Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0212

Experiment: KB with Carbenicillin 250 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0210 and Psyr_0211 are separated by 147 nucleotidesPsyr_0211 and Psyr_0212 are separated by 13 nucleotidesPsyr_0212 and Psyr_0213 are separated by 312 nucleotides Psyr_0210: Psyr_0210 - DNA-directed RNA polymerase subunit omega, at 225,170 to 225,433 _0210 Psyr_0211: Psyr_0211 - guanylate kinase, at 225,581 to 226,225 _0211 Psyr_0212: Psyr_0212 - Conserved hypothetical protein 255, at 226,239 to 227,102 _0212 Psyr_0213: Psyr_0213 - RNAse PH, at 227,415 to 228,137 _0213 Position (kb) 226 227 228Strain fitness (log2 ratio) -2 -1 0 1 2at 225.365 kb on - strand, within Psyr_0210at 225.405 kb on - strand, within Psyr_0210at 225.405 kb on - strand, within Psyr_0210at 225.549 kb on - strandat 225.612 kb on + strandat 225.613 kb on - strandat 225.613 kb on - strandat 226.341 kb on - strand, within Psyr_0212at 226.341 kb on - strand, within Psyr_0212at 226.341 kb on - strand, within Psyr_0212at 226.341 kb on - strand, within Psyr_0212at 226.341 kb on - strand, within Psyr_0212at 226.341 kb on - strand, within Psyr_0212at 226.356 kb on - strand, within Psyr_0212at 227.110 kb on - strandat 227.196 kb on - strandat 227.272 kb on + strandat 227.344 kb on + strandat 227.345 kb on - strandat 227.345 kb on - strandat 227.345 kb on - strandat 227.373 kb on + strandat 227.373 kb on + strandat 227.373 kb on + strandat 227.373 kb on + strandat 227.374 kb on - strandat 227.375 kb on + strandat 227.376 kb on - strandat 227.376 kb on - strandat 227.401 kb on + strandat 227.401 kb on + strandat 227.408 kb on - strandat 227.475 kb on + strandat 227.475 kb on + strandat 227.476 kb on - strandat 227.500 kb on + strand, within Psyr_0213at 227.501 kb on - strand, within Psyr_0213at 227.519 kb on + strand, within Psyr_0213at 227.519 kb on + strand, within Psyr_0213at 227.519 kb on + strand, within Psyr_0213at 227.558 kb on - strand, within Psyr_0213at 227.624 kb on + strand, within Psyr_0213at 227.637 kb on - strand, within Psyr_0213at 227.675 kb on + strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.676 kb on - strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.754 kb on + strand, within Psyr_0213at 227.755 kb on - strand, within Psyr_0213at 227.755 kb on - strand, within Psyr_0213at 227.755 kb on - strand, within Psyr_0213at 227.804 kb on + strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.805 kb on - strand, within Psyr_0213at 227.807 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.919 kb on + strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.920 kb on - strand, within Psyr_0213at 227.949 kb on + strand, within Psyr_0213at 227.950 kb on - strand, within Psyr_0213at 227.950 kb on - strand, within Psyr_0213

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Carbenicillin 250 ug/ml
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225,365 - Psyr_0210 0.74 -0.1
225,405 - Psyr_0210 0.89 -0.4
225,405 - Psyr_0210 0.89 -0.4
225,549 - -0.4
225,612 + +0.3
225,613 - -0.5
225,613 - -0.6
226,341 - Psyr_0212 0.12 -0.1
226,341 - Psyr_0212 0.12 +0.4
226,341 - Psyr_0212 0.12 -0.3
226,341 - Psyr_0212 0.12 -0.7
226,341 - Psyr_0212 0.12 -1.4
226,341 - Psyr_0212 0.12 -0.3
226,356 - Psyr_0212 0.14 -0.9
227,110 - -0.2
227,196 - -0.5
227,272 + -0.1
227,344 + -0.4
227,345 - -0.0
227,345 - +0.2
227,345 - -1.2
227,373 + +0.3
227,373 + +0.5
227,373 + -0.2
227,373 + +1.2
227,374 - +0.4
227,375 + +0.8
227,376 - +0.0
227,376 - +0.4
227,401 + +0.4
227,401 + -0.0
227,408 - -0.6
227,475 + +0.9
227,475 + -1.2
227,476 - +0.0
227,500 + Psyr_0213 0.12 -2.0
227,501 - Psyr_0213 0.12 +0.6
227,519 + Psyr_0213 0.14 +0.2
227,519 + Psyr_0213 0.14 +0.5
227,519 + Psyr_0213 0.14 +0.2
227,558 - Psyr_0213 0.20 -0.6
227,624 + Psyr_0213 0.29 -0.7
227,637 - Psyr_0213 0.31 +0.2
227,675 + Psyr_0213 0.36 +0.3
227,676 - Psyr_0213 0.36 -0.8
227,676 - Psyr_0213 0.36 +0.2
227,676 - Psyr_0213 0.36 -0.1
227,676 - Psyr_0213 0.36 +0.6
227,754 + Psyr_0213 0.47 +1.2
227,754 + Psyr_0213 0.47 -0.4
227,754 + Psyr_0213 0.47 +0.6
227,754 + Psyr_0213 0.47 +2.2
227,754 + Psyr_0213 0.47 -2.1
227,755 - Psyr_0213 0.47 -0.8
227,755 - Psyr_0213 0.47 -0.7
227,755 - Psyr_0213 0.47 -2.0
227,804 + Psyr_0213 0.54 -0.1
227,805 - Psyr_0213 0.54 -0.8
227,805 - Psyr_0213 0.54 -0.4
227,805 - Psyr_0213 0.54 +1.0
227,805 - Psyr_0213 0.54 +0.2
227,805 - Psyr_0213 0.54 +0.4
227,805 - Psyr_0213 0.54 +0.3
227,805 - Psyr_0213 0.54 +0.8
227,807 + Psyr_0213 0.54 -1.3
227,919 + Psyr_0213 0.70 +0.6
227,919 + Psyr_0213 0.70 -0.3
227,919 + Psyr_0213 0.70 +0.0
227,919 + Psyr_0213 0.70 -1.6
227,920 - Psyr_0213 0.70 -0.1
227,920 - Psyr_0213 0.70 +0.2
227,920 - Psyr_0213 0.70 +1.9
227,920 - Psyr_0213 0.70 -0.3
227,920 - Psyr_0213 0.70 -0.0
227,949 + Psyr_0213 0.74 +0.2
227,950 - Psyr_0213 0.74 -0.4
227,950 - Psyr_0213 0.74 -1.6

Or see this region's nucleotide sequence