Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5971 and Pf1N1B4_5972 overlap by 8 nucleotides Pf1N1B4_5972 and Pf1N1B4_5973 are separated by 10 nucleotides Pf1N1B4_5973 and Pf1N1B4_5974 overlap by 8 nucleotides
Pf1N1B4_5971 - 2-Methylcitrate dehydratase AcnD, at 418,726 to 420,504
_5971
Pf1N1B4_5972 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 420,497 to 421,162
_5972
Pf1N1B4_5973 - Probable permease of ABC transporter, at 421,173 to 421,823
_5973
Pf1N1B4_5974 - ABC-type polar amino acid transport system, ATPase component, at 421,816 to 422,538
_5974
Position (kb) 420
421
422 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 419.527 kb on + strand at 419.527 kb on + strand, within Pf1N1B4_5971 at 419.527 kb on + strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand, within Pf1N1B4_5971 at 419.528 kb on - strand at 419.528 kb on - strand at 419.528 kb on - strand at 419.529 kb on + strand, within Pf1N1B4_5971 at 419.680 kb on + strand, within Pf1N1B4_5971 at 419.680 kb on + strand, within Pf1N1B4_5971 at 419.819 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.042 kb on - strand, within Pf1N1B4_5971 at 420.192 kb on + strand at 420.352 kb on + strand at 420.352 kb on + strand at 420.352 kb on + strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.353 kb on - strand at 420.507 kb on - strand at 420.507 kb on - strand at 420.720 kb on - strand, within Pf1N1B4_5972 at 420.720 kb on - strand, within Pf1N1B4_5972 at 420.729 kb on - strand, within Pf1N1B4_5972 at 421.040 kb on + strand, within Pf1N1B4_5972 at 421.041 kb on - strand, within Pf1N1B4_5972 at 421.094 kb on + strand, within Pf1N1B4_5972 at 421.095 kb on - strand, within Pf1N1B4_5972 at 421.095 kb on - strand, within Pf1N1B4_5972 at 421.098 kb on - strand at 421.458 kb on + strand, within Pf1N1B4_5973 at 421.470 kb on + strand, within Pf1N1B4_5973 at 421.748 kb on + strand, within Pf1N1B4_5973 at 421.748 kb on + strand, within Pf1N1B4_5973 at 421.749 kb on - strand, within Pf1N1B4_5973 at 421.753 kb on - strand, within Pf1N1B4_5973 at 421.755 kb on + strand, within Pf1N1B4_5973 at 421.755 kb on + strand, within Pf1N1B4_5973 at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.835 kb on + strand at 421.836 kb on - strand at 421.836 kb on - strand at 421.836 kb on - strand at 421.839 kb on + strand at 421.839 kb on + strand at 421.839 kb on + strand at 421.840 kb on - strand at 421.840 kb on - strand at 421.840 kb on - strand at 421.840 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 419,527 + +0.8 419,527 + Pf1N1B4_5971 0.45 +0.8 419,527 + Pf1N1B4_5971 0.45 +0.5 419,528 - Pf1N1B4_5971 0.45 -0.1 419,528 - Pf1N1B4_5971 0.45 -1.2 419,528 - Pf1N1B4_5971 0.45 -0.4 419,528 - Pf1N1B4_5971 0.45 -1.2 419,528 - Pf1N1B4_5971 0.45 +0.8 419,528 - +0.0 419,528 - +0.8 419,528 - -0.6 419,529 + Pf1N1B4_5971 0.45 +3.1 419,680 + Pf1N1B4_5971 0.54 -0.7 419,680 + Pf1N1B4_5971 0.54 +0.4 419,819 - Pf1N1B4_5971 0.61 -1.0 420,042 - Pf1N1B4_5971 0.74 -0.8 420,042 - Pf1N1B4_5971 0.74 -0.7 420,042 - Pf1N1B4_5971 0.74 -1.7 420,042 - Pf1N1B4_5971 0.74 -0.2 420,192 + -1.6 420,352 + -1.2 420,352 + -2.0 420,352 + -1.1 420,353 - +0.6 420,353 - +0.4 420,353 - -1.8 420,353 - -0.2 420,507 - +1.4 420,507 - +0.4 420,720 - Pf1N1B4_5972 0.33 -1.8 420,720 - Pf1N1B4_5972 0.33 -1.8 420,729 - Pf1N1B4_5972 0.35 -0.6 421,040 + Pf1N1B4_5972 0.82 +0.0 421,041 - Pf1N1B4_5972 0.82 +2.4 421,094 + Pf1N1B4_5972 0.90 -1.2 421,095 - Pf1N1B4_5972 0.90 -0.6 421,095 - Pf1N1B4_5972 0.90 -1.4 421,098 - -0.5 421,458 + Pf1N1B4_5973 0.44 -1.8 421,470 + Pf1N1B4_5973 0.46 +0.4 421,748 + Pf1N1B4_5973 0.88 -0.2 421,748 + Pf1N1B4_5973 0.88 +0.4 421,749 - Pf1N1B4_5973 0.88 +0.8 421,753 - Pf1N1B4_5973 0.89 +0.5 421,755 + Pf1N1B4_5973 0.89 -1.7 421,755 + Pf1N1B4_5973 0.89 +0.6 421,835 + +0.6 421,835 + +1.1 421,835 + +1.0 421,835 + -0.2 421,835 + -0.8 421,835 + +0.0 421,836 - +1.5 421,836 - +0.5 421,836 - -0.4 421,839 + -2.0 421,839 + -0.8 421,839 + -0.2 421,840 - +0.8 421,840 - -0.2 421,840 - +0.0 421,840 - -0.1
Or see this region's nucleotide sequence