Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18330 and BPHYT_RS18335 are separated by 216 nucleotides BPHYT_RS18335 and BPHYT_RS18340 are separated by 184 nucleotides BPHYT_RS18340 and BPHYT_RS18345 are separated by 494 nucleotides
BPHYT_RS18330: BPHYT_RS18330 - phosphatidylethanolamine-binding protein, at 4,123,985 to 4,124,587
_RS18330
BPHYT_RS18335: BPHYT_RS18335 - NADPH-dependent FMN reductase, at 4,124,804 to 4,125,376
_RS18335
BPHYT_RS18340: BPHYT_RS18340 - n-acetyl-gamma-glutamyl-phosphate reductase, at 4,125,561 to 4,126,508
_RS18340
BPHYT_RS18345: BPHYT_RS18345 - hypothetical protein, at 4,127,003 to 4,128,433
_RS18345
Position (kb) 4125
4126
4127 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4124.577 kb on - strand at 4124.585 kb on + strand at 4124.586 kb on - strand at 4124.586 kb on - strand at 4124.657 kb on + strand at 4124.658 kb on - strand at 4124.696 kb on + strand at 4124.697 kb on - strand at 4124.826 kb on - strand at 4124.933 kb on - strand, within BPHYT_RS18335 at 4124.987 kb on + strand, within BPHYT_RS18335 at 4124.988 kb on - strand, within BPHYT_RS18335 at 4124.988 kb on - strand, within BPHYT_RS18335 at 4124.988 kb on - strand, within BPHYT_RS18335 at 4125.227 kb on + strand, within BPHYT_RS18335 at 4125.228 kb on - strand, within BPHYT_RS18335 at 4125.228 kb on - strand, within BPHYT_RS18335 at 4125.228 kb on - strand, within BPHYT_RS18335 at 4125.228 kb on - strand, within BPHYT_RS18335 at 4125.228 kb on - strand, within BPHYT_RS18335 at 4125.228 kb on - strand, within BPHYT_RS18335 at 4125.228 kb on - strand, within BPHYT_RS18335 at 4125.228 kb on - strand, within BPHYT_RS18335 at 4125.336 kb on + strand at 4126.098 kb on - strand, within BPHYT_RS18340 at 4126.266 kb on + strand, within BPHYT_RS18340 at 4126.631 kb on - strand at 4126.666 kb on + strand at 4126.771 kb on + strand at 4126.982 kb on + strand at 4126.983 kb on - strand at 4127.227 kb on + strand, within BPHYT_RS18345 at 4127.273 kb on + strand, within BPHYT_RS18345 at 4127.273 kb on + strand, within BPHYT_RS18345 at 4127.274 kb on - strand, within BPHYT_RS18345 at 4127.274 kb on - strand, within BPHYT_RS18345
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 4,124,577 - +0.7 4,124,585 + +0.4 4,124,586 - -0.6 4,124,586 - -0.3 4,124,657 + -0.7 4,124,658 - +1.2 4,124,696 + +1.2 4,124,697 - +0.5 4,124,826 - +0.6 4,124,933 - BPHYT_RS18335 0.23 -1.4 4,124,987 + BPHYT_RS18335 0.32 -0.0 4,124,988 - BPHYT_RS18335 0.32 -1.8 4,124,988 - BPHYT_RS18335 0.32 -0.5 4,124,988 - BPHYT_RS18335 0.32 +0.2 4,125,227 + BPHYT_RS18335 0.74 +0.2 4,125,228 - BPHYT_RS18335 0.74 +0.3 4,125,228 - BPHYT_RS18335 0.74 +0.2 4,125,228 - BPHYT_RS18335 0.74 -0.5 4,125,228 - BPHYT_RS18335 0.74 +0.7 4,125,228 - BPHYT_RS18335 0.74 +0.4 4,125,228 - BPHYT_RS18335 0.74 -0.1 4,125,228 - BPHYT_RS18335 0.74 -0.4 4,125,228 - BPHYT_RS18335 0.74 -0.5 4,125,336 + -0.2 4,126,098 - BPHYT_RS18340 0.57 -0.3 4,126,266 + BPHYT_RS18340 0.74 -1.3 4,126,631 - +0.7 4,126,666 + -3.5 4,126,771 + -0.3 4,126,982 + -1.4 4,126,983 - -0.1 4,127,227 + BPHYT_RS18345 0.16 +0.7 4,127,273 + BPHYT_RS18345 0.19 +2.2 4,127,273 + BPHYT_RS18345 0.19 +0.3 4,127,274 - BPHYT_RS18345 0.19 -0.2 4,127,274 - BPHYT_RS18345 0.19 -0.2
Or see this region's nucleotide sequence