -0.9 2,234,854 - Psyr_1949 0.61 -1.1 2,234,912 - Psyr_1949 0.67 -0.5 2,234,959 + Psyr_1949 0.72 -1.6 2,234,971 + Psyr_1949 0.74 -0.6 2,234,972 - Psyr_1949 0.74 -0.5 2,235,721 - Psyr_1950 0.67 +0.8 2,236,056 - -1.3 2,236,056 - +1.8 2,236,115 + Psyr_1951 0.16 +2.7 2,236,116 - Psyr_1951 0.16 -0.1 2,236,116 - Psyr_1951 0.16 -0.9
Or see this region's nucleotide sequence