Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2249 and Pf1N1B4_2250 are separated by 9 nucleotides Pf1N1B4_2250 and Pf1N1B4_2251 overlap by 4 nucleotides Pf1N1B4_2251 and Pf1N1B4_2252 are separated by 34 nucleotides
Pf1N1B4_2249 - Alkanesulfonate monooxygenase (EC 1.14.14.5), at 2,565,573 to 2,566,721
_2249
Pf1N1B4_2250 - Alkanesulfonates transport system permease protein, at 2,566,731 to 2,567,513
_2250
Pf1N1B4_2251 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 2,567,510 to 2,568,316
_2251
Pf1N1B4_2252 - Organosulfonate utilization protein SsuF, at 2,568,351 to 2,568,566
_2252
Position (kb)
2566
2567
2568 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2566.020 kb on + strand, within Pf1N1B4_2249 at 2566.021 kb on - strand, within Pf1N1B4_2249 at 2566.063 kb on - strand, within Pf1N1B4_2249 at 2566.547 kb on + strand, within Pf1N1B4_2249 at 2566.547 kb on + strand, within Pf1N1B4_2249 at 2566.547 kb on + strand, within Pf1N1B4_2249 at 2566.548 kb on - strand, within Pf1N1B4_2249 at 2566.548 kb on - strand, within Pf1N1B4_2249 at 2566.606 kb on + strand, within Pf1N1B4_2249 at 2566.606 kb on + strand, within Pf1N1B4_2249 at 2566.607 kb on - strand at 2566.657 kb on - strand at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.962 kb on - strand, within Pf1N1B4_2250 at 2567.136 kb on + strand, within Pf1N1B4_2250 at 2567.136 kb on + strand, within Pf1N1B4_2250 at 2567.137 kb on - strand, within Pf1N1B4_2250 at 2567.137 kb on - strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.199 kb on + strand, within Pf1N1B4_2250 at 2567.199 kb on + strand, within Pf1N1B4_2250 at 2567.199 kb on + strand, within Pf1N1B4_2250 at 2567.200 kb on - strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.671 kb on + strand, within Pf1N1B4_2251 at 2567.671 kb on + strand, within Pf1N1B4_2251 at 2567.672 kb on - strand, within Pf1N1B4_2251 at 2568.293 kb on - strand at 2568.314 kb on + strand at 2568.315 kb on - strand at 2568.315 kb on - strand at 2568.315 kb on - strand at 2568.319 kb on + strand at 2568.319 kb on + strand at 2568.319 kb on + strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.356 kb on + strand at 2568.357 kb on - strand at 2568.357 kb on - strand at 2568.378 kb on - strand, within Pf1N1B4_2252 at 2568.474 kb on - strand, within Pf1N1B4_2252
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 2,566,020 + Pf1N1B4_2249 0.39 -1.4 2,566,021 - Pf1N1B4_2249 0.39 -0.3 2,566,063 - Pf1N1B4_2249 0.43 -1.1 2,566,547 + Pf1N1B4_2249 0.85 +2.4 2,566,547 + Pf1N1B4_2249 0.85 -0.6 2,566,547 + Pf1N1B4_2249 0.85 -0.3 2,566,548 - Pf1N1B4_2249 0.85 -0.5 2,566,548 - Pf1N1B4_2249 0.85 -0.1 2,566,606 + Pf1N1B4_2249 0.90 +1.0 2,566,606 + Pf1N1B4_2249 0.90 -0.5 2,566,607 - -0.4 2,566,657 - -0.1 2,566,961 + Pf1N1B4_2250 0.29 -0.5 2,566,961 + Pf1N1B4_2250 0.29 +0.1 2,566,961 + Pf1N1B4_2250 0.29 -1.3 2,566,961 + Pf1N1B4_2250 0.29 -0.8 2,566,961 + Pf1N1B4_2250 0.29 -0.3 2,566,961 + Pf1N1B4_2250 0.29 +0.9 2,566,961 + Pf1N1B4_2250 0.29 +1.7 2,566,962 - Pf1N1B4_2250 0.30 +0.4 2,567,136 + Pf1N1B4_2250 0.52 +0.4 2,567,136 + Pf1N1B4_2250 0.52 -1.7 2,567,137 - Pf1N1B4_2250 0.52 +0.1 2,567,137 - Pf1N1B4_2250 0.52 +0.3 2,567,148 + Pf1N1B4_2250 0.53 -0.7 2,567,148 + Pf1N1B4_2250 0.53 +0.3 2,567,148 + Pf1N1B4_2250 0.53 +0.1 2,567,148 + Pf1N1B4_2250 0.53 +1.0 2,567,148 + Pf1N1B4_2250 0.53 +1.4 2,567,148 + Pf1N1B4_2250 0.53 -1.0 2,567,148 + Pf1N1B4_2250 0.53 +0.3 2,567,149 - Pf1N1B4_2250 0.53 +0.2 2,567,149 - Pf1N1B4_2250 0.53 -0.7 2,567,149 - Pf1N1B4_2250 0.53 +0.1 2,567,149 - Pf1N1B4_2250 0.53 +1.7 2,567,199 + Pf1N1B4_2250 0.60 +0.9 2,567,199 + Pf1N1B4_2250 0.60 +1.7 2,567,199 + Pf1N1B4_2250 0.60 -1.7 2,567,200 - Pf1N1B4_2250 0.60 +0.7 2,567,409 + Pf1N1B4_2250 0.87 -0.3 2,567,409 + Pf1N1B4_2250 0.87 -0.7 2,567,409 + Pf1N1B4_2250 0.87 -0.7 2,567,671 + Pf1N1B4_2251 0.20 +0.4 2,567,671 + Pf1N1B4_2251 0.20 -0.1 2,567,672 - Pf1N1B4_2251 0.20 +0.9 2,568,293 - -0.4 2,568,314 + +0.2 2,568,315 - -0.2 2,568,315 - +1.0 2,568,315 - +0.5 2,568,319 + +0.1 2,568,319 + -0.5 2,568,319 + -1.1 2,568,320 - -0.3 2,568,320 - -0.5 2,568,320 - -0.3 2,568,320 - +0.1 2,568,320 - -0.8 2,568,356 + -0.2 2,568,357 - -0.7 2,568,357 - -0.3 2,568,378 - Pf1N1B4_2252 0.12 -1.0 2,568,474 - Pf1N1B4_2252 0.57 -1.8
Or see this region's nucleotide sequence