Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30140 and BPHYT_RS30145 are separated by 110 nucleotides BPHYT_RS30145 and BPHYT_RS30150 are separated by 328 nucleotides
BPHYT_RS30140: BPHYT_RS30140 - 3-methylitaconate isomerase, at 2,313,771 to 2,314,958
_RS30140
BPHYT_RS30145: BPHYT_RS30145 - aconitate hydratase, at 2,315,069 to 2,317,666
_RS30145
BPHYT_RS30150: BPHYT_RS30150 - methylcitrate synthase, at 2,317,995 to 2,319,164
_RS30150
Position (kb)
2315
2316
2317
2318 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2314.921 kb on + strand at 2314.921 kb on + strand at 2314.922 kb on - strand at 2314.922 kb on - strand at 2314.989 kb on - strand at 2315.488 kb on - strand, within BPHYT_RS30145 at 2315.844 kb on + strand, within BPHYT_RS30145 at 2315.844 kb on + strand, within BPHYT_RS30145 at 2315.845 kb on - strand, within BPHYT_RS30145 at 2315.845 kb on - strand, within BPHYT_RS30145 at 2315.922 kb on + strand, within BPHYT_RS30145 at 2316.973 kb on + strand, within BPHYT_RS30145 at 2316.975 kb on + strand, within BPHYT_RS30145 at 2316.975 kb on + strand, within BPHYT_RS30145 at 2317.493 kb on - strand at 2317.560 kb on + strand at 2317.661 kb on - strand at 2317.687 kb on - strand at 2317.736 kb on + strand at 2317.739 kb on - strand at 2317.741 kb on - strand at 2317.741 kb on - strand at 2317.770 kb on + strand at 2317.770 kb on + strand at 2317.781 kb on + strand at 2317.899 kb on - strand at 2318.045 kb on - strand at 2318.170 kb on + strand, within BPHYT_RS30150 at 2318.336 kb on - strand, within BPHYT_RS30150 at 2318.506 kb on + strand, within BPHYT_RS30150 at 2318.510 kb on + strand, within BPHYT_RS30150
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 2,314,921 + +1.4 2,314,921 + -0.7 2,314,922 - -0.7 2,314,922 - +0.6 2,314,989 - +1.6 2,315,488 - BPHYT_RS30145 0.16 +0.3 2,315,844 + BPHYT_RS30145 0.30 +0.5 2,315,844 + BPHYT_RS30145 0.30 +0.3 2,315,845 - BPHYT_RS30145 0.30 +0.1 2,315,845 - BPHYT_RS30145 0.30 -0.1 2,315,922 + BPHYT_RS30145 0.33 -2.3 2,316,973 + BPHYT_RS30145 0.73 +0.6 2,316,975 + BPHYT_RS30145 0.73 -0.3 2,316,975 + BPHYT_RS30145 0.73 +0.9 2,317,493 - +1.8 2,317,560 + +1.0 2,317,661 - +1.0 2,317,687 - -2.0 2,317,736 + +0.0 2,317,739 - -0.5 2,317,741 - +0.3 2,317,741 - +0.2 2,317,770 + +0.0 2,317,770 + -1.9 2,317,781 + -0.3 2,317,899 - +0.4 2,318,045 - +0.5 2,318,170 + BPHYT_RS30150 0.15 -3.0 2,318,336 - BPHYT_RS30150 0.29 +0.4 2,318,506 + BPHYT_RS30150 0.44 -0.4 2,318,510 + BPHYT_RS30150 0.44 -0.3
Or see this region's nucleotide sequence