Experiment: R2A with Zinc 0.08 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22540 and CA265_RS22545 are separated by 86 nucleotides CA265_RS22545 and CA265_RS22550 are separated by 33 nucleotides CA265_RS22550 and CA265_RS22555 overlap by 1 nucleotides CA265_RS22555 and CA265_RS22560 are separated by 11 nucleotides
CA265_RS22540: CA265_RS22540 - hypothetical protein, at 5,524,638 to 5,525,126
_RS22540
CA265_RS22545: CA265_RS22545 - lysophospholipase, at 5,525,213 to 5,525,938
_RS22545
CA265_RS22550: CA265_RS22550 - hypothetical protein, at 5,525,972 to 5,527,030
_RS22550
CA265_RS22555: CA265_RS22555 - lipoyl(octanoyl) transferase, at 5,527,030 to 5,527,758
_RS22555
CA265_RS22560: CA265_RS22560 - 4'-phosphopantetheinyl transferase, at 5,527,770 to 5,528,417
_RS22560
Position (kb) 5525
5526
5527
5528 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5524.983 kb on + strand, within CA265_RS22540 at 5524.984 kb on - strand at 5524.985 kb on + strand, within CA265_RS22540 at 5524.985 kb on + strand, within CA265_RS22540 at 5525.040 kb on + strand, within CA265_RS22540 at 5525.041 kb on - strand, within CA265_RS22540 at 5525.049 kb on + strand, within CA265_RS22540 at 5525.132 kb on - strand at 5525.159 kb on + strand at 5525.241 kb on - strand at 5525.304 kb on - strand, within CA265_RS22545 at 5525.341 kb on + strand, within CA265_RS22545 at 5525.341 kb on + strand, within CA265_RS22545 at 5525.347 kb on + strand, within CA265_RS22545 at 5525.413 kb on + strand, within CA265_RS22545 at 5525.483 kb on + strand, within CA265_RS22545 at 5525.494 kb on + strand, within CA265_RS22545 at 5525.532 kb on - strand, within CA265_RS22545 at 5525.600 kb on + strand, within CA265_RS22545 at 5525.608 kb on + strand, within CA265_RS22545 at 5525.608 kb on + strand, within CA265_RS22545 at 5525.608 kb on + strand, within CA265_RS22545 at 5525.658 kb on - strand, within CA265_RS22545 at 5525.787 kb on + strand, within CA265_RS22545 at 5525.808 kb on + strand, within CA265_RS22545 at 5525.812 kb on + strand, within CA265_RS22545 at 5525.813 kb on - strand, within CA265_RS22545 at 5525.912 kb on + strand at 5525.939 kb on - strand at 5525.943 kb on + strand at 5526.023 kb on - strand at 5526.105 kb on - strand, within CA265_RS22550 at 5526.152 kb on - strand, within CA265_RS22550 at 5526.152 kb on - strand, within CA265_RS22550 at 5526.187 kb on + strand, within CA265_RS22550 at 5526.218 kb on - strand, within CA265_RS22550 at 5526.294 kb on - strand, within CA265_RS22550 at 5526.313 kb on + strand, within CA265_RS22550 at 5526.322 kb on + strand, within CA265_RS22550 at 5526.550 kb on + strand, within CA265_RS22550 at 5526.551 kb on - strand, within CA265_RS22550 at 5526.577 kb on + strand, within CA265_RS22550 at 5526.618 kb on + strand, within CA265_RS22550 at 5526.619 kb on - strand, within CA265_RS22550 at 5526.640 kb on + strand, within CA265_RS22550 at 5526.641 kb on - strand, within CA265_RS22550 at 5526.721 kb