Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00195 and BPHYT_RS00200 are separated by 800 nucleotides BPHYT_RS00200 and BPHYT_RS00205 are separated by 185 nucleotides BPHYT_RS00205 and BPHYT_RS00210 overlap by 23 nucleotides
BPHYT_RS00195: BPHYT_RS00195 - LysR family transcriptional regulator, at 51,437 to 52,348
_RS00195
BPHYT_RS00200: BPHYT_RS00200 - amidase, at 53,149 to 54,552
_RS00200
BPHYT_RS00205: BPHYT_RS00205 - DeoR family transcriptional regulator, at 54,738 to 55,496
_RS00205
BPHYT_RS00210: BPHYT_RS00210 - allantoate amidohydrolase, at 55,474 to 56,766
_RS00210
Position (kb) 53
54
55 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 52.250 kb on + strand, within BPHYT_RS00195 at 52.253 kb on + strand, within BPHYT_RS00195 at 52.253 kb on + strand, within BPHYT_RS00195 at 52.254 kb on - strand, within BPHYT_RS00195 at 52.254 kb on - strand, within BPHYT_RS00195 at 52.254 kb on - strand, within BPHYT_RS00195 at 52.501 kb on - strand at 52.597 kb on - strand at 52.616 kb on - strand at 52.729 kb on + strand at 52.729 kb on + strand at 52.759 kb on + strand at 52.759 kb on + strand at 52.983 kb on - strand at 52.993 kb on + strand at 52.993 kb on + strand at 53.276 kb on - strand at 53.322 kb on + strand, within BPHYT_RS00200 at 53.323 kb on - strand, within BPHYT_RS00200 at 53.424 kb on + strand, within BPHYT_RS00200 at 53.536 kb on + strand, within BPHYT_RS00200 at 53.537 kb on - strand, within BPHYT_RS00200 at 53.554 kb on - strand, within BPHYT_RS00200 at 53.699 kb on + strand, within BPHYT_RS00200 at 53.699 kb on + strand, within BPHYT_RS00200 at 53.699 kb on + strand, within BPHYT_RS00200 at 53.792 kb on + strand, within BPHYT_RS00200 at 53.875 kb on + strand, within BPHYT_RS00200 at 53.876 kb on - strand, within BPHYT_RS00200 at 54.094 kb on + strand, within BPHYT_RS00200 at 54.152 kb on + strand, within BPHYT_RS00200 at 54.152 kb on + strand, within BPHYT_RS00200 at 54.248 kb on - strand, within BPHYT_RS00200 at 54.566 kb on - strand at 54.566 kb on - strand at 54.566 kb on - strand at 54.671 kb on + strand at 54.708 kb on - strand at 54.715 kb on + strand at 54.756 kb on - strand at 55.202 kb on + strand, within BPHYT_RS00205 at 55.231 kb on - strand, within BPHYT_RS00205 at 55.450 kb on - strand at 55.460 kb on + strand at 55.483 kb on + strand at 55.483 kb on + strand at 55.484 kb on - strand at 55.484 kb on - strand at 55.484 kb on - strand at 55.494 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 52,250 + BPHYT_RS00195 0.89 -0.8 52,253 + BPHYT_RS00195 0.89 +0.3 52,253 + BPHYT_RS00195 0.89 -0.4 52,254 - BPHYT_RS00195 0.90 +0.1 52,254 - BPHYT_RS00195 0.90 -3.2 52,254 - BPHYT_RS00195 0.90 -2.1 52,501 - +3.5 52,597 - +0.8 52,616 - +0.1 52,729 + -2.9 52,729 + +0.2 52,759 + -1.9 52,759 + +0.1 52,983 - +1.0 52,993 + -0.4 52,993 + -2.4 53,276 - -0.2 53,322 + BPHYT_RS00200 0.12 -1.4 53,323 - BPHYT_RS00200 0.12 +0.1 53,424 + BPHYT_RS00200 0.20 -0.5 53,536 + BPHYT_RS00200 0.28 -0.1 53,537 - BPHYT_RS00200 0.28 -0.3 53,554 - BPHYT_RS00200 0.29 -0.6 53,699 + BPHYT_RS00200 0.39 -0.6 53,699 + BPHYT_RS00200 0.39 +1.8 53,699 + BPHYT_RS00200 0.39 -0.0 53,792 + BPHYT_RS00200 0.46 +0.2 53,875 + BPHYT_RS00200 0.52 -0.2 53,876 - BPHYT_RS00200 0.52 -0.2 54,094 + BPHYT_RS00200 0.67 +0.3 54,152 + BPHYT_RS00200 0.71 -2.5 54,152 + BPHYT_RS00200 0.71 +0.3 54,248 - BPHYT_RS00200 0.78 -0.2 54,566 - -2.4 54,566 - -0.0 54,566 - -1.0 54,671 + -0.5 54,708 - -0.6 54,715 + +0.6 54,756 - +0.1 55,202 + BPHYT_RS00205 0.61 -0.5 55,231 - BPHYT_RS00205 0.65 -0.7 55,450 - -0.5 55,460 + -1.4 55,483 + -2.3 55,483 + +1.3 55,484 - +0.5 55,484 - -0.2 55,484 - -2.7 55,494 + -1.9
Or see this region's nucleotide sequence