Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31300 and BPHYT_RS31305 are separated by 81 nucleotides BPHYT_RS31305 and BPHYT_RS31310 are separated by 94 nucleotides BPHYT_RS31310 and BPHYT_RS31315 are separated by 245 nucleotides
BPHYT_RS31300: BPHYT_RS31300 - Crp/Fnr family transcriptional regulator, at 2,581,378 to 2,582,166
_RS31300
BPHYT_RS31305: BPHYT_RS31305 - alcohol dehydrogenase, at 2,582,248 to 2,583,234
_RS31305
BPHYT_RS31310: BPHYT_RS31310 - hypothetical protein, at 2,583,329 to 2,583,604
_RS31310
BPHYT_RS31315: BPHYT_RS31315 - cytochrome C, at 2,583,850 to 2,584,185
_RS31315
Position (kb)
2582
2583
2584 Strain fitness (log2 ratio)
-2
-1
0
1 at 2581.313 kb on + strand at 2581.519 kb on - strand, within BPHYT_RS31300 at 2581.643 kb on - strand, within BPHYT_RS31300 at 2581.765 kb on - strand, within BPHYT_RS31300 at 2582.677 kb on - strand, within BPHYT_RS31305 at 2582.732 kb on + strand, within BPHYT_RS31305 at 2583.221 kb on - strand at 2583.734 kb on - strand at 2584.195 kb on + strand at 2584.220 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 2,581,313 + -0.2 2,581,519 - BPHYT_RS31300 0.18 -0.8 2,581,643 - BPHYT_RS31300 0.34 -0.1 2,581,765 - BPHYT_RS31300 0.49 -0.1 2,582,677 - BPHYT_RS31305 0.43 -0.1 2,582,732 + BPHYT_RS31305 0.49 +0.5 2,583,221 - -0.3 2,583,734 - +0.5 2,584,195 + +0.3 2,584,220 + -2.5
Or see this region's nucleotide sequence