on + strand, within CA265_RS22550 at 5526.722 kb on - strand, within CA265_RS22550 at 5526.751 kb on - strand, within CA265_RS22550 at 5526.753 kb on + strand, within CA265_RS22550 at 5526.778 kb on + strand, within CA265_RS22550 at 5526.779 kb on - strand, within CA265_RS22550 at 5526.882 kb on + strand, within CA265_RS22550 at 5526.883 kb on - strand, within CA265_RS22550 at 5526.890 kb on + strand, within CA265_RS22550 at 5526.914 kb on + strand, within CA265_RS22550 at 5526.915 kb on - strand, within CA265_RS22550 at 5526.991 kb on + strand at 5526.991 kb on + strand at 5526.991 kb on + strand at 5526.991 kb on + strand at 5527.002 kb on - strand at 5527.031 kb on + strand at 5527.767 kb on - strand at 5527.785 kb on + strand at 5527.785 kb on + strand at 5527.795 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc 0.08 mM remove 5,524,983 + CA265_RS22540 0.71 -0.3 5,524,984 - +4.0 5,524,985 + CA265_RS22540 0.71 +1.1 5,524,985 + CA265_RS22540 0.71 +2.0 5,525,040 + CA265_RS22540 0.82 +0.3 5,525,041 - CA265_RS22540 0.82 -0.5 5,525,049 + CA265_RS22540 0.84 -1.0 5,525,132 - -1.0 5,525,159 + -0.5 5,525,241 - -0.6 5,525,304 - CA265_RS22545 0.13 -0.2 5,525,341 + CA265_RS22545 0.18 -0.6 5,525,341 + CA265_RS22545 0.18 +0.4 5,525,347 + CA265_RS22545 0.18 +0.4 5,525,413 + CA265_RS22545 0.28 -0.7 5,525,483 + CA265_RS22545 0.37 -0.6 5,525,494 + CA265_RS22545 0.39 -0.6 5,525,532 - CA265_RS22545 0.44 +0.0 5,525,600 + CA265_RS22545 0.53 +0.2 5,525,608 + CA265_RS22545 0.54 +0.5 5,525,608 + CA265_RS22545 0.54 -0.1 5,525,608 + CA265_RS22545 0.54 -1.4 5,525,658 - CA265_RS22545 0.61 -1.9 5,525,787 + CA265_RS22545 0.79 -1.3 5,525,808 + CA265_RS22545 0.82 -0.6 5,525,812 + CA265_RS22545 0.83 +0.4 5,525,813 - CA265_RS22545 0.83 +1.2 5,525,912 + +0.3 5,525,939 - -0.8 5,525,943 + +0.2 5,526,023 - -1.1 5,526,105 - CA265_RS22550 0.13 +0.0 5,526,152 - CA265_RS22550 0.17 -0.1 5,526,152 - CA265_RS22550 0.17 -1.0 5,526,187 + CA265_RS22550 0.20 -0.5 5,526,218 - CA265_RS22550 0.23 -0.6 5,526,294 - CA265_RS22550 0.30 +0.3 5,526,313 + CA265_RS22550 0.32 +0.7 5,526,322 + CA265_RS22550 0.33 -2.3 5,526,550 + CA265_RS22550 0.55 +0.6 5,526,551 - CA265_RS22550 0.55 +0.2 5,526,577 + CA265_RS22550 0.57 +0.1 5,526,618 + CA265_RS22550 0.61 +0.6 5,526,619 - CA265_RS22550 0.61 -1.1 5,526,640 + CA265_RS22550 0.63 +0.2 5,526,641 - CA265_RS22550 0.63 +0.4 5,526,721 + CA265_RS22550 0.71 +0.1 5,526,722 - CA265_RS22550 0.71 -1.0 5,526,751 - CA265_RS22550 0.74 +0.4 5,526,753 + CA265_RS22550 0.74 +0.8 5,526,778 + CA265_RS22550 0.76 -0.1 5,526,779 - CA265_RS22550 0.76 -0.2 5,526,882 + CA265_RS22550 0.86 +0.2 5,526,883 - CA265_RS22550 0.86 +0.2 5,526,890 + CA265_RS22550 0.87 +0.4 5,526,914 + CA265_RS22550 0.89 +0.4 5,526,915 - CA265_RS22550 0.89 -0.3 5,526,991 + +0.4 5,526,991 + +1.2 5,526,991 + +0.6 5,526,991 + +0.5 5,527,002 - +1.6 5,527,031 + +0.7 5,527,767 - +0.5 5,527,785 + +1.0 5,527,785 + -0.7 5,527,795 - -1.8
Or see this region's nucleotide sequence