Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_67

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_65 and Pf1N1B4_66 are separated by 61 nucleotidesPf1N1B4_66 and Pf1N1B4_67 are separated by 152 nucleotidesPf1N1B4_67 and Pf1N1B4_68 overlap by 1 nucleotides Pf1N1B4_65 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 67,925 to 68,755 _65 Pf1N1B4_66 - Copper tolerance protein, at 68,817 to 69,353 _66 Pf1N1B4_67 - Copper-sensing two-component system response regulator CusR, at 69,506 to 70,186 _67 Pf1N1B4_68 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 70,186 to 71,535 _68 Position (kb) 69 70 71Strain fitness (log2 ratio) -2 -1 0 1 2 3at 68.619 kb on + strand, within Pf1N1B4_65at 68.619 kb on + strand, within Pf1N1B4_65at 68.619 kb on + strandat 68.620 kb on - strand, within Pf1N1B4_65at 68.620 kb on - strand, within Pf1N1B4_65at 68.698 kb on - strandat 68.698 kb on - strandat 68.709 kb on + strandat 68.710 kb on - strandat 68.871 kb on - strand, within Pf1N1B4_66at 69.038 kb on + strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strand, within Pf1N1B4_66at 69.045 kb on - strandat 69.045 kb on - strand, within Pf1N1B4_66at 69.129 kb on + strand, within Pf1N1B4_66at 69.130 kb on - strand, within Pf1N1B4_66at 69.130 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strand, within Pf1N1B4_66at 69.141 kb on - strandat 69.150 kb on + strand, within Pf1N1B4_66at 69.207 kb on - strand, within Pf1N1B4_66at 69.207 kb on - strand, within Pf1N1B4_66at 69.271 kb on + strand, within Pf1N1B4_66at 69.330 kb on + strandat 69.330 kb on + strandat 69.395 kb on + strandat 69.432 kb on + strandat 69.432 kb on + strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.433 kb on - strandat 69.473 kb on + strandat 69.474 kb on - strandat 69.474 kb on - strandat 69.476 kb on - strandat 69.476 kb on - strandat 69.639 kb on - strand, within Pf1N1B4_67at 69.729 kb on + strand, within Pf1N1B4_67at 69.798 kb on - strand, within Pf1N1B4_67at 70.129 kb on + strandat 70.160 kb on + strandat 70.161 kb on - strandat 70.534 kb on + strand, within Pf1N1B4_68at 70.596 kb on - strand, within Pf1N1B4_68at 71.044 kb on + strand, within Pf1N1B4_68at 71.044 kb on + strand, within Pf1N1B4_68at 71.045 kb on - strand, within Pf1N1B4_68at 71.045 kb on - strand, within Pf1N1B4_68

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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68,619 + Pf1N1B4_65 0.84 -1.1
68,619 + Pf1N1B4_65 0.84 -0.5
68,619 + +0.3
68,620 - Pf1N1B4_65 0.84 -0.2
68,620 - Pf1N1B4_65 0.84 +1.5
68,698 - +0.9
68,698 - +0.7
68,709 + +0.1
68,710 - +1.9
68,871 - Pf1N1B4_66 0.10 -0.1
69,038 + Pf1N1B4_66 0.41 -0.1
69,045 - Pf1N1B4_66 0.42 -0.9
69,045 - Pf1N1B4_66 0.42 -1.9
69,045 - Pf1N1B4_66 0.42 -0.1
69,045 - Pf1N1B4_66 0.42 +0.9
69,045 - -0.1
69,045 - Pf1N1B4_66 0.42 -0.3
69,129 + Pf1N1B4_66 0.58 -0.1
69,130 - Pf1N1B4_66 0.58 -1.6
69,130 - Pf1N1B4_66 0.58 -0.1
69,141 - Pf1N1B4_66 0.60 -1.2
69,141 - Pf1N1B4_66 0.60 -0.2
69,141 - Pf1N1B4_66 0.60 +0.5
69,141 - Pf1N1B4_66 0.60 -0.1
69,141 - +0.5
69,150 + Pf1N1B4_66 0.62 -0.5
69,207 - Pf1N1B4_66 0.73 +0.7
69,207 - Pf1N1B4_66 0.73 +1.2
69,271 + Pf1N1B4_66 0.85 -1.7
69,330 + +0.3
69,330 + -0.1
69,395 + -0.4
69,432 + +0.3
69,432 + +1.1
69,433 - +0.4
69,433 - -0.1
69,433 - -0.3
69,433 - +2.9
69,473 + -0.1
69,474 - -1.4
69,474 - +0.2
69,476 - -1.2
69,476 - +1.2
69,639 - Pf1N1B4_67 0.20 -1.0
69,729 + Pf1N1B4_67 0.33 -0.1
69,798 - Pf1N1B4_67 0.43 +0.2
70,129 + -0.5
70,160 + +0.9
70,161 - -2.2
70,534 + Pf1N1B4_68 0.26 +0.5
70,596 - Pf1N1B4_68 0.30 +1.8
71,044 + Pf1N1B4_68 0.64 -0.1
71,044 + Pf1N1B4_68 0.64 +1.3
71,045 - Pf1N1B4_68 0.64 +0.4
71,045 - Pf1N1B4_68 0.64 -0.1

Or see this region's nucleotide sequence