Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20930 and BPHYT_RS20935 are separated by 37 nucleotides BPHYT_RS20935 and BPHYT_RS20940 are separated by 146 nucleotides BPHYT_RS20940 and BPHYT_RS20945 are separated by 95 nucleotides BPHYT_RS20945 and BPHYT_RS20950 are separated by 25 nucleotides
BPHYT_RS20930: BPHYT_RS20930 - hypothetical protein, at 257,654 to 257,941
_RS20930
BPHYT_RS20935: BPHYT_RS20935 - dehydrogenase, at 257,979 to 258,413
_RS20935
BPHYT_RS20940: BPHYT_RS20940 - citrate synthase, at 258,560 to 259,804
_RS20940
BPHYT_RS20945: BPHYT_RS20945 - glyoxalase, at 259,900 to 260,289
_RS20945
BPHYT_RS20950: BPHYT_RS20950 - D-galactonate transporter, at 260,315 to 261,631
_RS20950
Position (kb)
258
259
260 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 257.596 kb on + strand at 257.596 kb on + strand at 257.596 kb on + strand at 257.599 kb on + strand at 257.648 kb on + strand at 257.649 kb on - strand at 257.649 kb on - strand at 257.649 kb on - strand at 257.664 kb on - strand at 257.715 kb on - strand, within BPHYT_RS20930 at 257.715 kb on - strand, within BPHYT_RS20930 at 257.981 kb on - strand at 257.988 kb on - strand at 257.988 kb on - strand at 258.037 kb on - strand, within BPHYT_RS20935 at 258.047 kb on + strand, within BPHYT_RS20935 at 258.048 kb on - strand, within BPHYT_RS20935 at 258.275 kb on + strand, within BPHYT_RS20935 at 259.534 kb on - strand, within BPHYT_RS20940 at 259.572 kb on + strand, within BPHYT_RS20940 at 259.596 kb on - strand, within BPHYT_RS20940 at 259.734 kb on + strand at 259.735 kb on - strand at 259.735 kb on - strand at 259.735 kb on - strand at 259.740 kb on + strand at 259.741 kb on - strand at 260.289 kb on + strand at 260.290 kb on - strand at 260.379 kb on - strand at 260.509 kb on - strand, within BPHYT_RS20950 at 260.797 kb on + strand, within BPHYT_RS20950 at 260.798 kb on - strand, within BPHYT_RS20950
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 257,596 + +0.4 257,596 + +1.8 257,596 + -0.1 257,599 + -0.3 257,648 + +0.6 257,649 - -0.5 257,649 - -0.2 257,649 - -0.2 257,664 - +0.0 257,715 - BPHYT_RS20930 0.21 -0.6 257,715 - BPHYT_RS20930 0.21 +1.0 257,981 - -2.2 257,988 - -0.4 257,988 - +0.6 258,037 - BPHYT_RS20935 0.13 +0.6 258,047 + BPHYT_RS20935 0.16 -2.8 258,048 - BPHYT_RS20935 0.16 -2.2 258,275 + BPHYT_RS20935 0.68 -0.3 259,534 - BPHYT_RS20940 0.78 -1.2 259,572 + BPHYT_RS20940 0.81 +0.5 259,596 - BPHYT_RS20940 0.83 -0.1 259,734 + -0.2 259,735 - -0.6 259,735 - +0.6 259,735 - +0.3 259,740 + -0.3 259,741 - -0.1 260,289 + -1.1 260,290 - -0.4 260,379 - -1.4 260,509 - BPHYT_RS20950 0.15 -0.4 260,797 + BPHYT_RS20950 0.37 +0.1 260,798 - BPHYT_RS20950 0.37 +0.7
Or see this region's nucleotide sequence