Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2250

Experiment: L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2249 and Pf1N1B4_2250 are separated by 9 nucleotidesPf1N1B4_2250 and Pf1N1B4_2251 overlap by 4 nucleotidesPf1N1B4_2251 and Pf1N1B4_2252 are separated by 34 nucleotides Pf1N1B4_2249 - Alkanesulfonate monooxygenase (EC 1.14.14.5), at 2,565,573 to 2,566,721 _2249 Pf1N1B4_2250 - Alkanesulfonates transport system permease protein, at 2,566,731 to 2,567,513 _2250 Pf1N1B4_2251 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 2,567,510 to 2,568,316 _2251 Pf1N1B4_2252 - Organosulfonate utilization protein SsuF, at 2,568,351 to 2,568,566 _2252 Position (kb) 2566 2567 2568Strain fitness (log2 ratio) -2 -1 0 1 2at 2566.020 kb on + strand, within Pf1N1B4_2249at 2566.021 kb on - strand, within Pf1N1B4_2249at 2566.063 kb on - strand, within Pf1N1B4_2249at 2566.547 kb on + strand, within Pf1N1B4_2249at 2566.547 kb on + strand, within Pf1N1B4_2249at 2566.547 kb on + strand, within Pf1N1B4_2249at 2566.548 kb on - strand, within Pf1N1B4_2249at 2566.548 kb on - strand, within Pf1N1B4_2249at 2566.606 kb on + strand, within Pf1N1B4_2249at 2566.606 kb on + strand, within Pf1N1B4_2249at 2566.607 kb on - strandat 2566.657 kb on - strandat 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.961 kb on + strand, within Pf1N1B4_2250at 2566.962 kb on - strand, within Pf1N1B4_2250at 2567.136 kb on + strand, within Pf1N1B4_2250at 2567.136 kb on + strand, within Pf1N1B4_2250at 2567.137 kb on - strand, within Pf1N1B4_2250at 2567.137 kb on - strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.148 kb on + strand, within Pf1N1B4_2250at 2567.149 kb on - strand, within Pf1N1B4_2250at 2567.149 kb on - strand, within Pf1N1B4_2250at 2567.149 kb on - strand, within Pf1N1B4_2250at 2567.149 kb on - strand, within Pf1N1B4_2250at 2567.199 kb on + strand, within Pf1N1B4_2250at 2567.199 kb on + strand, within Pf1N1B4_2250at 2567.199 kb on + strand, within Pf1N1B4_2250at 2567.200 kb on - strand, within Pf1N1B4_2250at 2567.409 kb on + strand, within Pf1N1B4_2250at 2567.409 kb on + strand, within Pf1N1B4_2250at 2567.409 kb on + strand, within Pf1N1B4_2250at 2567.671 kb on + strand, within Pf1N1B4_2251at 2567.671 kb on + strand, within Pf1N1B4_2251at 2567.672 kb on - strand, within Pf1N1B4_2251at 2568.293 kb on - strandat 2568.314 kb on + strandat 2568.315 kb on - strandat 2568.315 kb on - strandat 2568.315 kb on - strandat 2568.319 kb on + strandat 2568.319 kb on + strandat 2568.319 kb on + strandat 2568.320 kb on - strandat 2568.320 kb on - strandat 2568.320 kb on - strandat 2568.320 kb on - strandat 2568.320 kb on - strandat 2568.356 kb on + strandat 2568.357 kb on - strandat 2568.357 kb on - strandat 2568.378 kb on - strand, within Pf1N1B4_2252at 2568.474 kb on - strand, within Pf1N1B4_2252

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lactate (C)
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2,566,020 + Pf1N1B4_2249 0.39 -1.4
2,566,021 - Pf1N1B4_2249 0.39 -0.3
2,566,063 - Pf1N1B4_2249 0.43 -1.1
2,566,547 + Pf1N1B4_2249 0.85 +2.4
2,566,547 + Pf1N1B4_2249 0.85 -0.6
2,566,547 + Pf1N1B4_2249 0.85 -0.3
2,566,548 - Pf1N1B4_2249 0.85 -0.5
2,566,548 - Pf1N1B4_2249 0.85 -0.1
2,566,606 + Pf1N1B4_2249 0.90 +1.0
2,566,606 + Pf1N1B4_2249 0.90 -0.5
2,566,607 - -0.4
2,566,657 - -0.1
2,566,961 + Pf1N1B4_2250 0.29 -0.5
2,566,961 + Pf1N1B4_2250 0.29 +0.1
2,566,961 + Pf1N1B4_2250 0.29 -1.3
2,566,961 + Pf1N1B4_2250 0.29 -0.8
2,566,961 + Pf1N1B4_2250 0.29 -0.3
2,566,961 + Pf1N1B4_2250 0.29 +0.9
2,566,961 + Pf1N1B4_2250 0.29 +1.7
2,566,962 - Pf1N1B4_2250 0.30 +0.4
2,567,136 + Pf1N1B4_2250 0.52 +0.4
2,567,136 + Pf1N1B4_2250 0.52 -1.7
2,567,137 - Pf1N1B4_2250 0.52 +0.1
2,567,137 - Pf1N1B4_2250 0.52 +0.3
2,567,148 + Pf1N1B4_2250 0.53 -0.7
2,567,148 + Pf1N1B4_2250 0.53 +0.3
2,567,148 + Pf1N1B4_2250 0.53 +0.1
2,567,148 + Pf1N1B4_2250 0.53 +1.0
2,567,148 + Pf1N1B4_2250 0.53 +1.4
2,567,148 + Pf1N1B4_2250 0.53 -1.0
2,567,148 + Pf1N1B4_2250 0.53 +0.3
2,567,149 - Pf1N1B4_2250 0.53 +0.2
2,567,149 - Pf1N1B4_2250 0.53 -0.7
2,567,149 - Pf1N1B4_2250 0.53 +0.1
2,567,149 - Pf1N1B4_2250 0.53 +1.7
2,567,199 + Pf1N1B4_2250 0.60 +0.9
2,567,199 + Pf1N1B4_2250 0.60 +1.7
2,567,199 + Pf1N1B4_2250 0.60 -1.7
2,567,200 - Pf1N1B4_2250 0.60 +0.7
2,567,409 + Pf1N1B4_2250 0.87 -0.3
2,567,409 + Pf1N1B4_2250 0.87 -0.7
2,567,409 + Pf1N1B4_2250 0.87 -0.7
2,567,671 + Pf1N1B4_2251 0.20 +0.4
2,567,671 + Pf1N1B4_2251 0.20 -0.1
2,567,672 - Pf1N1B4_2251 0.20 +0.9
2,568,293 - -0.4
2,568,314 + +0.2
2,568,315 - -0.2
2,568,315 - +1.0
2,568,315 - +0.5
2,568,319 + +0.1
2,568,319 + -0.5
2,568,319 + -1.1
2,568,320 - -0.3
2,568,320 - -0.5
2,568,320 - -0.3
2,568,320 - +0.1
2,568,320 - -0.8
2,568,356 + -0.2
2,568,357 - -0.7
2,568,357 - -0.3
2,568,378 - Pf1N1B4_2252 0.12 -1.0
2,568,474 - Pf1N1B4_2252 0.57 -1.8

Or see this region's nucleotide sequence