Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27765 and BPHYT_RS27770 are separated by 64 nucleotides BPHYT_RS27770 and BPHYT_RS27775 are separated by 198 nucleotides BPHYT_RS27775 and BPHYT_RS27780 are separated by 15 nucleotides BPHYT_RS27780 and BPHYT_RS27785 are separated by 102 nucleotides BPHYT_RS27785 and BPHYT_RS27790 are separated by 85 nucleotides
BPHYT_RS27765: BPHYT_RS27765 - ABC transporter permease, at 1,790,899 to 1,792,035
_RS27765
BPHYT_RS27770: BPHYT_RS27770 - hypothetical protein, at 1,792,100 to 1,792,291
_RS27770
BPHYT_RS27775: BPHYT_RS27775 - hypothetical protein, at 1,792,490 to 1,792,672
_RS27775
BPHYT_RS27780: BPHYT_RS27780 - acetyl-CoA synthetase, at 1,792,688 to 1,794,664
_RS27780
BPHYT_RS27785: BPHYT_RS27785 - hypothetical protein, at 1,794,767 to 1,795,372
_RS27785
BPHYT_RS27790: BPHYT_RS27790 - poly-beta-hydroxybutyrate polymerase, at 1,795,458 to 1,797,314
_RS27790
Position (kb)
1792
1793
1794
1795 Strain fitness (log2 ratio)
-1
0
1 at 1791.713 kb on + strand, within BPHYT_RS27765 at 1792.872 kb on + strand at 1792.898 kb on + strand, within BPHYT_RS27780 at 1793.642 kb on + strand, within BPHYT_RS27780 at 1793.643 kb on - strand, within BPHYT_RS27780 at 1793.643 kb on - strand, within BPHYT_RS27780 at 1793.763 kb on - strand, within BPHYT_RS27780 at 1795.253 kb on + strand, within BPHYT_RS27785 at 1795.359 kb on + strand at 1795.446 kb on + strand at 1795.447 kb on - strand at 1795.657 kb on + strand, within BPHYT_RS27790 at 1795.657 kb on + strand, within BPHYT_RS27790
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 1,791,713 + BPHYT_RS27765 0.72 +0.0 1,792,872 + -0.8 1,792,898 + BPHYT_RS27780 0.11 -1.3 1,793,642 + BPHYT_RS27780 0.48 -0.8 1,793,643 - BPHYT_RS27780 0.48 -0.3 1,793,643 - BPHYT_RS27780 0.48 +0.3 1,793,763 - BPHYT_RS27780 0.54 -0.4 1,795,253 + BPHYT_RS27785 0.80 +1.2 1,795,359 + -1.1 1,795,446 + -1.6 1,795,447 - -0.7 1,795,657 + BPHYT_RS27790 0.11 -0.2 1,795,657 + BPHYT_RS27790 0.11 -0.3
Or see this region's nucleotide sequence