Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_70

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_69 and Pf1N1B4_70 are separated by 388 nucleotidesPf1N1B4_70 and Pf1N1B4_71 are separated by 23 nucleotides Pf1N1B4_69 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 71,829 to 72,542 _69 Pf1N1B4_70 - Major facilitator family transporter, at 72,931 to 74,256 _70 Pf1N1B4_71 - Glucarate dehydratase (EC 4.2.1.40), at 74,280 to 75,623 _71 Position (kb) 72 73 74 75Strain fitness (log2 ratio) -2 -1 0 1 2 3at 72.028 kb on - strand, within Pf1N1B4_69at 72.028 kb on - strand, within Pf1N1B4_69at 72.139 kb on + strand, within Pf1N1B4_69at 72.140 kb on - strand, within Pf1N1B4_69at 72.303 kb on + strand, within Pf1N1B4_69at 72.304 kb on - strand, within Pf1N1B4_69at 72.577 kb on - strandat 72.648 kb on - strandat 72.749 kb on - strandat 72.915 kb on + strandat 72.916 kb on - strandat 72.916 kb on - strandat 72.916 kb on - strandat 73.024 kb on + strandat 73.024 kb on + strandat 73.024 kb on + strandat 73.024 kb on + strandat 73.024 kb on + strandat 73.024 kb on + strandat 73.025 kb on - strandat 73.025 kb on - strandat 73.262 kb on - strand, within Pf1N1B4_70at 73.318 kb on + strandat 73.319 kb on - strand, within Pf1N1B4_70at 73.319 kb on - strand, within Pf1N1B4_70at 73.321 kb on + strand, within Pf1N1B4_70at 73.321 kb on + strand, within Pf1N1B4_70at 73.322 kb on - strand, within Pf1N1B4_70at 73.392 kb on - strandat 73.471 kb on + strand, within Pf1N1B4_70at 73.471 kb on + strandat 73.471 kb on + strand, within Pf1N1B4_70at 73.472 kb on - strand, within Pf1N1B4_70at 73.472 kb on - strand, within Pf1N1B4_70at 73.472 kb on - strandat 73.690 kb on + strand, within Pf1N1B4_70at 73.690 kb on + strand, within Pf1N1B4_70at 73.690 kb on + strand, within Pf1N1B4_70at 73.690 kb on + strand, within Pf1N1B4_70at 73.691 kb on - strand, within Pf1N1B4_70at 73.691 kb on - strand, within Pf1N1B4_70at 73.715 kb on - strand, within Pf1N1B4_70at 73.825 kb on + strand, within Pf1N1B4_70at 73.825 kb on + strand, within Pf1N1B4_70at 73.825 kb on + strand, within Pf1N1B4_70at 73.825 kb on + strand, within Pf1N1B4_70at 73.826 kb on - strand, within Pf1N1B4_70at 73.826 kb on - strand, within Pf1N1B4_70at 73.826 kb on - strand, within Pf1N1B4_70at 73.826 kb on - strand, within Pf1N1B4_70at 74.015 kb on - strand, within Pf1N1B4_70at 74.244 kb on + strandat 74.245 kb on - strandat 74.245 kb on - strandat 74.245 kb on - strandat 74.245 kb on - strandat 74.245 kb on - strandat 74.245 kb on - strandat 74.268 kb on - strandat 74.465 kb on + strand, within Pf1N1B4_71at 74.523 kb on + strand, within Pf1N1B4_71at 74.742 kb on + strand, within Pf1N1B4_71at 74.742 kb on + strand, within Pf1N1B4_71at 74.742 kb on + strand, within Pf1N1B4_71at 74.742 kb on + strand, within Pf1N1B4_71at 74.743 kb on - strand, within Pf1N1B4_71at 74.743 kb on - strand, within Pf1N1B4_71at 75.206 kb on - strand, within Pf1N1B4_71at 75.210 kb on - strand, within Pf1N1B4_71

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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72,028 - Pf1N1B4_69 0.28 -1.1
72,028 - Pf1N1B4_69 0.28 -0.5
72,139 + Pf1N1B4_69 0.43 +0.3
72,140 - Pf1N1B4_69 0.44 -0.7
72,303 + Pf1N1B4_69 0.66 -0.1
72,304 - Pf1N1B4_69 0.67 +0.5
72,577 - +0.0
72,648 - -1.7
72,749 - -2.2
72,915 + +1.9
72,916 - -0.7
72,916 - +0.2
72,916 - -0.1
73,024 + +0.3
73,024 + +1.0
73,024 + +0.1
73,024 + -0.1
73,024 + -0.1
73,024 + +1.9
73,025 - +0.9
73,025 - +0.8
73,262 - Pf1N1B4_70 0.25 +0.5
73,318 + +0.3
73,319 - Pf1N1B4_70 0.29 +0.2
73,319 - Pf1N1B4_70 0.29 +0.9
73,321 + Pf1N1B4_70 0.29 +0.5
73,321 + Pf1N1B4_70 0.29 -0.5
73,322 - Pf1N1B4_70 0.29 +0.9
73,392 - +0.3
73,471 + Pf1N1B4_70 0.41 -0.1
73,471 + +0.5
73,471 + Pf1N1B4_70 0.41 +0.0
73,472 - Pf1N1B4_70 0.41 +0.1
73,472 - Pf1N1B4_70 0.41 +1.1
73,472 - +2.5
73,690 + Pf1N1B4_70 0.57 +0.9
73,690 + Pf1N1B4_70 0.57 -0.5
73,690 + Pf1N1B4_70 0.57 -0.0
73,690 + Pf1N1B4_70 0.57 -2.2
73,691 - Pf1N1B4_70 0.57 -0.1
73,691 - Pf1N1B4_70 0.57 -0.7
73,715 - Pf1N1B4_70 0.59 -0.1
73,825 + Pf1N1B4_70 0.67 -0.5
73,825 + Pf1N1B4_70 0.67 +0.7
73,825 + Pf1N1B4_70 0.67 -0.7
73,825 + Pf1N1B4_70 0.67 +3.2
73,826 - Pf1N1B4_70 0.67 -1.7
73,826 - Pf1N1B4_70 0.67 +1.3
73,826 - Pf1N1B4_70 0.67 +0.9
73,826 - Pf1N1B4_70 0.67 +1.7
74,015 - Pf1N1B4_70 0.82 -0.7
74,244 + +0.9
74,245 - +0.4
74,245 - -2.2
74,245 - -0.1
74,245 - -0.9
74,245 - -0.1
74,245 - -0.2
74,268 - +0.9
74,465 + Pf1N1B4_71 0.14 +0.2
74,523 + Pf1N1B4_71 0.18 +0.8
74,742 + Pf1N1B4_71 0.34 +0.5
74,742 + Pf1N1B4_71 0.34 -0.1
74,742 + Pf1N1B4_71 0.34 -0.2
74,742 + Pf1N1B4_71 0.34 -0.9
74,743 - Pf1N1B4_71 0.34 +0.5
74,743 - Pf1N1B4_71 0.34 +0.3
75,206 - Pf1N1B4_71 0.69 +0.7
75,210 - Pf1N1B4_71 0.69 -0.9

Or see this region's nucleotide sequence